We narrowed to 2,540 results for: organization Addgene

1. ZJOM96

   Plasmid

   #133663

   Purpose

   Integration of SEC26-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast early golgi.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-SEC26-mTagBFP2 (SEC26 Budding Yeast)
   

   Tags

   mTagBFP2

   Expression

   Yeast

   Available Since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCV-IRES-GFP-Caspase-1 (C285A) -CpLxIS

   Plasmid

   #131355

   Purpose

   Expresses Caspase-1 C285A with 2 copies of pLxIS motif from STING fused to its C-ternimus

   Depositor

   Jonathan Kagan

   Article

   Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

   Insert

   Caspase-1 (C285A)-CpLxIS (Casp1 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Caspase-1 (C285A)

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBa.FRB-3myc-KAP3B

   Plasmid

   #193939

   Purpose

   Mammalian expression of KAP 3B

   Depositor

   Marvin Bentley

   Article

   Garbouchian et al Mol Biol Cell. 2022 Oct 6:mbcE22080336.

   Insert

   KAP 3B (KIFAP3 Human)
   

   Tags

   FRB

   Expression

   Mammalian

   Promoter

   Chicken Beta actin

   Available Since

   Dec. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PhyB-mCherry-NLS

   Plasmid

   #51571

   Purpose

   Constitutively express nuclear localized PhyB in Saccharomyces cerevisiae

   Depositor

   Chao Tang

   Article

   Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

   Insert

   PhyB (PHYB Mustard Weed)
   

   Tags

   NLS and mCherry

   Expression

   Yeast

   Mutation

   1-908aa only

   Promoter

   ADH1pr

   Available Since

   Aug. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. PhyB-mCherry-Htb2

   Plasmid

   #51569

   Purpose

   Constitutively express nuclear localized PhyB in Saccharomyces cerevisiae

   Depositor

   Chao Tang

   Article

   Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

   Insert

   PhyB (PHYB Mustard Weed)
   

   Tags

   HTB2 and mCherry

   Expression

   Yeast

   Mutation

   1-908aa only

   Promoter

   ADH1pr

   Available Since

   Aug. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-EF1a-V5-Sec61B

   Plasmid

   #120244

   Purpose

   Expresses V5-tagged Sec61B in lentiviral vector

   Depositor

   Larry Gerace

   Article

   Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

   Insert

   Sec61B (Sec61b Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Promoter

   EF1a

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. ZJOM141

   Plasmid

   #133669

   Purpose

   Integration of COX9-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast mitochondria.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-COX9-mTagBFP2 (COX9 Budding Yeast)
   

   Tags

   mTagBFP2

   Expression

   Yeast

   Available Since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. ZJOM2

   Plasmid

   #133633

   Purpose

   Integration of VRG4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast early golgi.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-VRG4-GFP (VRG4 Budding Yeast)
   

   Tags

   EGFP

   Expression

   Yeast

   Available Since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR_L1-BirA(R118G)-YFP-NLS-STOP-L2

   Plasmid

   #127360

   Purpose

   gateway entry vector for expressing nuclear BirA* (R118G)-YFP under a promoter of choice

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   BirA* (R118G mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NLS, V5, and YFP

   Mutation

   R118G

   Promoter

   no promoter

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETM41-EcLonA

    Plasmid

    #38330

    Depositor

    Florian Freimoser

    Article

    Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

    Insert

    Lon protease fragment (lon Escherichia coli)
    

    Tags

    His, MBP, and TEV

    Expression

    Bacterial

    Mutation

    Polyphosphate binding fragment of Lon protease. …

    Promoter

    T7

    Available Since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pCMV Aquamarine-superYFP tandem

    Plasmid

    #157776

    Purpose

    Produces a fusion between Aquamarine and superYFP. It can be used as a positive control for FRET (using FLIM)

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Type

    Empty backbone

    Tags

    Aquamarine and superYFP

    Expression

    Mammalian

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR_L1-BirA(R118G)-NES-YFP-STOP-L2

    Plasmid

    #127357

    Purpose

    gateway entry vector for expressing cytosolic BirA* (R118G)-YFP under a promoter of choice

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    BirA* (R118G mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NES, V5, and YFP

    Mutation

    R118G

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLaNGo11-1

    Plasmid

    #126053

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMH-HT_ygiF-CHAD

    Plasmid

    #127793

    Purpose

    Expresses the CHAD domain of ygiF in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    ygiF-CHAD (ygiF Escherichia coli)
    

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    N-terminal TTM domain of ygiF (residues 201-433)

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. PhyB-mCherry-SIK1

    Plasmid

    #51577

    Purpose

    Constitutively express nucleolus localized PhyB in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Tags

    SIK1 and mCherry

    Expression

    Yeast

    Mutation

    1-908aa only

    Promoter

    ADH1pr

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. PhyB-mCherry-SNF7

    Plasmid

    #51574

    Purpose

    Constitutively express endosome localized PhyB in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Tags

    SNF7 and mCherry

    Expression

    Yeast

    Mutation

    1-908aa only

    Promoter

    ADH1pr

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ZJOM108

    Plasmid

    #133665

    Purpose

    Integration of mTagBFP2-Tlg1 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-mTagBFP2-TLG1 (TLG1 Budding Yeast)
    

    Tags

    mTagBFP2

    Expression

    Yeast

    Available Since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pERB257

    Plasmid

    #67761

    Purpose

    Expresses KLC1-mCherry-eDHFR

    Depositor

    Michael Lampson

    Article

    Ballister et al Curr Biol. 2015 May 18;25(10):R407-8. do

    Insert

    KLC1-mCherry-eDHFR (Klc1 Rat)
    

    Tags

    eDHFR and mCherry

    Expression

    Mammalian

    Mutation

    contains Klc1 amino acids 1-175

    Promoter

    CAG

    Available Since

    Jan. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-EF1a-V5-Vrk2

    Plasmid

    #120240

    Purpose

    Expresses V5-tagged Vrk2 in lentiviral vector

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Insert

    Vrk2 (Vrk2 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Promoter

    EF1a

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET_RGG-RGG_withTEVsite

    Plasmid

    #124940

    Purpose

    Expression of RGG-RGG

    Depositor

    Daniel Hammer, Matthew Good, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    with TEV site between RGG domains

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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