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pLZRS-IRES-deltaNGFR

Plasmid

#72930

Purpose

retroviral expression with FACS-selectable marker (extracellular part of NGFR)

Depositor

Esther van de Vosse

Article

van_de_Vosse et al Blood. 2009 May 7;113(19):4548-55. doi:

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pAd PdxI-I-nGFP

Plasmid

#19411

Depositor

Douglas Melton

Article

Zhou et al Nature. 2008 Aug 27. ():.

Insert

pancreatic and duodenal homeobox 1 (Pdx1 Mouse)

Use

Adenoviral; Entry vector

Tags

GFP and IRES

Expression

Mutation

Promoter

Available since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only
pAd MafA-I-nGFP

Plasmid

#19412

Depositor

Douglas Melton

Article

Zhou et al Nature. 2008 Aug 27. ():.

Insert

v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian) (Mafa Mouse)

Use

Adenoviral; Entry vector

Tags

GFP and IRES

Expression

Mutation

Promoter

Available since

Nov. 12, 2008

Availability

Academic Institutions and Nonprofits only
pAd NeuroD-I-nGFP

Plasmid

#19414

Depositor

Douglas Melton

Article

Zhou et al Nature. 2008 Aug 27. ():.

Insert

neurogenic differentiation 1 (Neurod1 Mouse)

Use

Adenoviral; Entry vector

Tags

GFP and IRES

Expression

Mutation

Promoter

Available since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only
pAd 2B Ngn3-I-nGFP

Plasmid

#19410

Depositor

Douglas Melton

Article

Zhou et al Nature. 2008 Aug 27. ():.

Insert

neurogenin 3 (Neurog3 Mouse)

Use

Adenoviral; Entry vector

Tags

GFP and IRES

Expression

Mutation

Promoter

Available since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only
pLKO_V5.AU1.VSVg_NGFR

Plasmid

#158241

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

V5.AU1.VSVg

Expression

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-185-5p.sensor.PGK.dNGFR.WPRE

Plasmid

#85930

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-185-5p Sensor (MIR185 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

PGK

Available since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pBAD-pnGFP-Ultra

Plasmid

#157923

Purpose

Peroxynitrite sensor pnGFP-Ultra in E. coli (use with pEvol-BoF)

Depositor

Huiwang Ai

Article

Chen et al Cell Chem Biol. 2021 Jan 27. pii: S2451-

Insert

pnGFP-Ultra, a high performance peroxynitrite sensor

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-617.sensor.PGK.dNGFR.WPRE

Plasmid

#85921

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-617 Sensor (MIR617 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

PGK

Available since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO_V5.AU1.FLAG_NGFR

Plasmid

#158240

Purpose

Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

Depositor

Brian Brown

Article

Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

Insert

Pro-Code Tagged human dNGFR (NGFR Human)

Use

CRISPR and Lentiviral

Tags

V5.AU1.FLAG

Expression

Mutation

Truncation of the signaling domain

Promoter

EF1a

Available since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-639.sensor.PGK.dNGFR.WPRE

Plasmid

#85923

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-639 Sensor (MIR639 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

PGK

Available since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AB.pCCL.sin.cPPT.GFP.miR-210-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85920

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-210-3p Sensor (MIR210 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

PGK

Available since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 (+) NGFR-delta wt

Plasmid

#80020

Purpose

Mammalian expression of NGFR-minus cytoplasmic tail + KLGFFKR

Depositor

Chinten James Lim

Insert

NGFR (NGFR Human)

Use

Tags

KLGFFKR

Expression

Mammalian

Mutation

minus cytoplasmic tail + KLGFFKR

Promoter

CMV

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.GFP.miR-152-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85872

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-152-3p Sensor (MIR152 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

PGK

Available since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-370-5p.sensor.PGK.dNGFR.WPRE

Plasmid

#85854

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-370-5p Sensor (MIR370 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

PGK

Available since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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p70.unipA.NGFR.mCMV.PGK.mBFP2.p27K.WPRE

Plasmid

#228766

Purpose

insert a blue fluorescent protein mBFP2, a cyclin-dependent kinase inhibitor p27K

Depositor

Ferdinando Pucci

Article

Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

Insert

p27 kinase inhibitor (Cdkn1b Mouse)

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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p48.unipolyA.dNGFR.mCMV.PGK.eGFP.Gem1.WPRE

Plasmid

#226767

Purpose

vector with NGFR and GFP

Depositor

Ferdinando Pucci

Article

Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
P30.pA.NGFR-mSrtA.mCMV.hPGK.GFP.wpre

Plasmid

#226728

Purpose

backbone vector, GFP expression

Depositor

Ferdinando Pucci

Article

Hamilton et al Adv Biosyst. 2020 Dec;4(12):e2000007. do

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pXL_dSSN1_EF1a-tNGFR-2A-Puro

Plasmid

#200553

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 1

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN2_EF1a-tNGFR-2A-Puro

Plasmid

#201112

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 2

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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