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Plasmid#192951PurposeFor membrane insertion study; Expresses sptse5-CT (1169-1300) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminusDepositorInsertsptse5-CT (1169-1300)
UseTagsPelB signal peptideExpressionBacterialMutationencodes for sptse5 (1169-1300)PromoterAvailable sinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2393-Tier1-PhCMV-p2a_fastFT
Plasmid#169530PurposeTier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "fast" maturation fastFT fused to a "self-cleaving" peptide p2a (PhCMV-p2a_medFT-pA).DepositorInsertPCMV-driven P2A-fused fluorescent timer, fast maturation time
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2334_Tier3(Lenti_inverse)-rtTA-YPet_Puro
Plasmid#169665PurposeTier-3 vector for lentiviral stable integration of the rtTA and puromyine - Ypet selection cassette in inverted direction with polyA (PRPBSA-YPet-p2a-PuroR::PPGK-rtTA-pA::A3-pA)DepositorInsertPPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
UseTagsExpressionMammalianMutationPromoterPhCMV / PPGK / RPBSAAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2373-Tier1-PhCMV-Gal4
Plasmid#169581PurposeTier-1 vector encoding PhCMV-driven Gal4 DNA binding domain (PhCMV-Gal4-pA).DepositorInsertregulatory protein GAL4
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2366-Tier1-PhCMV_F-type
Plasmid#169567PurposeTier-1 vector encoding PhCMV-driven F-type transactivator domain (PhCMV-F-type-pA).DepositorInsertPCMV-driven F-type VP16
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2392-Tier1-PhCMV-p2a_medFT
Plasmid#169529PurposeTier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "medium" maturation medFT fused to a "self-cleaving" peptide p2a (PhCMV-p2a_medFT-pA).DepositorInsertPCMV-driven P2A-fused fluorescent timer, medium maturation time
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2388-Tier1-PhCMV-p2a_Ypet
Plasmid#169525PurposeTier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet fused to a "self-cleaving" peptide p2a (PhCMV-p2a_YPet-pA).DepositorInsertPCMV-driven P2A-fused yellow fluorescent protein Ypet
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2387-Tier1-PhCMV-p2a_mTagBFP2
Plasmid#169524PurposeTier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 fused to a "self-cleaving" peptide p2a (PhCMV-p2a_mTagBFP2-pA).DepositorInsertPCMV-driven P2A-fused monomeric Tag blue fluorescent protein 2
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2389-Tier1-PhCMV-p2a_mRuby
Plasmid#169526PurposeTier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 fused to a "self-cleaving" peptide p2a (PhCMV-p2a_mRuby2-pA).DepositorInsertPCMV-driven P2A-fused enhanced yellow fluorescent protein
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1011-Tier1-PhCMV-Nluc
Plasmid#169545PurposeTier-1 vector encoding PhCMV-driven NLuc (PhCMV-NLuc-pA).DepositorInsertPCMV-driven NanoLuc Luciferase
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2385-Tier1-PhCMV-fastFT
Plasmid#169518PurposeTier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "fast" maturation fastFT (PhCMV-fastFT-pA).DepositorInsertPCMV-driven fluorescent timer, fast maturation time
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2384-Tier1-PhCMV-medFT
Plasmid#169517PurposeTier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "medium" maturation medFT (PhCMV-medFT-pA).DepositorInsertPCMV-driven fluorescent timer, medium maturation time
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2408-Tier1-PhCMV-TEVsite
Plasmid#169503PurposeTier-1 vector encoding PhCMV-controlled tabacco etch virus protease recognition site (PhCMV-TEVsite-pA).DepositorInsertPCMV-driven tobacco etch virus protease cleavage site
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2406-Tier1-PhCMV-NTEV
Plasmid#169501PurposeTier-1 vector encoding PhCMV-driven N-terminal fragment of a split tabacco etch virus protease splitTEV (PhCMV-N-TEV-pA).DepositorInsertPCMV-driven N-terminal fragment of split tobacco etch virus protease
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2405-Tier1-PhCMV-TEV
Plasmid#169500PurposeTier-1 vector encoding PhCMV-driven tabacco etch virus protease TEV (PhCMV-TEV-pA).DepositorInsertPCMV-driven tobacco etch virus protease
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2403-Tier1-PhCMV-ZeoR
Plasmid#169495PurposeTier-1 vector encoding PhCMV-driven zeocin resistance gene ZeoR (PhCMV-ZeoR-pA).DepositorInsertPCMV-driven Sh ble
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2402-Tier1-PhCMV-BlastR
Plasmid#169494PurposeTier-1 vector encoding PhCMV-driven blasticidin resistance gene BlastR (PhCMV-BlastR-pA).DepositorInsertPCMV-driven blasticidin-S deaminase
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2401-Tier1-PhCMV-HygroR
Plasmid#169493PurposeTier-1 vector encoding PhCMV-driven hygromycin resistance gene HygroR (PhCMV-HygroR-pA).DepositorInsertPCMV-driven hph
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1021-Tier1-PhCMV-PuroR
Plasmid#169492PurposeTier-1 vector encoding PhCMV-driven marker for puromycin selection (PhCMV-PuroR-pA)DepositorInsertPCMV-driven puromycin N-acetyl-transferase
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1018-Tier1-PhCMV-p2A
Plasmid#169491PurposeTier-1 vector encoding a self-cleaving peptides p2a sequence (PhCMV-p2A-pA).DepositorInsertPCMV-driven 2A sequence of porcine teschovirus-1
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only