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C3 luc (T1 del)
Plasmid#11358DepositorInsertC3 promoter (C3 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AU1-mTOR-E2419K
Plasmid#19994DepositorInsertmammalian target of rapamycin-E2419K (Mtor Rat)
UseTagsAU1ExpressionMammalianMutationChanged Glutamine 2419 to LysinePromoterAvailable sinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
FRB_LD 0_CS G_tTA-BFP
Plasmid#58876PurposeMESA target chain with Rapamycin FRB ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with FRB ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pATmin-GG
Plasmid#191352PurposeClostridium expression vector (pAMB1 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
UseTagsExpressionBacterialMutationPromoterPlacAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
FRB_nSuMMVp
Plasmid#118970PurposeExpresses N-terminus of sunflower mild mosaic virus protease (SuMMVp) fused to the FKBP-rapamycin binding (FRB) domainDepositorInsertsplit nSuMMVp in fusion with FRB
UseTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
N1-mSin1.2-GFP
Plasmid#72908PurposeGFP-tagged mSin1 (isoform 2)DepositorInsertTarget of rapamycin complex 2 subunit MAPKAP1, isoform 2 (MAPKAP1 Human)
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCPP5052
Plasmid#128729PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor cloneDepositorInserthopAM1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJK511
Plasmid#155384PurposedCas12a oscillator, reverse direction crRNAs. dCas12a (D917A) + PA4-mVenusDepositorInsertsdCas12a (F. novicida)
PA4-mVenus
dCas12a oscillator crRNAs
UseCRISPRTagsExpressionBacterialMutationD917A (nuclease-deactivating)PromoterAvailable sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AU1-mTOR-L1460P
Plasmid#19995DepositorInsertmammalian target of rapamycin-L1460P (Mtor Rat)
UseTagsAU1ExpressionMammalianMutationChanged Leucine 1460 to ProlinePromoterAvailable sinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
LBR-FKBP-GFP
Plasmid#222413PurposeTo express LBR with C-terminal GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cellsDepositorInsertLBR (LBR Human)
UseTagsFKBP-GFPExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 S6K2 E388 D3E
Plasmid#17731DepositorInsertS6K2 (RPS6KB2 Human)
UseTagsHAExpressionMammalianMutationT388E, D3E(S410D, S417D, S423D): rapamycin-resist…PromoterCMVAvailable sinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
FRB_nSbMVp
Plasmid#118969PurposeExpresses N-terminus of soybean mosaic virus protease (SbMVp) fused to FKBP-rapamycin binding (FRB) domainDepositorInsertsplit nSbMVp in fusion with FRB
UseTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
N1-mSin1.5-GFP
Plasmid#72909PurposeGFP-tagged mSin1 (isoform 5)DepositorInsertTarget of rapamycin complex 2 subunit MAPKAP1, isoform 5 (MAPKAP1 Human)
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
RapR-Shp2-mVenus-flag
Plasmid#188655PurposeRapamycin regulated Shp2 phosphatase with mVenus and flag tagDepositorInsertShp2
UseTagsFlag and mVenusExpressionMammalianMutationiFKBP insertion at V406 with deletion of residues…PromoterCMVAvailable sinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
FKBP-CD28TMD-NTEVp(H75S)
Plasmid#137828PurposeMESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75S mutant TEV proteaseDepositorInsertFKBP-CD28TMD-NTEVp(H75S)
UseTags3x FLAGExpressionMammalianMutationMutated Histidine 75 to Serine in NTEVp fragmentPromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK503
Plasmid#155380PurposedCas12a oscillator, same direction crRNAs. dCas12a (D917A) + PA4-mVenusDepositorInsertsdCas12a (F. novicida)
PA4-mVenus
dCas12a oscillator crRNAs
UseCRISPRTagsExpressionBacterialMutationD917A (nuclease-deactivating)PromoterAvailable sinceMay 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA
Plasmid#137827PurposeMESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal WT TEV protease, M cleavage sequence, and tTA transcription factorDepositorInsertFRB-CD28TMD-CTEVp(WT)-CS(M)-tTA
UseTags3x FLAGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
FRB_nPPVp
Plasmid#118966PurposeExpresses N-terminus of plum pox virus protease (PPVp) fused to FKBP-rapamycin binding (FRB) domainDepositorInsertsplit nPPVp in fusion with FRB
UseTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
gCOTS-pyl
Plasmid#92048PurposeThis is a S. elongatus (PCC7942) shuttle vector used for recombination of the pylRS orthogonal translation system to genomic neutral site 2.DepositorInsertsPyrrolysyl tRNA(cua)
Pyrrolysyl tRNA synthetase (methanosarcina mazei)
UseCyanobacteria s. elongatus genome recombination i…TagsExpressionMutationPromoterLeuP (native S. elongatus promoter) and PrcbLAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only
