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pLenti_CMV:Flag-Antares

Plasmid

#208852

Purpose

Expresses FLAG epitope-tagged Antares "Orange" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

Antares

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-H2B-pr-mEosFP

Plasmid

#141101

Purpose

H2B fused to pr-mEosFP for chromatin visualization and primed conversion

Depositor

Periklis Pantazis

Article

Welling et al Bio Protoc. 2020 Jun 5;10(11):e3645. doi

Insert

Histone 2B

Tags

pr-mEosFP

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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CRY2olig-PR

Plasmid

#218917

Purpose

Expression of CRY2olig-mCherry fused to a codon-optimised poly-PR sequence

Depositor

Tatyana Shelkovnikova

Article

Hodgson et al iScience. 2024 Sep 14;27(10):110937. doi

Insert

CRY2olig-mCherry tagged poly-PR

Expression

Mammalian

Mutation

wild-type

Promoter

CMV

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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CRY2olig-GR

Plasmid

#218919

Purpose

Expression of CRY2olig-mCherry fused to a codon-optimised poly-GR sequence

Depositor

Tatyana Shelkovnikova

Article

Hodgson et al iScience. 2024 Sep 14;27(10):110937. doi

Insert

CRY2olig-mCherry tagged poly-GR

Expression

Mammalian

Mutation

wild-type

Promoter

CMV

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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LLP791_PB_dCas9-SSSavi_BirA

Plasmid

#211786

Purpose

PiggyBac plasmid with dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), and BirA, with Hygro selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

dCas9, Spy, Snoop, aGCN4, AviTags

Tags

3xHA

Expression

Mammalian

Mutation

dCas9 D10A and H840A

Promoter

pCAGG abd pEF1a

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGCP123-EbpA_g1

Plasmid

#153517

Purpose

sgRNA for ebpA gene (NT) under nisin-inducible nisA promoter, barcoded

Depositor

Kimberly Kline

Article

Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

Insert

pnisA-sgRNA(EbpA_g1)-dCas9 scaffold

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-AID

Plasmid

#61926

Purpose

Expression of human AID tagged with EGFP

Depositor

Jon Houseley

Article

Lambert et al PLoS One. 2013 Dec 4;8(12):e82110. doi:

Insert

AICDA (AICDA Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pCR1063

Plasmid

#111091

Purpose

Expresses dCas9-KRAB tagged with mCherry: pHR-hEF1a-dCas9-mcherry-KRAB-mPGK-Hyg

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

dCas9-KRAB

Use

Lentiviral

Tags

mCherry

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-YeNL

Plasmid

#208849

Purpose

Expresses FLAG epitope-tagged YeNL "Yellow" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

YeNL Nanolantern

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-OeNL

Plasmid

#208850

Purpose

Expresses FLAG epitope-tagged OeNL "Orange" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

OeNL Nanolantern

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-GpNLuc

Plasmid

#208840

Purpose

Expresses FLAG epitope-tagged GpNLuc "Green" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

GpNLuc LumiFluor

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-CeNL

Plasmid

#208839

Purpose

Expresses FLAG epitope-tagged CeNL "Blue" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

CeNL Nanolantern

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-sbGLuc-B7-EYFP

Plasmid

#114110

Purpose

fusion protein of Gaussia luciferase variant slow burn, B7 transmembrane region, and enhanced yellow fluorescent protein; control for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Gomez-Ramirez et al J Neurosci Res. 2020 Mar;98(3):471-480.

Inserts

Gaussia luciferase, slow burn
B7 hinge region
Enhanced Yellow Fluorescent Protein

Use

AAV

Expression

Mammalian

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-NRCH-PE2-BSD

Plasmid

#191101

Purpose

Lentiviral expression plasmid of NRCH-PE prime editor with P2A-BSD marker

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

NRCH-PE2-BSD

Use

CRISPR and Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF-1a

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-UnaG-P2A-FusionRed

Plasmid

#191109

Purpose

Mammalian cell line expression plasmids for production of UnaG and FusionRed proteins

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

UnaG

Use

AAV

Tags

FusionRed

Expression

Mammalian

Mutation

See depositor comments below

Promoter

chicken β-actin promoter

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV7.1_3xFLAG-WWC2

Plasmid

#172995

Purpose

constitutive expression of FLAG-tagged WWC2

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

3xFLAG-WWC2 (WWC2 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGCP123-GFP_g1

Plasmid

#153518

Purpose

sgRNA to GFP gene (NT) under nisin-inducible nisA promoter, barcoded

Depositor

Kimberly Kline

Article

Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

Insert

PnisA-sgRNA(GFP_g1)_dCas9 scaffold (barcoded)

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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GDAP2-c005

Plasmid

#173110

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

GDAP2 (GDAP2 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

M1-N231

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-OgNLuc

Plasmid

#208851

Purpose

Expresses FLAG epitope-tagged OgNLuc "Orange" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

OgNLuc LumiFluor

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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k3(long) luciferase

Plasmid

#11110

Depositor

Anjana Rao

Article

Tsai et al Mol Cell Biol. 1996 Feb . 16(2):459-67.

Insert

k3(L) (TNF Human)

Tags

luciferase

Expression

Bacterial

Available Since

April 24, 2006

Availability

Academic Institutions and Nonprofits only