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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 42,555 results for: cha;

Showing: 3521 - 3560 of 42555 results

  5. pLX304-KDM6A-M1190T/E1335D

    Plasmid
    #203581
    Purpose
    Expresses mutant version of KDM6A with increased oxygen affinity in mammalian cells.
    Depositor
    William Kaelin, Abhishek Chakraborty
    Article
    Chakraborty et al Science. 2019 Mar 15;363(6432):1217-1222
    Insert
    KDM6A (KDM6A Human)
    Use
    Lentiviral
    Tags
    HA-tagged
    Expression
    Mammalian
    Mutation
    Changed Methionine 1190 Threonine and Glutamic Ac…
    Promoter
    CMV
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    203581-plasmid-map-sequence-id-466509

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  7. pαH-S-GSAS-2P-N317P

    Plasmid
    #196378
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein with mutation N317P with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987
    Depositor
    Priyamvada Acharya
    Article
    Manrique et al iScience. 2023 Jan 20;26(1):105855. doi:
    Insert
    S-GSAS-2P-N317P (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV 3C cleavage site (before tags) C terminal on …
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Available since
    March 24, 2023
    Availability
    Academic Institutions and Nonprofits only

  9. pPS04

    Plasmid
    #169472
    Purpose
    Broad-host-range allelic exchange vector
    Depositor
    Christopher Marx
    Type
    Empty backbone
    Use
    Allelic exchange vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 10, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    169472-plasmid-map-sequence-id-334678

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  10. pET28 E2 LBD-Tev-E1a-His

    Plasmid
    #232117
    Purpose
    For bacterial expression of a fusion protein containing from N to C-terminus: the lipoyl-binding domain of Human E2, a TEV protease site, a peptide from Human E1a, and a His tag.
    Depositor
    Ruth Sommese
    Article
    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi
    Insert
    DBT (DBT Human)
    Use
    Tags
    6xHis, RIGHHSTSDDSSAY (AA331 to 345 (preprocessin…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232117-plasmid-map-sequence-id-465790

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  11. pVQ CMV NanoV1Mutant-2a-EGFP ferritin

    Plasmid
    #79650
    Purpose
    Expresses camelid anti-GFP nanobody fused to TRPV1 with mutant pore region and GFP-ferritin chimera fusion protein
    Depositor
    Jeffrey Friedman
    Article
    Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do
    Inserts
    transient receptor potential vanilloid 1 (Trpv1 Rat)
    camelid anti-GFP nanobody
    Enhanced green fluorescent protein
    mouse ferritin light chain (Ftl1 Mouse)
    mouse ferritin heavy chain (Fth1 Mouse)
    Use
    Adenoviral
    Tags
    Expression
    Mammalian
    Mutation
    isoleucine 679 changed to lysine
    Promoter
    CMV
    Available since
    Sept. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    79650-plasmid-map-sequence-id-156910

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  13. pVRC8400-SARS-CoV-2-RBD-L455RG496R-AVI

    Plasmid
    #160479
    Purpose
    Expresses SARS-CoV-2 RBD-L455RG496R domain with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:
    Insert
    SARS-CoV-2-RBD-L455RG496R
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    changed Leucine 455 to Arginine and changed Glyci…
    Promoter
    Available since
    Oct. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    160479-plasmid-map-sequence-id-314424

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  14. pVRC8400-SARS-CoV-2-RBD-L455RA475R-AVI

    Plasmid
    #160478
    Purpose
    Expresses SARS-CoV-2 RBD-L455RA475R domain with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:
    Insert
    SARS-CoV-2-RBD-L455RA475R
    Use
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    changed Leucine 455 to Arginine and changed Alani…
    Promoter
    Available since
    Oct. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    160478-plasmid-map-sequence-id-314422

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  15. pT18mobsacB

    Plasmid
    #72648
    Purpose
    sucrose counter-selection allelic exchange vector for Pseudomonas syringae
    Depositor
    Brian Kvitko
    Type
    Empty backbone
    Use
    Sucrose counter-selection allelic exchange vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 18, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72648-plasmid-map-sequence-id-399780

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  24. pDONR2T18ms

    Plasmid
    #72645
    Purpose
    Gateway-compatible sucrose counter-selection allelic exchange vector for Pseudomonas syringae
    Depositor
    Brian Kvitko
    Type
    Empty backbone
    Use
    Sucrose counter-selection allelic exchange vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 29, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72645-plasmid-map-sequence-id-133017

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  25. pDEST2T18ms

    Plasmid
    #72647
    Purpose
    Gateway-compatible sucrose counter-selection allelic exchange vector for Pseudomonas syringae
    Depositor
    Brian Kvitko
    Type
    Empty backbone
    Use
    Sucrose counter-selection allelic exchange vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 10, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72647-plasmid-map-sequence-id-139809

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  27. AiP15385: pAAV-AiE0873m_3xC2-minBG-mScarlet-WPRE3-BGHpA (Alias: CN5385)

    Plasmid
    #230875
    Purpose
    AiE0873m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal cholinergic interneurons
    Depositor
    The Allen Institute for Brain Science, Jonathan Ting
    Article
    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1
    Insert
    mScarlet
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    230875-plasmid-map-sequence-id-459525

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Showing: 3521 - 3560 of 42555 results