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We narrowed to 4,639 results for: crispr c plasmids

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Showing: 1741 - 1760 of 4639 results

  3. pAsCpf1GG

    Plasmid
    #131011
    Purpose
    Backbone plasmid for generating CRISPR arrays for AsCas12a. Contains a GFP-dropout cassette and a direct repeat.
    Depositor
    Chase Beisel
    Article
    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:
    Inserts
    direct repeat of AsCas12a
    GFP expression cassette
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    131011-plasmid-map-sequence-id-258106

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  4. pCMV-T7-SpCas9-NG-P2A-mScarlet (MNW005)

    Plasmid
    #174140
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…
    Promoter
    CMV and T7
    Available Since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174140-plasmid-map-sequence-id-341918

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  5. pSL1765 (pSPIN-R, R*MmeI)

    Plasmid
    #160734
    Purpose
    Single-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    160734-plasmid-map-sequence-id-318595

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  7. lentiSAM v2 (Puro)

    Plasmid
    #92062
    Purpose
    Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Adam Karpf
    Article
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 (sgRNA) and EF1a (dCas9-VP64)
    Available Since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92062-plasmid-map-sequence-id-180159

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  8. pPPC011

    Plasmid
    #171143
    Purpose
    Expression of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) on pRK2-KmR plasmid
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(
    Insert
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    SoxS has R93A and S101A mutations
    Promoter
    Sp.pCas9, BBa_J23107
    Available Since
    Oct. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    171143-plasmid-map-sequence-id-345216

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  10. (915) SB EF1a dCasphi-[myc-ZIM3-HA] U6-gStop

    Plasmid
    #195623
    Purpose
    Sleeping Beauty EF1a plasmid for constitutive expression of dCasphi-ZIM3 CRISPRi construct
    Depositor
    Tonia Rex
    Insert
    dCasphi
    Tags
    C-Myc (NLS), HA tag, and ZIM3
    Expression
    Mammalian
    Mutation
    P749A and P753A
    Promoter
    EF1a (Mlu1/EcoR1)
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    195623-plasmid-map-sequence-id-385117

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  11. pDD286 (TagRFP^SEC^3xMyc)

    Plasmid
    #70684
    Purpose
    TagRFP-T^SEC^3xMyc vector with ccdB sites for cloning homology arms.
    Depositor
    Bob Goldstein
    Insert
    TagRFP-T-C1^SEC^3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xMyc and C. elegans codon-optimized TagRFP-T
    Expression
    Worm
    Available Since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    70684-plasmid-map-sequence-id-306412

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Showing: 1741 - 1760 of 4639 results