We narrowed to 15,981 results for: nes

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pDN41

Plasmid

#61344

Purpose

mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS9/PKIt NES)

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

Insert

mCherry

Tags

GS linker-AsLov2-biNLS9 and PKIt NES

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCI_Fsx1-V5_GFPnes

Plasmid

#240503

Purpose

Cell fusion assays

Depositor

Benjamin Podbilewicz

Article

Moi et al Nat Commun. 2022 Jul 6;13(1):3880. doi:

Insert

Fsx1

Tags

GFPnes and V5

Expression

Mammalian

Available Since

July 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCI_VSV-G_GFPnes

Plasmid

#240505

Purpose

Cell fusion assays

Depositor

Benjamin Podbilewicz

Article

Moi et al Nat Commun. 2022 Jul 6;13(1):3880. doi:

Insert

VSV-G

Tags

GFPnes

Expression

Mammalian

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCI_IZUMO1-V5_GFPnes

Plasmid

#240497

Purpose

Cell fusion assays

Depositor

Benjamin Podbilewicz

Article

Brukman et al J Cell Biol. 2023 Feb 6;222(2):e20220714

Insert

IZUMO1 (Izumo1 Mouse)

Tags

GFPnes and V5

Expression

Mammalian

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCI_EFF-1-V5_GFPnes

Plasmid

#240504

Purpose

Cell fusion assays

Depositor

Benjamin Podbilewicz

Article

Moi et al Nat Commun. 2022 Jul 6;13(1):3880. doi:

Insert

EFF-1

Tags

GFPnes and V5

Expression

Mammalian

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pHHM.X513.iS-methadoneSnFR

Plasmid

#220861

Purpose

Bacterial expression of iS-methadoneSnFR for purification and in vitro assays.

Depositor

Henry Lester

Article

Muthusamy et al J Am Chem Soc. 2022 May 18;144(19):8480-

Insert

iS-methadoneSnFR

Tags

His6-HA and Myc

Expression

Bacterial

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pEND-443_Cacnes_HisB

Plasmid

#225608

Purpose

Suicide C. acnes vector to generate HisB (PPA_RS05815) deletion leaving an Ery resistance cassette flanked by BxbI recombination sites

Depositor

Marc Güell

Article

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pEND-225_Cacnes_mKO2

Plasmid

#225612

Purpose

Replicative vector for orange fluorescent reporter protein mKO2 strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mKO2

Depositor

Marc Güell

Article

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Insert

mKO2

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Codon optimized for Cutibacterium acnes

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pHcRed-NPMMLF1nes-C1

Plasmid

#131849

Purpose

Expresses the N-terminal nuclear export signal of HcRed-NPMMLF1 in mammalian cells through transient transfection

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

NPM1 (NPM1 Human)

Tags

HcRed

Expression

Mammalian

Mutation

This is a control deletion contruct of the cytop…

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(2)_T2AGeneSwitch

Plasmid

#125216

Purpose

Artificial phase 2 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GeneSwitch

Use

Other

Tags

T2A

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(1)_T2AGeneSwitch

Plasmid

#125215

Purpose

Artificial phase 1 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GeneSwitch

Use

Other

Tags

T2A

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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mEos2-Farnesyl-5

Plasmid

#57375

Purpose

Localization: Membrane, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Catherine Galbraith

Article

Jaqaman et al Mol Biol Cell. 2016 May 15;27(10):1561-9

Insert

Farnesyl (HRAS Human)

Tags

mEos2

Expression

Mammalian

Mutation

encodes final 20 aa of NM_001130442.1

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pET-HA-MBP-CatN-TEV-CfaC-eGFP-FLAG

Plasmid

#241423

Purpose

Protein Transposition Cassette Containing CatN and CfaC

Depositor

Thomas Muir

Article

Hua et al Science. 2025 Apr 4;388(6742):68-74. doi

Insert

His5-Sumo-HA-MBP-CatN-TEV-CfaC-eGFP-FLAG

Tags

His5, HA

Expression

Bacterial

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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Cyto-B

Plasmid

#209858

Purpose

Dimerization dependent fluorescent protein B fused to NES

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddGFP B

Tags

NES sequence LALKLAGLDIGS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pDD3254 pCS2-NES-arrb2a-GGSGGSG-ENLYFQ/S-GGSGGS-SV40NLS-sfCherry3C(1-10)

Plasmid

#251769

Purpose

TEVp substrate (GS-flanked motif) for fluorescent protein reconstitution assay

Depositor

Dave Dingal

Article

Lim Suan et al Nat Commun 17, 3257 (2026).

Insert

arrestin, beta 2a (arrb2a Zebrafish)

Tags

NES and NLS (SV40), sfCherry3C large fragment

Expression

Mammalian

Promoter

CMV IE94

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pDD3248 pCS2-mtagBFP2-p2a-NES-arrb2a-(ENLYFQ/S)x3-SV40NLS-sfCherry3C(1-10)

Plasmid

#251770

Purpose

Blue fluorescent protein-tagged TEVp substrate (three-peat motif) for fluorescent protein reconstitution assay

Depositor

Dave Dingal

Article

Lim Suan et al Nat Commun 17, 3257 (2026).

Insert

arrestin, beta 2a (arrb2a Zebrafish)

Tags

NLS (SV40), sfCherry3C large fragment and mTagBFP…

Expression

Mammalian

Promoter

CMV IE94

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-NLS-msfGFP-T2A-mCherry-NES> mPGK-PuroR

Plasmid

#218916

Purpose

Lentiviral construct expressing msfGFP and nuclear mCherry proteins.

Depositor

Sven Eyckerman

Article

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

EF1a-msfGFP-T2A-mCherry-NLS > mPGK-PuroR

Use

Lentiviral

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ΔN-RNMT

Plasmid

#112708

Purpose

Expresses FLAG-NES-RNMT 121-476 D203A ("ΔN-RNMT") in mammalian cells. Overexpression inhibits cytoplasmic cap methylation.

Depositor

Daniel Schoenberg

Article

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

RNMT (RNMT Human)

Tags

FLAG and HIV Rev nuclear export signal (NES)

Expression

Mammalian

Mutation

deleted amino acids 2-120, mutated active-site D2…

Promoter

CMV

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-4493NES(Booster-PKA)

Plasmid

#184710

Purpose

PKA biosensor

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

Booster-PKA

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-gLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)

Plasmid

#125225

Purpose

Expresses NanoLuc-β2AR-AsLOV2(V416L) in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

gLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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