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MXS_Tet

Plasmid

#62422

Purpose

MXS_chaining vector with Tet

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Tetracycline inducible promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup133-1

Plasmid

#87334

Purpose

To express shRNA against human Nup133

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup133

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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A104T DJ-1 pET15b

Plasmid

#60680

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.

Insert

A104T DJ-1 pET15b (PARK7 Human)

Tags

6xHis

Expression

Bacterial

Mutation

A104T

Promoter

T7

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
MXS_tTA2

Plasmid

#62429

Purpose

MXS_chaining vector with rTA2

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Tetracycline transactivator

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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His6_BcLOV4_C292A_mCherry_BamUK

Plasmid

#119761

Purpose

Encodes mCherry-tagged BcLOV4 "dark" mutant with C292A mutation for expression in bacterial cells

Depositor

Brian Chow

Article

Glantz et al Methods Enzymol. 2019;622:249-270. doi:

Insert

BcLOV4-C292A_mCherry

Tags

mCherry

Expression

Bacterial

Mutation

Mutated cysteine at position 292 to alanine

Promoter

T7

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTSA-38

Plasmid

#17328

Depositor

Takeshi Sano

Article

Sano et al Proc Natl Acad Sci U S A. 1995 Apr 11. 9

Insert

streptavidin

Expression

Bacterial

Mutation

Truncated to include only amino acids 16-133 of m…

Available Since

Feb. 25, 2008

Availability

Academic Institutions and Nonprofits only
pME-QF2w-SV40pA

Plasmid

#155117

Purpose

Middle entry vector containing QF2w

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QF2w

Use

Gateway middle entry vector

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF3F03

Plasmid

#70962

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Utx (Utx Fly)

Use

Gateway entry vector

Available Since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only
phpRL-BCL2-FL-pA

Plasmid

#42595

Depositor

Richard Lloyd

Article

Sherrill et al J Biol Chem. 2004 Jul 9;279(28):29066-74

Insert

hairpin - renilla luciferase - BCL2 5'UTR - firefly luciferase

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pRC05

Plasmid

#114684

Purpose

FKBP-TEV-C

Depositor

Matthew Good

Article

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Insert

FKBP-TEV-C

Tags

GST

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pBM13h-sd4U

Plasmid

#179690

Purpose

Bacterial expression of base editor

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

[sd4U] UGI-TALE3-DddA-G1397-N wildtype

Expression

Bacterial

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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MXS_bGHpA

Plasmid

#62425

Purpose

MXS_chaining vector with bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Bovine growth hormone poly A

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pRC04

Plasmid

#114683

Purpose

FRB-TEV-N

Depositor

Matthew Good

Article

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Insert

FRB-TEV-N

Tags

MBP

Expression

Bacterial

Promoter

Tac Promoter

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pJZ002

Plasmid

#120231

Purpose

CRISPR-cas9 targeting in E. coli with ampicillin resistance

Depositor

Bradley Moore

Article

Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

Type

Empty backbone

Use

CRISPR

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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MXS_CAG

Plasmid

#62420

Purpose

MXS_chaining vector with CAG

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

CAG Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_minPGK

Plasmid

#62415

Purpose

MXS_chaining vector with minPGK

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Minimal PGK Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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H2B-MiniR-mEm

Plasmid

#181990

Purpose

MiniR genetically encoded tag on H2B-mEmerald to be used with MiniE labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

Insert

H2B-MiniR-mEm

Tags

mEmerald

Expression

Mammalian

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF2G08

Plasmid

#70915

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Parg (Parg Fly)

Use

Gateway entry vector

Available Since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only
pSUPER-retro-puro-shNup133-2

Plasmid

#87333

Purpose

To express shRNA against human Nup133

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup133

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF2G09

Plasmid

#70923

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

E(z) (E(z) Fly)

Use

Gateway entry vector

Available Since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only