We narrowed to 2,307 results for: neurod

1. PB-TO-oNGN2

   Plasmid

   #198397

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase, NGN2 optimization aided by IDT tool.

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Inserts

   NEUROG2 (phospho mutant, codon optimized) (NEUROG2 Human)
   puroR-T2A-mycNLS-mTagBFP2
   rtTA3G
   

   Use

   Tags

   T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Mutation

   S24A, S193A, S207A, S209A, S219A, S232A, S239A, S…

   Promoter

   CAG, EF1a, and TRE3G

   Available since

   April 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-TO-NGN2-EMX1 BSD-mApple

   Plasmid

   #182311

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 and EMX1 expression, bsd selection, mApple

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Inserts

   hNGN2-P2A-EMX1 (NEUROG2, EMX1 Human)
   bsd-T2A-mycNLS-mApple
   

   Use

   Tags

   T2A-mycNLS-mApple

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and TRE3G

   Available since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. hnRNPA2_LC_nocharge

   Plasmid

   #139118

   Purpose

   express hnRNPA2 LC with no charged residues

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   hnRNPA2 (HNRNPA2B1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   all charged residues are changed to S/Q except fo…

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. FUS_LC_withR

   Plasmid

   #139128

   Purpose

   express FUS LC with the same number and spacing of arginines as hnRNPA2 LC

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   add arginines

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. hnRNPA2_LC_nocharge_wR

   Plasmid

   #139119

   Purpose

   express hnRNPA2 LC with no charged residues with R added back

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   hnRNPA2 (HNRNPA2B1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   remove all K, most D

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. FUS_LC_charge

   Plasmid

   #139126

   Purpose

   express FUS LC with an hnRNPA2 LC-like charge patterning

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Add R, K, D to make FUS LC have similar charge pa…

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-TO-ASCL1-DLX2

   Plasmid

   #182307

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into GABAergic neuron via ASCL1 and DLX2 expression

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Inserts

   Ascl1 (Ascl1 Mouse)
   Dlx2 (Dlx2 Mouse)
   puroR-T2A-mycNLS-mTagBFP2
   

   Use

   Tags

   None and T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and pTRE3G-bidirectional

   Available since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRRLSIN.cPPT.PGK.MD(ND2bHLH)WCS.WPRE

   Plasmid

   #122056

   Purpose

   Lentiviral expression of a MyoD chimeric protein substituted with the NeuroD2 bHLH domain and point mutations to prevent MyoD interaction with PBX/MEIS: W96A, C98A, and S253P

   Depositor

   Stephen Tapscott

   Article

   Fong et al Cell Rep. 2015 Mar 31;10(12):1937-46. do

   Insert

   MD(ND2bHLH)WCS (Myod1, Neurod2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   MyoD chimeric protein substituted with the NeuroD…

   Promoter

   Available since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. FUS_LC_charge_RtoK

   Plasmid

   #139127

   Purpose

   express FUS LC with hnRNPA2-like charge patterning where all R are changed to K

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Add K and D to FUS LC so it has the same charge p…

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK5-EGFP-Tau P301L

    Plasmid

    #46908

    Purpose

    expresses EGFP tagged Tau P301L in mammalian cells

    Depositor

    Karen Ashe

    Article

    Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

    Insert

    Tau P301L (MAPT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    P301L

    Promoter

    CMV

    Available since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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