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pMC-ME_CreER-pA-FRT-acry:mCherry-pA-FRT

Plasmid

#200544

Purpose

mini circle middle entry plasmid for CreER-pA-FRT-acry: mCherry -pA-FRT with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

CreER, mCherry

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_P2A-CreER-pA ORF1 FRT-acry:mCherry-pA-FRT

Plasmid

#200545

Purpose

mini circle middle entry plasmid for P2A-CreER-pA (ORF 1) FRT-acry:mCherry-pA-FRT with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

CreER, mCherry

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_P2A-CreER-pA ORF2 FRT-acry:mCherry-pA-FRT

Plasmid

#200546

Purpose

mini circle middle entry plasmid for P2A-CreER-pA (ORF 2) FRT-acry:mCherry-pA-FRT with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

CreER, mCherry

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_P2A-CreER-pA ORF3 FRT-acry:mCherry-pA-FRT

Plasmid

#200547

Purpose

mini circle middle entry plasmid for P2A-CreER-pA (ORF 3) FRT-acry:mCherry-pA-FRT with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

CreER, mCherry

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME T2A-mCherry-P2A-NTRv2-pA ORF-1

Plasmid

#200529

Purpose

mini circle middle entry plasmid for T2A- mCherry- P2A- ntr version 2- pA (ORF 1) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry, NTRv2

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME T2A-mCherry-P2A-NTRv2-pA ORF-2

Plasmid

#200530

Purpose

mini circle middle entry plasmid for T2A- mCherry- P2A- ntr version 2- pA (ORF 2) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry, NTRv2

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMC-ME_T2A-mCherry-P2A-NTRv2-pA ORF-3

Plasmid

#200531

Purpose

mini circle middle entry plasmid for T2A- mCherry- P2A- ntr version 2- pA (ORF 3) with flanking BsaI cut sites

Depositor

Junsu Kang

Article

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Insert

mCherry, NTRv2

Use

Mini-golden

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pTol1-EcoRI-BmtI-BamHI-cmlc2-nmKate

Plasmid

#238372

Purpose

Empty backbone to generate guideRNA shuttles, that also drives expression of nuclear mKate in cardiomyocytes.

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nmKate

Promoter

cmlc2 (myl7)

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pTol1-Spe-KpnI-ubb-nmKate

Plasmid

#238373

Purpose

Empty backbone to generate guideRNA shuttles, that also drives ubiquitous expression of nuclear mKate.

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nmKate

Promoter

ubiquitin

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_HS∆P2

Plasmid

#225197

Purpose

Used to create a markerless deletion of the E. coli HS P2 phage

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

P2 phage homology arms

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTn7xTS_SpyCatcher:mNG

Plasmid

#225185

Purpose

Used for inserting SpyCatcher fused to mNeonGreen at the bacterial Tn7 site

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

SpyCatcher:mNeonGreen

Tags

SpyCatcher

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTn7xTS_SpyCatcher:AausFP1

Plasmid

#225186

Purpose

Used for inserting SpyCatcher fused to AausFP1 at the bacterial Tn7 site

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

SpyCatcher:AausFP1

Tags

SpyCatcher

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTn7xTS_SpyCatcher:mCitrine

Plasmid

#225187

Purpose

Used for inserting SpyCatcher fused to mCitrine at the bacterial Tn7 site

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

SpyCatcher:mCitrine

Tags

SpyCatcher

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTn7xTS_SpyCatcher:mTFP1

Plasmid

#225189

Purpose

Used for inserting SpyCatcher fused to mTFP1 at the bacterial Tn7 site

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

SpyCatcher:mTFP1

Tags

SpyCatcher

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTn7xTS_PrecA:mNeonGreen

Plasmid

#225192

Purpose

Used for inserting PrecA upstream of mNeonGreen at the bacterial Tn7 site for use as a RecA reporter

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

PrecA:mNeonGreen

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+-drAgo2

Plasmid

#221711

Purpose

Expression of zebrafish Ago2 in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2 (ago2 Zebrafish)

Use

Fish expression

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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Tol2_-87prox1a:basEGFP_Acry:GFP

Plasmid

#218213

Purpose

Zebrafish -87prox1a enhancer reporter in the lymphatics at 5 dpf. Acry_GFP as a control in the lens

Depositor

Katarzyna Koltowska

Article

Panara et al Development. 2024 May 1;151(9):dev202525

Insert

-87prox1a

Use

Zebrafish expression

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pME rras_Q61R

Plasmid

#201057

Purpose

Gateway middle entry vector expressing constitutively actve zebrafish rras

Depositor

Sarah Childs

Article

Greysson-Wong et al Development. 2023 Sep 15;150(18):dev2018

Insert

RRas_Q61R (rras Zebrafish)

Use

Zebrafish expression

Mutation

Mutation Q61R, constitutively active

Promoter

none

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-D-Topo-kcnk17

Plasmid

#208999

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk17 (kcnk17 Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-D-Topo-kcnk5a

Plasmid

#208988

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk5a (kcnk5a Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk6

Plasmid

#208990

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk6 (kcnk6 Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-D-Topo-kcnk9

Plasmid

#208992

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk9 (kcnk9 Zebrafish)

Use

Cloning

Mutation

Without STOP codon

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk13a

Plasmid

#208996

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Article

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk13a (kcnk13a Zebrafish)

Use

Cloning

Promoter

T7

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCK005 ubi:mCerulean_ubb-polyA

Plasmid

#195979

Purpose

Expression construct

Depositor

Alexa Burger, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

ubi:mCerulean

Use

Other

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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50_p5E_UBI_loxTagBFP

Plasmid

#172518

Purpose

p5E tol2kit compatible clone encoding a Ubiquitin promoter and a tagBFP sequence terminated by 3x polyA sequences and flanked by floxP sites for CRE-excision

Depositor

Jean Giacomotto

Article

Guo et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

ubiquitin promoter, TagBFP, loxP sites (inversion)

Promoter

Ubiquitin

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnj12a

Plasmid

#173141

Purpose

This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

Insert

kcnj12a (kcnj12a Zebrafish)

Use

Pcr cloning vector

Promoter

No promoter at 5 end; T7 promoter at 3 end.

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-D-kcnj16

Plasmid

#173146

Purpose

This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

Insert

kcnj16a (kcnj16 Zebrafish)

Use

Pcr cloning vector

Mutation

Stop codon removed

Promoter

No promoter at 5 end; T7 promoter at 3 end.

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnj19b

Plasmid

#173149

Purpose

This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

Insert

kcnj19b (kcnj19b Zebrafish)

Use

Pcr cloning vector

Promoter

No promoter at 5 end; T7 promoter at 3 end.

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-D-kcnj1a.1

Plasmid

#173125

Purpose

This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

Insert

kcnj1a (kcnj1a.1 Zebrafish)

Use

Pcr cloning vector

Promoter

No promoter at 5 end; T7 promoter at 3 end.

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnj11l

Plasmid

#173140

Purpose

This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

Insert

kcnj11l (kcnj11l Zebrafish)

Use

Pcr cloning vector

Promoter

No promoter at 5 end; T7 promoter at 3 end.

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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