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mEmerald-MAP4-C-10

Plasmid

#54152

Purpose

Localization: Microtubules, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

Insert

MAP4

Tags

mEmerald

Expression

Mammalian

Mutation

aa 640-1045 of MAP4

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pMC-6-GFP-P2A-NptII-9

Plasmid

#197741

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

EGFP-P2A-NptII bicistronic CDS

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMO746

Plasmid

#117480

Purpose

upp in an artificial operon with npt from pMO9071 and AmpR-pUC ori from pCR4/TOPO, Pnpt-npt-upp;, KanR

Depositor

Judy Wall

Article

Parks et al Science. 2013 Mar 15;339(6125):1332-5. d

Insert

uracil phosphoribosyltransferase

Expression

Bacterial

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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PERK 3: PERK.dC.9E10.pCDNA

Plasmid

#21816

Depositor

David Ron

Article

Harding et al Nature. 1999 Jan 21. 397(6716):271-4.

Insert

PERK (C-term deleted) (Eif2ak3 Mouse)

Tags

Myc (9E10)

Expression

Mammalian

Mutation

amino-acids 582-1081 deleted

Available Since

July 27, 2010

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 gp78fl

Plasmid

#37375

Depositor

Allan Weissman

Article

Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

Insert

gp78 (AMFR Human)

Tags

EYFP

Expression

Mammalian

Mutation

P179L and F262L

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pDest-303

Plasmid

#159678

Purpose

Gateway destination vector for mammalian protein expression

Depositor

Dominic Esposito

Article

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pLG1-puro Non-targeting sgRNA 3

Plasmid

#109003

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

Non-targeting sgRNA #3

Use

Lentiviral

Expression

Mammalian

Promoter

mU6 promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP9A(E195Q)-HA

Plasmid

#209244

Purpose

Mammalian expression of ATP9A mutant E195Q

Depositor

Hye-Won Shin

Article

Tone et al FEBS Lett. 2020 Feb;594(3):412-423. doi:

Insert

ATP9A (ATP9A Human)

Tags

HA

Expression

Mammalian

Mutation

E195Q

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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CAV1-K*R-HA

Plasmid

#27765

Depositor

Ari Helenius

Article

Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

Insert

caveolin-1, lysine null mutant (CAV1 Human)

Tags

HA

Expression

Mammalian

Mutation

All lysines mutated to arginines (K*R)

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pMTH PKC alpha

Plasmid

#8409

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Mouse Protein Kinase C alpha (Prkca Mouse)

Expression

Mammalian

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
mNeonGreen-IETD-NanoLuc

Plasmid

#98288

Purpose

caspase-8 BRET Sensor

Depositor

Maarten Merkx

Article

den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

Insert

mNeonGreen-LEHD-NanoLuc

Tags

His-tag

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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PERK 4: PERKKD-pGEX4T-1

Plasmid

#21817

Depositor

David Ron

Article

Harding et al Nature. 1999 Jan 21. 397(6716):271-4.

Insert

PERK (537-1114) (Eif2ak3 Mouse)

Tags

GST and Myc (9E10)

Expression

Bacterial

Mutation

PERK Kinase Domain. Amino acids 537-1114.

Available Since

March 29, 2010

Availability

Academic Institutions and Nonprofits only

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pMuLE_ENTR_TOP-NLuc1.1_L5-L4

Plasmid

#113867

Purpose

Single pathway reporter; wnt promoter-driven expression of intracellular NanoLuc; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

TOP (wnt dependent promoter)
NLuc

Use

Gateway Cloning

Expression

Mammalian

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2-Lifeact-mRuby

Plasmid

#194339

Purpose

Expresses Lifeact with mRuby which labels F-actin in mammalian cells and zebrafish embryos

Depositor

Heidi Hehnly

Article

Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

Insert

Lifeact

Tags

mRuby

Expression

Mammalian

Promoter

CMV IE94

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-GeNL

Plasmid

#208841

Purpose

Expresses FLAG epitope-tagged GeNL "Green" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

GeNL Nanolantern

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB_mcherry

Plasmid

#167878

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only
GB4542

Plasmid

#215261

Purpose

Module for the expression of the N. nambi HISPS gene under the regulation of the copper inducible CBS4x:mDFR promoter and TNOS and the constitutve expression of CPH, LUZ, H3H, EGFP and p19

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

CBS4x:mDFR:HISPS-SF-CPH-SF-LUZ-H3H-EGFP-p19

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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GB4401

Plasmid

#215252

Purpose

Module for the expression of the N. nambi HISPS gene under the regulation of PNOS and TNOS and the constitutive expression of CPH, LUZ, H3H, EGFP and p19.

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

SF-PNOS:HISPS:TNOS-CPH-LUZ-H3H-EGFP-p19

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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CAV1-mEGFP-mCherry

Plasmid

#27763

Depositor

Ari Helenius

Article

Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

Insert

caveolin-1 (CAV1 Canis lupus familiaris)

Tags

monomeric EGFP-monomeric Cherry (tandem)

Expression

Mammalian

Available Since

July 11, 2011

Availability

Academic Institutions and Nonprofits only

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SAC1C389SdeltaTMD-FKBP-mCherry

Plasmid

#108124

Purpose

Recruitable cytosolic inactive SAC1

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

SACM1L(C389S; 1-520):FKBP1A(3-108):mCherry (SACM1L Human)

Expression

Mammalian

Mutation

SACM1L(C389S; 1-520)

Promoter

CMV

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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