We narrowed to 2,369 results for: puc19

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pMT1420

Plasmid

#228316

Purpose

Homoserine lactone (HSL)-regulatable EGFP expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

EGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

Homoserine lactone (HSL)-regulatable synthetic pr…

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pMT950

Plasmid

#228290

Purpose

2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

EGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

2,4-Diacetylphloroglucinol-regulatable synthetic …

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pP2A_NLS_sfGFP

Plasmid

#63709

Purpose

Expressing P2A_NLS_sfGFP with homologous arm of Nanog.

Depositor

Stanley Qi

Article

Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.

Insert

Nanog homologous arm, p2A, NLS, sfGFP, Nanog homologous arm

Use

Just a donor dna with nanog homologous arm for kn…

Tags

sfGFP

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_hPGK1_mScarlet-I-TOSI_Donor

Plasmid

#178087

Purpose

HDR donor plasmid to introduce the mScarlet-I mTOR signaling indicator (TOSI) cassette to AAVS1 using AAVS1 T2 sgRNA.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

mScarlet-I mTOR signaling indicator (TOSI)

Use

CRISPR

Expression

Mammalian

Promoter

Human PGK1

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pJBL728

Plasmid

#140398

Purpose

Expresses CsoR-regulated 3WJdB RNA aptamer driven by T7 promoter with the csoO sequence 5BP downstream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-csoO-3WJdB-T

Use

Synthetic Biology

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL716

Plasmid

#140386

Purpose

Expresses MphR-regulated 3WJdB RNA aptamer driven by T7 promoter with the mphO sequence 5BP downstream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-mphO-3WJdB-T

Use

Synthetic Biology

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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HBT-GCaMP6-HA

Plasmid

#107433

Purpose

For Arabidopsis mesophyll protoplast transient expression assay

Depositor

Jen Sheen

Article

Liu et al Nature. 2017 May 18;545(7654):311-316. d

Insert

GCaMP6S

Tags

HA

Expression

Bacterial

Available Since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pAT15547_PEmax-SuperFi-Cas9

Plasmid

#184377

Purpose

Mammalian expression of SuperFi-Cas9 PEmax prime editor

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

Insert

PEmax-SuperFi-Cas9

Use

CRISPR

Tags

SV40 NLS and c-myc NLS

Expression

Mammalian

Mutation

H840A, R221K, N394K, Y1010D, Y1013D, Y1016D, V101…

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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Empty Target Control

Plasmid

#127667

Purpose

Encodes for the CRY2-HA and mCherry proteins expressed from the EF1a promoter

Depositor

Jennifer Phillips-Cremins

Article

Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

Insert

CRY2-HA-2A-mCherry

Tags

HA

Expression

Mammalian

Promoter

EF1a

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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qTAG-C-Solo-mStayGold-PXN

Plasmid

#227316

Purpose

Donor template for mStayGold insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

PXN Homology Arms flanking a mStayGold Tag (PXN Human)

Use

CRISPR; Donor template

Expression

Mammalian

Promoter

Promoterless

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-C-Solo-mScarlet-H2BC11

Plasmid

#207756

Purpose

Donor template for mScarlet insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

H2BC11 Homology Arms flanking a mScarlet Tag (H2BC11 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Nov. 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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KTK_354

Plasmid

#180525

Purpose

KTK compatible Intergration/suicide plasmid. Contains a LacZ dropout cassette flanked by regions homolgous to the arsenic resistance cassete.

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pJB332

Plasmid

#115949

Purpose

ORF insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 TetR-Gly9-DDX6 (contains SapI)

Use

Synthetic Biology

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pTW122

Plasmid

#115962

Purpose

ORF insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 5'-2xTetApt-mVenus-PEST

Use

Synthetic Biology

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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3xFlag-HaloTag-EZH2 HDR - pDY053

Plasmid

#171108

Purpose

Homology directed repair plasmid for generating HaloTag-EZH2 cells

Depositor

Thomas Cech

Article

Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

Insert

EZH2 (EZH2 Human)

Tags

HaloTag

Expression

Mammalian

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pJBL724

Plasmid

#140394

Purpose

Expresses HucR-regulated 3WJdB RNA aptamer driven by T7 promoter with the hucO sequence 5BP downstream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-hucO-3WJdB-T

Use

Synthetic Biology

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL726

Plasmid

#140396

Purpose

Expresses SmtB-regulated 3WJdB RNA aptamer driven by T7 promoter with the smtO sequence 5BP downstream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-smtO-3WJdB-T

Use

Synthetic Biology

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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TRAC_DARIC(2-3)_CD22-BBz_AAV6_Donor

Plasmid

#211906

Purpose

Vector for AAV6 donor production. Targeted CD22-BBz DARIC transgene integration to the TRAC locus via homology-directed repair.

Depositor

Yannick Doyon

Article

Levesque et al bioRxiv 2023.09.14.557485

Insert

DARIC-CD22-BBz

Use

AAV

Expression

Mammalian

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pRP(Exp)-CMV> ORF_924bp (Azgp1):T2A:dTomato:IRES:Puro

Plasmid

#110830

Purpose

Expresses zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter where both sequences are separated by a T2A self-cleaving peptide sequence as a result, the dT is not fused with the ZAG protein.

Depositor

Ande Satyanarayana

Article

Elattar et al FASEB J. 2018 Mar 23:fj201701465RR. doi:

Insert

AZGP1 (Azgp1 Mouse)

Use

Non-viral gene expression vector

Tags

T2A and dTomato

Promoter

CMV

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pHS1528

Plasmid

#205982

Purpose

U6-MWEE01000013 Cas5-HNH DR-BpiI-DR

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas5-HNH DR-BpiI-DR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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