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pPB_mU6_enh-gRNA_Puro-T2A-BFP

Plasmid

#235571

Purpose

Enhanced guide RNA expression & genomic integration. Target gRNA sequences are cloned in via the BstXI and BlpI sites.

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

sgRNA backbone

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD-mWatermelon

Plasmid

#190625

Purpose

Bacterial expression of mWatermelon GFP

Depositor

Robert Campbell

Article

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

mWatermelon

Expression

Bacterial

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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ncvB-ssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Plasmid

#140168

Purpose

Expresses a regulatable secretory cargo for Tetrahymena thermophila

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

ssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Use

Other

Promoter

MTT1

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Tnnt2-HA-Luciferase

Plasmid

#227803

Purpose

cardiomyocytes-specific plasmid with Tnnt2 promotor, expressing the coding sequence of luciferase and a HA tag

Depositor

Yuxuan Guo

Article

Chen et al Fundam Res. 2025 Jun 30. doi: https://do

Insert

HA-Luciferase

Use

AAV

Expression

Mammalian

Promoter

Tnnt2

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pEarleyGate100-pSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Plasmid

#140170

Purpose

Expresses a regulatable secretory cargo for Arabidopsis thaliana

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Expression

Plant

Promoter

CaMV 35S

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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pEarleyGate100-pSpoA-DsRed-Express2-FKBP(LV)(C22V)

Plasmid

#140169

Purpose

Expresses a regulatable secretory cargo (without an ER export signal) for Arabidopsis thaliana

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pSpoA-DsRed-Express2-FKBP(LV)(C22V)

Expression

Plant

Promoter

CaMV 35S

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac204-pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)

Plasmid

#140155

Purpose

Expresses a regulatable secretory cargo for yeast at the TRP1 locus

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)

Expression

Yeast

Promoter

TPI1

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCXLE-mCherry

Plasmid

#193293

Purpose

episomal vector expressing mCherry fluorescent protein

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Setd2CD

Plasmid

#235579

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Setd2 fused with scFV to programme H3K36me3

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Setd2 (Setd2 Mouse)

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Ring1bCD

Plasmid

#235574

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Ring1b fused with scFV to programme H2AK119ub.

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Ring1b (Rnf2 Mouse)

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCCAT1-Red

Plasmid

#226521

Purpose

CCAT1 transgene with fluorescent protein mKate2

Depositor

Neville Sanjana

Article

Caragine et al Nat Commun. 2025 Feb 13;16(1):1611. doi:

Insert

EF1a_mKate2_CMV_CCAT1 (CCAT1 Human)

Use

Lentiviral

Tags

mKate2

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Ring1bCD-CatMut

Plasmid

#235583

Purpose

Dox-inducible expression of control catalytic-mutant Ring1b CD fused with scFV

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Ring1b (Rnf2 Mouse)

Use

CRISPR

Mutation

I53S

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD-SPW

Plasmid

#190628

Purpose

Bacterial expression of genetically encoded protease indicator SPW

Depositor

Robert Campbell

Article

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

SPW

Expression

Bacterial

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA-SCW

Plasmid

#190633

Purpose

Mammalian expression of genetically encoded calcium indicator SCW

Depositor

Robert Campbell

Article

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

SCW

Expression

Mammalian

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-SKW

Plasmid

#190631

Purpose

Bacterial expression of genetically encoded potassium indicator SKW

Depositor

Robert Campbell

Article

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

SKW

Expression

Bacterial

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD-SCW

Plasmid

#190626

Purpose

Bacterial expression of genetically encoded calcium indicator SCW

Depositor

Robert Campbell

Article

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

SCW

Expression

Bacterial

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTol1-EcoRI-BmtI-BamHI-cmlc2-nmKate

Plasmid

#238372

Purpose

Empty backbone to generate guideRNA shuttles, that also drives expression of nuclear mKate in cardiomyocytes.

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nmKate

Promoter

cmlc2 (myl7)

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Setd2CD-CatMut

Plasmid

#235588

Purpose

Dox-inducible expression of control catalytic-mutant Setd2 CD fused with scFV

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Setd2 (Setd2 Mouse)

Use

CRISPR

Mutation

R1599C

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pMaSat-miRFP670 pc3944

Plasmid

#201695

Purpose

Expresses of miRFP-tagged MaSat in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

MaSat Synthetic construct zinc finger

Tags

miRFP670

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA-SCcpV

Plasmid

#190636

Purpose

Mammalian expression of genetically encoded calcium indicator SCcpV

Depositor

Robert Campbell

Article

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

SCcpV

Expression

Mammalian

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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