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pGAL4-DBD-NANOG-WT

Plasmid

#215599

Purpose

For overexpression of Gal4-DBD NANOG WT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

NANOG (NANOG Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-FOXA3_WT

Plasmid

#215639

Purpose

For overexpression of Gal4-DBD FOXA3 WT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

FOXA3 (FOXA3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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FUW-MDmut-Gata2

Plasmid

#193091

Purpose

constitutive expression of a mutated (mut, R42A), non-functional version of the mitosis degradation (MD) domain of cyclin B1 fused to mouse Gata2 in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

MDmut-Gata2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Ubc

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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sgVRK1-hygro

Plasmid

#199640

Purpose

sgRNA guide against VRK1

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A (VRK1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCHSU64.1-2.2

Plasmid

#115542

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

PKS

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pEJS592-pCSDest2-AcrIIC4Hpa-BFPv2-IRES

Plasmid

#113438

Purpose

Expresses type II-C anti-CRISPR protein from H. parainfluenzae and a BFP from IRES in mammalian cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Inserts

Type II-C anti-CRISPR
mTagBFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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sgGLDC_2

Plasmid

#72887

Purpose

CRISPR-Cas9 induced gene disruption

Depositor

David Sabatini

Article

Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

Insert

sgGLDC

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Feb. 4, 2016

Availability

Academic Institutions and Nonprofits only
sgGLDC_1

Plasmid

#72886

Purpose

CRISPR-Cas9 induced gene disruption

Depositor

David Sabatini

Article

Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

Insert

sgGLDC

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
Human Beta-catenin GFP

Plasmid

#71367

Purpose

mammalian expression of human Beta-catenin GFP

Depositor

Alpha Yap

Article

Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

Insert

Beta-catenin (CTNNB1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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Lentiviral-TOP-dGFP-reporter

Plasmid

#14715

Purpose

Beta-catenin reporter - 3rd generation self-inactivating lentiviral plasmid LEF-1/TCF-responsive promoter driven d2-eGFP (destabilized, half-life of 2h).

Depositor

Tannishtha Reya

Article

Reya et al Nature. 2003 May 22. 423(6938):409-14.

Insert

LEF-1/TCF-responsive promoter (Ctnnb1 )

Use

Lentiviral

Tags

d2 eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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prhaBAD-pA-Hia5

Plasmid

#222303

Purpose

Plasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

Depositor

Aaron Straight

Article

Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

Insert

pA-Hia5

Use

Tags

6His and protein A

Expression

Bacterial

Mutation

codon optimized for bacterial expression

Promoter

rhaBAD

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-FOXA3_AroLITE

Plasmid

#215640

Purpose

For overexpression of Gal4-DBD FOXA3 AroLITE

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

FOXA3 AroLITE (FOXA3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-FOXA3_AroPERFECT

Plasmid

#215641

Purpose

For overexpression of Gal4-DBD FOXA3 AroPERFECT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

FOXA3 AroPERFECT (FOXA3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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SUPERBLADE5

Plasmid

#134913

Purpose

Empty backbone for cloning sgRNA sequence to be used in nanoblades system (Optimized for increased genome editing efficiency via Chen B et al., 2013)

Depositor

Théophile Ohlmann, Emiliano Ricci, Philippe Mangeot

Article

Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pMP71-HLA-A0201-His

Plasmid

#108214

Purpose

HLA-A0201 his tagged

Depositor

Sébastien Walchli

Article

Lampen et al Immunohorizons. 2018 Jul 2;2(6):172-184.

Insert

major histocompatibility complex, class I, B (HLA-A Human)

Use

Retroviral

Tags

his

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pAS_4xG1 PylT FLAG-G1 PylRS Y125A

Plasmid

#154769

Purpose

plasmid with 4xG1 PylT cassette and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Y125A

Promoter

EF1

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-ArchT-tdTomato

Plasmid

#28305

Purpose

Cre-dependent viral expression of ArchT-tdTomato for neuronal inhibition

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Has Service

AAV5

Insert

ArchT-tdTomato

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

tdTomato

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-ArchT-GFP

Plasmid

#28307

Purpose

Cre-dependent viral expression of ArchT-GFP for neuronal inhibition

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Insert

ArchT-GFP

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

GFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

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SHMT2-RES

Plasmid

#72880

Purpose

overexpression

Depositor

David Sabatini

Article

Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

Insert

SHMT2 (SHMT2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

silent mutations in site corresponding to shSHMT2…

Promoter

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pSIN4-EF1a-GATA2-IRES-Puro

Plasmid

#61063

Purpose

lentiviral vector for GATA2

Depositor

Igor Slukvin

Article

Elcheva et al Nat Commun. 2014 Jul 14;5:4372. doi: 10.

Insert

GATA2 (GATA2 Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

EF1alpha

Available since

March 27, 2015

Availability

Academic Institutions and Nonprofits only

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