We narrowed to 2,015 results for: D10

Showing: 1861 - 1880 of 2015 results

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pCMV_RBMX-eA3A-UdgX-HF-NG-nCas9

Plasmid

#163550

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

RBMX-eA3A-UdgX-HF-NG-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)N497A, R661A, Q695A, Q926A//L111R, D1…

Available Since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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T7pr-His6-MBP-TEV-Cas9-BirA*

Plasmid

#159993

Purpose

Bacterial expression of Cas9-BirA*

Depositor

Jacob Corn

Article

Wang et al Mol Cell. 2020 Jul 16;79(2):221-233.e5.

Insert

Cas9-BirA*

Tags

6X-His, MBP, 3X-FLAG, NLS

Expression

Bacterial

Mutation

D10A, H840A

Promoter

T7

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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E1b-GFP-Tol2-Gateway dre SE-irf2bpl C

Plasmid

#89372

Purpose

Vector for reporter assay of one zebrafish super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

irf2bpl (irf2bpl Zebrafish)

Available Since

June 15, 2017

Availability

Academic Institutions and Nonprofits only

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E1b-GFP-Tol2-Gateway dre SE-irf2bpl B

Plasmid

#89371

Purpose

Vector for reporter assay of one zebrafish super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

irf2bpl (irf2bpl Zebrafish)

Available Since

June 15, 2017

Availability

Academic Institutions and Nonprofits only

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E1b-GFP-Tol2-Gateway dre SE-irf2bpl D

Plasmid

#89373

Purpose

Vector for reporter assay of one zebrafish super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

irf2bpl (irf2bpl Zebrafish)

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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E1b-GFP-Tol2-Gateway dre SE-irf2bpl A

Plasmid

#89370

Purpose

Vector for reporter assay of one zebrafish super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

irf2bpl (irf2bpl Zebrafish)

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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p_sc-eVIP-delta-Puro-EGFP-NFKB2

Plasmid

#249467

Purpose

Transfer plasmid for stable transgene expression with 2nd or 3rd generation lentiviral systems

Depositor

Nils Blüthgen

Article

Hollek et al Mol Syst Biol. 2026 Jan 19. doi: 10.1038

Insert

NFKB2 (NFKB2 Human)

Use

Lentiviral

Promoter

EF-1a

Available Since

June 16, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-CRY2-STIM1(238-685)

Plasmid

#248293

Purpose

An expression construct encoding a CRY2-fused FLAG-tagged STIM1 fragment (a.a.238–685).

Depositor

Sangkyu Lee

Article

Kook et al Mol Brain. 2023 Oct 17;16(1):73. doi: 10

Insert

CRY2-fused FLAG-tagged STIM1 fragment (a.a.238–685) (STIM1 )

Tags

FLAG

Mutation

deleted amino acids 1-237

Promoter

CMV

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-EGFP-CRY2-STIM1(318-450)

Plasmid

#248294

Purpose

An expression construct encoding a CRY2-fused truncated STIM1 fragment (a.a.318–450).

Depositor

Sangkyu Lee

Article

Kook et al Mol Brain. 2023 Oct 17;16(1):73. doi: 10

Insert

CRY2-fused truncated STIM1 fragment (a.a.318–450) (STIM1 )

Tags

EGFP

Mutation

deleted amino acids 1-317,451-685

Promoter

CMV

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pGFP-mTet1-delCys pc3903

Plasmid

#201701

Purpose

Expresses of GFP-tagged mTet1-delCys in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

Tet1 (Tet1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Deletion of the Cysteine rich domain of Tet1

Promoter

CAG

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGFP-mTet1s-delCys pc3904

Plasmid

#201702

Purpose

Expresses of GFP-tagged mTet1s-delCys in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

Tet1s (Tet1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

Deletion of the Cysteine rich domain of Tet1s

Promoter

CAG

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-InPTapp

Plasmid

#236000

Purpose

Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4)P2. Targeted to lysosome surface.

Depositor

Jin Zhang

Article

Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

Insert

Lyso-InPTapp (PLEKHA1, LAMP1 Synthetic, Human)

Tags

Citrine, Full-length LAMP1, and truncECFP

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 solvvol

Plasmid

#232976

Purpose

Galactose iduced expression of Gcn4 solvvol in yeast

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 solvvol

Tags

TEV cleavage site

Expression

Yeast

Mutation

S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

Promoter

Gal1

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-LAMP1-Y414A

Plasmid

#222319

Purpose

Synchronize the trafficking of LAMP1-Y414A from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAMP1-Y414A fused to SBP-EGFP (LAMP1 Human)

Expression

Mammalian

Mutation

Tyr414 to Ala

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pSCBE3-HF1

Plasmid

#218156

Purpose

This plasmid harbors the base editor SCBE3-HF1 along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.

Depositor

Yuhui Sun

Article

Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

Inserts

scbe3-HF1
a programmable sgRNA cloning cassette

Use

CRISPR; Sgrna transcription

Expression

Bacterial

Mutation

Mutations in the portion of SpCas9 : D10A / N497A…

Promoter

rpsL promoter

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSCBE3-Hypa

Plasmid

#218157

Purpose

This plasmid harbors the base editor SCBE3-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.

Depositor

Yuhui Sun

Article

Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

Inserts

scbe3-Hypa
a programmable sgRNA cloning cassette

Use

CRISPR; Sgrna transcription

Expression

Bacterial

Mutation

Mutations in the portion of SpCas9 : D10A / N692A…

Promoter

rpsL promoter

Available Since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-ICAM2-Fc(DAPA)-AviTag-6xHis

Plasmid

#156610

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

ICAM2 (ICAM2 Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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p_sc-eVIP-delta-Puro-EGFP-NFKB2-R52Q

Plasmid

#249468

Purpose

Transfer plasmid for stable transgene expression with 2nd or 3rd generation lentiviral systems

Depositor

Nils Blüthgen

Article

Hollek et al Mol Syst Biol. 2026 Jan 19. doi: 10.1038

Insert

NFKB2 (NFKB2 Human)

Use

Lentiviral

Mutation

R52Q

Promoter

EF-1a

Available Since

June 16, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-CIBN-STIM1(238-685)

Plasmid

#248296

Purpose

An expression construct encoding a CIBN-fused STIM1 fragment (a.a.238-685).

Depositor

Sangkyu Lee

Article

Kook et al Mol Brain. 2023 Oct 17;16(1):73. doi: 10

Insert

CIBN-fused STIM1 fragment (a.a.238-685) (STIM1 Human)

Tags

CIBN

Expression

Mammalian

Mutation

deleted amino acids 1-237

Promoter

CMV

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-EGFP-IRES2-CRY2-STIM1(238-448)

Plasmid

#248295

Purpose

An expression construct encoding a CRY2-fused STIM1 fragment (a.a.238-448) linked to EGFP through an IRES2 element.

Depositor

Sangkyu Lee

Article

Kook et al Mol Brain. 2023 Oct 17;16(1):73. doi: 10

Insert

EGFP and CRY2-fused STIM1 fragment (a.a.238-448) (STIM1 )

Tags

EGFP

Mutation

deleted amino acids 1-237,449-685

Promoter

CMV

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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