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Plasmid#123130PurposeBacterial expression plasmid for wild-type Dre with an N-terminal His-tag.DepositorInsertDre recombinase
UseTags6x His tagExpressionBacterialMutationPromoterT7Available sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM-30-GST-PfAgo_F
Plasmid#146265PurposeBacterial Expression of PfAgoDepositorInsertPfAgo
UseTagsExpressionBacterialMutationone silent T1704C and one non silent mutation A90…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pet42c(+)-GST-USP13
Plasmid#61749Purposeexpress N-terminally GST tagged human USP13DepositorInsertUSP13 (USP13 Human)
UseTagsGSTExpressionBacterialMutationPromoterT7Available sinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-mCherry
Plasmid#109421PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCherry. VHH-mCherry also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, mCherry, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, and T7ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-HIS-Sangamo
Plasmid#40786Purposefor e. coli expression, Golden Gate Compatible TALEN incorporated with N Terminal 6xHIS Tag, Codon Optimized FokIDepositorInsertTranscription Activator Like Effector Nuclease
UseTALENTagsN Terminal 6xHISExpressionBacterialMutation+63 C Terminus, Codon Optimized FokI EndonucleasePromoterT7Available sinceDec. 12, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET21b Apaf1 1-591
Plasmid#26642DepositorInsertApoptotic protease activating factor 1 (APAF1 Human)
UseTagsHisExpressionBacterialMutationAmino Acids 1-591PromoterAvailable sinceNov. 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+18)
Plasmid#199162Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+18)
UseTagsMGHHHHHHGGAExpressionMutationE16R, D29K, E42K, E44K, D86K, D112K, T115K, D127R…PromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-Sac-Aβ(M1-42/C18C33)
Plasmid#173759PurposeExpresses the AβC18C33 peptide.DepositorInsertAβ(M1-42/C18C33) (APP Human)
UseTagsExpressionBacterialMutationchanged valine 18 to cysteine; changed glycine 33…PromoterT7Available sinceAug. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28b-HRASLS3-1-132
Plasmid#100725PurposeExpression of N-terminal region of human HRASLS3 in E.coliDepositorInsertHRASLS3 (PLAAT3 Human)
UseTagsHisExpressionBacterialMutationPromoterT7 promotorAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-Ran(Q69L)
Plasmid#42048DepositorInsertRan (RAN Human)
UseTagsHisExpressionBacterialMutationQ69LPromoterAvailable sinceFeb. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pet42c(+)-GST-USP5
Plasmid#61750Purposeexpress N-terminally GST tagged human USP5DepositorInsertUSP5 (USP5 Human)
UseTagsGSTExpressionBacterialMutationPromoterT7Available sinceFeb. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-Sangamo-HIS
Plasmid#40787Purposefor e. coli expression, Golden Gate Compatible TALEN incorporated with C Terminal 6xHIS Tag, Codon Optimized FokIDepositorInsertTranscription Activator Like Effector Nuclease
UseTALENTagsC Terminal 6xHISExpressionBacterialMutation+63 C Terminus,Codon Optimized FokI EndonucleasePromoterT7Available sinceDec. 12, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-Bst-LF-Idaho
Plasmid#170469PurposeExpresses His-tagged Bst-LF-Idaho in E. coliDepositorInsertBst-LF-Idaho
UseTagsN-terminal His-tagExpressionBacterialMutationPromoterAvailable sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETDuet_sfCherry2(1-10)_sfCherry2(11)-SpyCatcher
Plasmid#117656PurposeBacterial expression vector for split sfCherry3 screening. The “sfCherry2(1-10)” and “sfCherry2(11)-SpyCatcher002” are expressed from two different T7 promoters.DepositorInsertssfCherry2(1-10)
sfCherry2(11)-SpyCatcher002
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(0)
Plasmid#199166Purposeused to express a neutral GFP variant in E. coliDepositorInsertGFP(0)
UseTagsMGHHHHHHGGAExpressionMutationE16R, I138R, D207R, N222K, L246RPromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-APOBEC1-YTH
Plasmid#200158PurposeFor bacterial expression of the APOBEC1-YTH fusion protein for protein purificationDepositorInsertAPOBEC1-YTH
UseTags6xHis, HA, and Maltose Binding ProteinExpressionBacterialMutationCodon optimized for E coli expressionPromoterLacZAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Ec.coaD (pESC106)
Plasmid#50388PurposeHeterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.DepositorInsertPPAT
UseTags6xHis-tagExpressionBacterialMutationPromoterT7Available sinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21a-Core Traptavidin
Plasmid#26054DepositorInsertCore Traptavidin
UseTagsExpressionBacterialMutationStreptavidin with 2 amino acid mutations: S52G R5…PromoterAvailable sinceAug. 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pETSXM2-pLacI-GFP
Plasmid#174617PurposeBacterial expression of GFP.DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLacIAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-HIS-Tol2
Plasmid#112486Purposefunctional recombinant Tol2 protein from E.coliDepositorInsertTol2 Transposase (tpase Synthetic)
UseTagsHIS/6xExpressionBacterialMutationPromoterT7Available sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only