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KEE +E111H

Plasmid

#187431

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Publication

Mohammadi et al PLoS Genet. 2022 Aug 16;18(8):e1010323.

Inserts

ATP1A1
ATP1B1

Expression

Insect

Mutation

changed glutamic acid at 117 to histidine

Promoter

PH and p10

Available since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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FER +H122D

Plasmid

#187428

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Publication

Mohammadi et al PLoS Genet. 2022 Aug 16;18(8):e1010323.

Inserts

ATP1A1
ATP1B1

Expression

Insect

Mutation

changed histidine at 128 to aspartic acid

Promoter

PH and p10

Available since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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CHI +N122D

Plasmid

#187427

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Publication

Mohammadi et al PLoS Genet. 2022 Aug 16;18(8):e1010323.

Inserts

ATP1A1
ATP1B1

Expression

Insect

Mutation

changed asparagine at 130 to aspartic acid

Promoter

PH and p10

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pQQ95

Plasmid

#254494

Purpose

NLP7 nitrate sensor built based on pQQ7, pQQ90, and pQQ132

Depositor

Gözde Demirer

Publication

Li et al Proc Natl Acad Sci U S A. 2026 Jul 28;12

Insert

pCsC_F_pOlesin_FASTred+F_p4×[NLP7-TATA]_pMinDFR_GeNL[ST-LS1 intron2_v3]_THsp18+R_pNOS_NLuc[ST-LS1 intron2_v3]_Tnos

Expression

Plant

Available since

Oct. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pFBDMchmTet1CD (pc2859)

Plasmid

#246925

Purpose

Expresses mCherry-tagged catalytic active domain of mouse Tet1 in mammalian cells. N-terminal tagged to mCherry.

Depositor

Cristina Cardoso

Publication

Ludwig et al Nucleic Acids Res. 2017 Mar 17;45(5):243

Insert

Tet1 (Tet1 Mouse)

Tags

mcherry

Expression

Insect

Mutation

aa 1365-2057

Promoter

Polyhedrin Promoter

Available since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP-Prs.AEBP2iso2.mt1∆2∆

Plasmid

#247014

Purpose

Expresses human AEBP2iso2.mt1∆2∆ in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Publication

Mucha et al EMBO J. 2025 Dec;44(23):6979-7020. doi:

Insert

AEBP2iso2.mt1∆2∆ (AEBP2 Human)

Tags

6xHis-MBP

Expression

Insect

Mutation

deletion of aa 37-48 and 95-109

Promoter

Polyhedrin

Available since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP-Prs.AEBP2iso2.mt1∆

Plasmid

#247008

Purpose

Expresses human AEBP2iso2.mt1∆ in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Publication

Mucha et al EMBO J. 2025 Dec;44(23):6979-7020. doi:

Insert

AEBP2iso2.mt1∆ (AEBP2 Human)

Tags

6xHis-MBP

Expression

Insect

Mutation

deletion of aa 37-48

Promoter

Polyhedrin

Available since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP-Prs.AEBP2iso2.mt2∆

Plasmid

#247011

Purpose

Expresses human AEBP2iso2.mt2∆ in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Publication

Mucha et al EMBO J. 2025 Dec;44(23):6979-7020. doi:

Insert

AEBP2iso2.mt2∆ (AEBP2 Human)

Tags

6xHis-MBP

Expression

Insect

Mutation

deletion of aa 95-109

Promoter

Polyhedrin

Available since

Nov. 20, 2025

Availability

Academic Institutions and Nonprofits only

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Halo-ATG2A HRD

Plasmid

#207540

Purpose

Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG2A locus in human cells

Depositor

Jens Schmidt

Publication

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

HaloTag flanked by human ATG2A locus sequences

Tags

HaloTag

Expression

Mammalian

Promoter

Endogenous

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Halo-ATG5 HRD

Plasmid

#207542

Purpose

Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG5 locus in human cells

Depositor

Jens Schmidt

Publication

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

HaloTag flanked by human ATG5 locus sequences

Tags

HaloTag

Expression

Mammalian

Promoter

Endogenous

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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TR knockout HRD

Plasmid

#207579

Purpose

Homologous recombination donor replacing TR with a puro cassette.

Depositor

Jens Schmidt

Publication

Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

Insert

puro resistance cassette flanked by TR locus sequences excluding TR

Tags

None

Expression

Mammalian

Promoter

SV40

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL_UTP15:1-370

Plasmid

#210937

Purpose

Insect expression of human UTP15 fragment M1 to H370. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

UTP15:M1-H370 (UTP15 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_WDR73:1-378

Plasmid

#210932

Purpose

Insect expression of full-length human WDR73. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR73:M1-R378 (WDR73 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_PPWD1:80-453

Plasmid

#210911

Purpose

Insect expression of human PPWD1 fragment A80 to K453. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

PPWD1:A80-K453 (PPWD1 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_CORO1B:1-489

Plasmid

#210906

Purpose

Insect expression of full-length human CORO1B. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CORO1B:M1-A489 (CORO1B Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_RBBP7:1-411

Plasmid

#210898

Purpose

Insect expression of human RBBP7 fragment M1 to E411. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

RBBP7:M1-E411 (RBBP7 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_WDR41_30-441

Plasmid

#210895

Purpose

Insect expression of human WDR41 fragment Q30 to L441. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR41:Q30-L441 (WDR41 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBOH-MHL_WDR41_30-459

Plasmid

#210894

Purpose

Insect expression of human WDR41 fragment Q30 to V459. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR41:Q30-V459 (WDR41 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFB-MBD2.4Ch (pc2393)

Plasmid

#211730

Purpose

Expresses MBD2 (aa236-414) mCherry tagged in insect cells

Depositor

Cristina Cardoso

Publication

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD2.4 aa236-414

Tags

mCherry

Expression

Insect

Promoter

Polyhedrin promotor

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pFB-MBD2.3Ch (pc2392)

Plasmid

#211729

Purpose

Expresses MBD2 (aa222-414) mCherry tagged in insect cells

Depositor

Cristina Cardoso

Publication

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD2.3 aa222-414

Tags

mCherry

Expression

Insect

Promoter

Polyhedrin promotor

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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