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pLentiCRISPRv2_IFNB1_gRNA_3

Plasmid

#235533

Purpose

gRNA against human IFNB1

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

IFNB1 (IFNB1 Human)

Use

Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-tagBFP-2A-coICP0

Plasmid

#235536

Purpose

To express tagBFP-2A-coICP0 (co = codon-optimized)

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

ICP0

Use

Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-tagRFP657-2A-coICP0

Plasmid

#235538

Purpose

To express tagRFP657-2A-coICP0 (co = codon-optimized)

Depositor

Nicolas Manel

Article

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

ICP0

Use

Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC TtoS

Plasmid

#226608

Purpose

All threonines to serine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 4QQ->GG (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

4 QQ pairs replaced with GG pairs

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC QtoA

Plasmid

#226609

Purpose

All glutamines to alanine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

6*His-TEV-FUS LC QtoA (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

All glutamine to alanine

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC-RGG1 StoG

Plasmid

#226610

Purpose

All serines in the LC domain to glycine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC-RGG1 StoG (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

All serines to glycine in the LC domain

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC-RGG1 TtoS

Plasmid

#226611

Purpose

All threonines in the LC domain to serine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC-RGG1 TtoS (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

All threonine to serine in the LC domain

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC-RGG1 QtoA

Plasmid

#226612

Purpose

All glutamines in the LC domain to alanine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC-RGG1 QtoA (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

All glutamine to alanine in the LC domain

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC-RGG1 TtoV

Plasmid

#226613

Purpose

All threonines in the LC domain to valine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC-RGG1 TtoV (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

All threonine to valine in the LC domain

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC-RGG1 QtoG

Plasmid

#226614

Purpose

All glutamines in the LC domain to glycine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC-RGG1 QtoG (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

All glutamine to glycine in the LC domain

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC-RGG1 QtoN

Plasmid

#226615

Purpose

All glutamines in the LC domain to asparagine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC-RGG1 QtoN (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

All glutamine to asparagine in the LC domain

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC-RGG1 QtoS

Plasmid

#226616

Purpose

All glutamines in the LC domain to serine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC-RGG1 QtoS (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

All glutamine to serine in the LC domain

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC noYQ_noYT

Plasmid

#226617

Purpose

T7S / Q160S double mutation

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC noYQ_noYT (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

T7S / Q160S double mutation in LC domain to remov…

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC-RGG1 wild type

Plasmid

#226596

Purpose

Wild type sequence of FUS LC-RGG1 domains

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC-RGG1 WT (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

wild type LC-RGG1

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 12S->N

Plasmid

#226599

Purpose

12 serines to asparagines

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 12S->N (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

12 serines to asparagine

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 4S->A#1

Plasmid

#226603

Purpose

4 serines to alanine at residue positions 30, 44, 48, 53

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC S->A#1 (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

4 serines to alanine in sequence position subset …

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 4S->A#2

Plasmid

#226604

Purpose

4 serines to alanine at residue positions 70, 84, 89, 95

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 4S->A#2 (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

4 serines to alanine in sequence position subset …

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 4S->A#3

Plasmid

#226605

Purpose

4 serines to alanine at residue positions 108, 117, 119, 127

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 4S->A#3 (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

4 serines to alanine in sequence position subset …

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 8Y->S

Plasmid

#226606

Purpose

8 tyrosines to serine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 8Y->S (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

8 tyrosines to serine

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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TDP-43 _CTD_6F→A

Plasmid

#194269

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with 6F→A

Depositor

Nicolas Fawzi

Article

Mohanty et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

Insert

TDP-43 (TARDBP Synthetic, Human)

Tags

6XHistag

Expression

Bacterial

Mutation

F276A, F283A, F289A, F367A, F397A, F401A

Promoter

T7

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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