We narrowed to 24,649 results for: fer
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Plasmid#163654PurposeExpresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail from TFR and TMD and luminal domain from ST6GAL1 in mammalian cellsDepositorInsertUseTagsGFPExpressionMammalianMutationThis chimera contains the cytosolic tail from TFR…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only
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SSF-GFP
Plasmid#163656PurposeExpresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail and TMD from ST6GAL1, and luminal domain from TFR in mammalian cellsDepositorInsertUseTagsGFPExpressionMammalianMutationThis chimera contains the cytosolic tail from ST,…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pF711-pET28a-Hs-delta25-MCAD-NHis wt
Plasmid#85112Purposeexpression of recombinant human mature (delta25) MCAD wt with N-terminal 6xHis-tagDepositorInserthuman mature (delta25) MCAD (ACADM Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pF714-pET28a-Hs-FL-METTL20-NHis wt
Plasmid#85115Purposeexpression of recombinant human full-length METTL20 wt with N-terminal 6xHis-tagDepositorInserthuman full-length METTL20 (METTL20 Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
FFS-GFP
Plasmid#163659PurposeExpresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail and TMD from TFR, and luminal domain from ST6GAL1 in mammalian cellsDepositorInsertUseTagsGFPExpressionMammalianMutationThis chimera contains the cytosolic tail from TFR…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
SFF-GFP
Plasmid#163657PurposeExpresses a TFR and ST6GAL1 swapped chimera with the cytosolic tail from ST6GAL1, and TMD and luminal domain from TFR in mammalian cellsDepositorInsertUseTagsGFPExpressionMammalianMutationThis chimera contains the cytosolic tail from ST,…PromoterAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-LMO4 (GLucM23-VChR1-EYFP)
Plasmid#114101Purposefusion protein of Gaussia luciferase variant M23, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertGaussia luciferase, M23; Volvox Channelrhodopsin 1; EYFP
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry_CEACAM1
Plasmid#140102PurposeCRISPR-Cas9 library validationDepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralTagsExpressionMutationPromotergRNA1 under U6 and gRNA2 under H1Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-CMV2 MAML LHHLL
Plasmid#87681PurposeMammalian expression vector for Maml1 with LHHLL mutationDepositorInsertMAML1 (MAML1 Human)
UseTagsFlagExpressionMammalianMutationMutate 1010,1011 from DD to HHPromoterCMVAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-SERINC2-202
Plasmid#117364PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR SERINC2-202
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1Available sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry_Rat_cebp-alfa
Plasmid#140098PurposeCRISPR-Cas9 library validation (positive control)DepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralTagsExpressionMutationPromotergRNA1 under U6 and gRNA2 under H1Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry_SPI1
Plasmid#140099PurposeCRISPR-Cas9 library validation (positive control)DepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralTagsExpressionMutationPromotergRNA1 under U6 and gRNA2 under H1Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSAM2_mCherry_Tbx5
Plasmid#72688PurposeDoxycyline regulated expression of Tbx5 in mammalian cellsDepositorInsertTbx 5 (Tbx5 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterCMV-DoxycyclineAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL-p21UTRm1
Plasmid#20878DepositorInsertp21 3'UTR site 1 mutation (Cdkn1a Mouse)
UseLuciferaseTagsExpressionMammalianMutationPredicted miRNA binding site 1 (Position ~420) GC…PromoterAvailable sinceAug. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
sTR056
Plasmid#177369PurposeToehold switch, with an unpaired toehold region at the 5′-end that interacts with the trigger RNA (sTR060, Addgene plasmid # 177370) to unfold the switch.DepositorInsertsfGFP
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
sTR060
Plasmid#177370PurposeTrigger RNA (GCAGGGATAAACGAGATAGATAAGATAAGA) that pairs with the toehold region in sTR056 (Addgene plasmid #177369) to restore translational activity.DepositorInsertTrigger RNA
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO-BAX-201
Plasmid#117332PurposeDual luciferase assay for miRNA-targeted UTRDepositorInsert3' UTR BAX-201
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterMurine PGK-1Available sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBullet-pm-c
Plasmid#53078Purposedestination vector with PIP2a-ECFP (plasma membrane marker) for tagged fluorescent protein (C-ter-EYFP) of plant geneDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-SFFV-GFP-RELA K5R
Plasmid#196631PurposeExpress GFP-RELA K5RDepositorInsertGFP-RELA K5R (RELA Human)
UseLentiviralTagsGFPExpressionMutationK122R, K123R, K310R, K314R, K315RPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.10 Dub3
Plasmid#72684Purposeluciferase reporter with Dub3 promoterDepositorInsertDub3 promoter (Usp17le Mouse)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterDub3Available sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only