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Plasmid#139219PurposemMoClo TUPV8, with CMV promoter and an MCSDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only
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pPD477 CMV MCS (TUPV7)
Plasmid#139218PurposemMoClo TUPV7, with CMV promoter and an MCSDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD476 CMV MCS (TUPV6)
Plasmid#139217PurposemMoClo TUPV6, with CMV promoter and an MCSDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD475 CMV MCS (TUPV5)
Plasmid#139216PurposemMoClo TUPV5, with CMV promoter and an MCSDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD474 CMV MCS (TUPV4)
Plasmid#139215PurposemMoClo TUPV4, with CMV promoter and an MCSDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD472 CMV MCS (TUPV2)
Plasmid#139213PurposemMoClo TUPV2, with CMV promoter and an MCSDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD471 CMV MCS (TUPV1)
Plasmid#139212PurposemMoClo TUPV1, with CMV promoter and an MCSDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV_RBMX-eA3A-UdgX-HF-NG-nCas9
Plasmid#163550PurposeMammalian CG-to-GC base editingDepositorInsertRBMX-eA3A-UdgX-HF-NG-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)N497A, R661A, Q695A, Q926A//L111R, D1…PromoterAvailable sinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_RKK/AAA POLI
Plasmid#131240PurposeMammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations R524A, K526A and K530ADepositorInsertDNA polymerase iota (POLI Human)
UseTagsFLAGExpressionMammalianMutationContains point mutations that convert arginine re…PromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_3KA + S580A POLI
Plasmid#131241PurposeMammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A, K446A and S580ADepositorInsertDNA polymerase iota (POLI Human)
UseTagsFLAGExpressionMammalianMutationContains point mutations that convert Pol iota se…PromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_1-590 POLI
Plasmid#131233PurposeMammalian expression of DNA polymerase iota residues 1-590 with an N-terminal FLAG-tagDepositorInsertDNA polymerase iota (POLI Human)
UseTagsFLAGExpressionMammalianMutationContains a 24 bp insertion within the Pol iota co…PromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_1-420 POLI
Plasmid#131234PurposeMammalian expression of DNA polymerase iota residues 1-420 with an N-terminal FLAG-tagDepositorInsertDNA polymerase iota (POLI Human)
UseTagsFLAGExpressionMammalianMutationContains a 24 bp insertion within the Pol iota co…PromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3x-FLAG-CMV-MSTN
Plasmid#157953Purposeexpression of MSTN geneDepositorInsertMSTN (MSTN Sus scrofa)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-TYK2VF/PA-VSV
Plasmid#139351PurposeExpression of the double mutant of TYK2-V678F-P1104ADepositorInsertTYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
UseTagsVSVExpressionMammalianMutationV362F-V678F-P1104APromoterCMVAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GFP-ILVBLpA-G10C
Plasmid#120206PurposeHuman ILVBL poly(A) site reporter, stem loop mutantDepositorInsertILVBL poly(A) site
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCMV-GFP-RPS5pA-del
Plasmid#120200Purposehuman RPS5 poly(A) site reporter, with stem loop deletedDepositorInsertRPS5 poly(A) site
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GFP-RPS5pA-m2r
Plasmid#120199PurposeHuman RPS5 poly(A) site reporter, stem loop mutant 2 rescueDepositorInsertRPS5 poly(A) site
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GFP-RPS5pA-m1r
Plasmid#120197PurposeHuman RPS5 poly(A) site reporter, stem loop mutant 1 rescueDepositorInsertRPS5 poly(A) site
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GFP-CENPBpA-del
Plasmid#120193Purposehuman CENPB poly(A) site reporter, with stem loop deletedDepositorInsertCENPB poly(A) site
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GFP-TFE3pA-del
Plasmid#120204Purposehuman TFE3 poly(A) site reporter, with stem loop deletedDepositorInsertTFE3 poly(A) site
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-GFP-TFE3pA-G9C
Plasmid#120202PurposeHuman TFE3 poly(A) site reporter, stem loop mutantDepositorInsertTFE3 poly(A) site
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-PnGW-CMV-Luc
Plasmid#133367PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLSDepositorInsertGFP-NLS
UseTagsHA-tagged GFPExpressionMammalianMutationN/APromoterCMVAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Flag-hcRed_IDG-K
Plasmid#135274PurposeGateway destination clone of hcRed (as control) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomicsDepositorInsertFlag-hcRed
UseLentiviral; Gateway destinationTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-ATG3 allKR
Plasmid#129298PurposeExpresses pCMV 3xFLAG-ATG3 allKR (all lysines mutated to arginine) in mammalian cellsDepositorInsertUbiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
UseTags3xFLAGExpressionMammalianMutationchanged all 22 Lysine residues to ArgininePromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-ATG3 K295R
Plasmid#129302PurposeExpresses pCMV 3xFLAG-ATG3 K295R in mammalian cellsDepositorInsertUbiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
UseTags3xFLAGExpressionMammalianMutationchanged Lysine 295 to ArgininePromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-GFP-shNts2
Plasmid#132714PurposeEncodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat NtsDepositorInsertshNts(2) (Nts Rat)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-GFP-shNts1
Plasmid#132713PurposeEncodes short hairpin RNA (shRNA) #1 that targets the the 3’-untranslated region of the rat Nts geneDepositorInsertshNts(1) (Nts Rat)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-GFP-shCrh2
Plasmid#132710PurposeEncodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Crh geneDepositorInsertshCrh(2) (Crh Rat)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceOct. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-TIRAP-cGASΔN-HA
Plasmid#130921PurposeExpresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the TIRAP N terminus (aa1-85); Puromycin selection markerDepositorInsertTIRAP-ΔNcGAS
UseTagsHAExpressionMammalianMutationcGAS N terminus (aa1-159) replaced with the N ter…PromoterCMVAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Fyn-cGASΔN-HA
Plasmid#130922PurposeExpresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the Fyn dual acylation motif (aa1-16); Puromycin selection markerDepositorInsertFyn-ΔNcGAS
UseTagsHAExpressionMammalianMutationcGAS N terminus (aa1-159) replaced with the dual …PromoterCMVAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (G20V)
Plasmid#119308Purposelentiviral vector for expression of HA-Tagged E6-AP I (G20V) in mammalian cellsDepositorInsertE6-AP I (G20V) (UBE3A Human)
UseLentiviralTagsHAExpressionMutationG20VPromoterCMVAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (C21Y)
Plasmid#119304Purposelentiviral vector for expression of HA-Tagged E6-AP I (C21Y) in mammalian cellsDepositorInsertE6-AP I (C21Y) (UBE3A Human)
UseLentiviralTagsHAExpressionMutationC21YPromoterCMVAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (AA 55-376)
Plasmid#119313Purposelentiviral vector for expression of HA-Tagged E6-AP I AA 55-376 in mammalian cellsDepositorInsertE6-AP I (UBE3A Human)
UseLentiviralTagsHAExpressionMutationOnly AA 55-376PromoterCMVAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (R39H)
Plasmid#119309Purposelentiviral vector for expression of HA-Tagged E6-AP I (R39H) in mammalian cellsDepositorInsertE6-AP I (R39H) (UBE3A Human)
UseLentiviralTagsHAExpressionMutationG20VPromoterCMVAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
IH401: pMVP (L5-L4) CMV promoter
Plasmid#121726PurposepMVP L5-L4 entry plasmid, contains CMV promoter for 4-component MultiSite Gateway Pro assemblyDepositorInsertCMV promoter
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA1063_pPB-hCMV*1-cHA-IRESH2BmKO2
Plasmid#124169PurposepiggyBac-based Dox-inducible expressionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMCh1750_S2F-IMCg_doxy-CMV_mChe-Cdc42E7
Plasmid#118614Purposeexpression of mChe-tagged cdc42E7 under doxy-inducible CMV promoterDepositorInsertcdc42E7 (Cdc42 Mouse)
UseLentiviralTagsmCherryExpressionMammalianMutationPromotertetON CMVAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
PHAGE-P CMVt N-HA hE6-AP I (AA 55-852)
Plasmid#119314Purposelentiviral vector for expression of HA-Tagged E6-AP I AA 55-852 in mammalian cellsDepositorInsertE6-AP I (UBE3A Human)
UseLentiviralTagsHAExpressionMutationOnly AA 55-852PromoterCMVAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJet-CMV-CD52-bglpA
Plasmid#89704PurposeHuman CD52 expression plasmid with short polyADepositorInsertCMV-CD52-bglpA (CD52 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2019AvailabilityAcademic Institutions and Nonprofits only