We narrowed to 2,256 results for: D10

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pCMV_UdgX-EE-UdgX-NG-nCas9

Plasmid

#163570

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-EE-UdgX-NG-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pMyc-CMV1-huTLR4mut-C2141A

Plasmid

#53527

Purpose

expression of mutant TLR4 in mammalian cells

Depositor

Linda Yu

Article

Kuo et al Cell Death Differ. 2015 Jan 30. doi: 10.

Insert

TLR4 C2141A (TLR4 Human)

Tags

Myc

Expression

Mammalian

Mutation

C2141A

Promoter

CMV

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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pMyc-CMV1-huTLR4mut-C1196T

Plasmid

#53526

Purpose

expression of mutant TLR4 in mammalian cells

Depositor

Linda Yu

Article

Kuo et al Cell Death Differ. 2015 Jan 30. doi: 10.

Insert

TLR4 C1196T (TLR4 Human)

Tags

Myc

Expression

Mammalian

Mutation

C1196T

Promoter

CMV

Available Since

July 21, 2014

Availability

Academic Institutions and Nonprofits only

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pMyc-CMV1-huTLR4mut-A896G

Plasmid

#53525

Purpose

expression of mutant TLR4 in mammalian cells

Depositor

Linda Yu

Article

Kuo et al Cell Death Differ. 2015 Jan 30. doi: 10.

Insert

TLR4 A896G (TLR4 Human)

Tags

Myc

Expression

Mammalian

Mutation

A896G

Promoter

CMV

Available Since

July 21, 2014

Availability

Academic Institutions and Nonprofits only

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L4207 TNFR2 NTEVp chain in PolyTX-mNeonGreen

Plasmid

#244175

Purpose

Constitutive expression of a MESA NTEVp chain encoding (N to C): TNFR2SS-3xFLAG-TNFR2ECD-mCD28TMD-NTEVp(75S)

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

MESA NTEVp chain with human TNFR2 SS and ECD, murine CD28 TMD, and NTEVp (75S) (TNFRSF1B Synthetic, Human)

Use

Synthetic Biology

Tags

TNFR2 signal peptide - 3xFLAG

Expression

Mammalian

Mutation

NTEVp_75S

Promoter

hEF1a

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pKMW281_dSpRY

Plasmid

#244822

Purpose

Mammalian expression of catalytically inactive SpRY Cas9 (dSpRY)

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

dSpRY Cas9

Use

CRISPR

Tags

3xFLAG-SV40 NLS and Nucleoplasmin NLS

Expression

Mammalian

Mutation

D10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…

Promoter

CAG

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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STIM1ct-ssrA2-mCerulean

Plasmid

#223690

Purpose

PhoBIT2 component; ssrA2 tag (ssrA mutant A2C) inserted into mCerulean-tagged STIM1 cytoplasmic domain

Depositor

Yubin Zhou

Article

Lee et al Nat Commun. 2025 Jul 28;16(1):6940. doi:

Insert

STIM1(233-685)-ssrA2 (STIM1 Human)

Tags

mCerulean

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-sp-myc-hSema4D-Q497P

Plasmid

#190655

Purpose

Expresses Human Sema4D with N terminal myc after signal peptide containing 497P mutation

Depositor

Andrew Newman, Victor Tarabykin

Article

Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

Insert

Sema4D (SEMA4D Human)

Tags

myc

Expression

Mammalian

Mutation

497P

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-sp-flag-hSema4D-Q497P

Plasmid

#190657

Purpose

Expresses Human Sema4D with N terminal flag after signal peptide containing 497P mutation

Depositor

Andrew Newman, Victor Tarabykin

Article

Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

Insert

Sema4D (SEMA4D Human)

Tags

flag

Expression

Mammalian

Mutation

497P

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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pUHD 10-3-human CDK11 p110B D543→N mut. cat.-FLAG

Plasmid

#240139

Purpose

Express CDK11 p110B D543N mutant

Depositor

Vincent Kidd, Jill Lahti, Martine Roussel

Article

Loyer et al J Biol Chem. 2008 Mar 21;283(12):7721-32

Insert

CDK11 p110 (CDK11A Human)

Tags

FLAG

Expression

Mammalian

Mutation

D543N

Promoter

TRE, CMV

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-ExRai-CKAR2

Plasmid

#236100

Purpose

Enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells, targeted to lysosome surface.

Depositor

Jin Zhang

Article

Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

Insert

LAMP1-ExRai-CKAR2 (LAMP1 Synthetic, Human)

Tags

Full-length LAMP1

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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R2pH-LAMP-FRB

Plasmid

#225138

Purpose

R2pH-LAMP1-labelled lysosomes with FRB

Depositor

Massimiliano Stagi

Article

Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

Insert

Lamp1 (Lamp1 Mouse)

Expression

Mammalian

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pmcherry-mTet1s pc3905

Plasmid

#201703

Purpose

Expresses of mcherry-tagged mTet1s in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

Tet1s (Tet1 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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TH1534-DuET

Plasmid

#213246

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

TH1534 (FZD10 Human)

Tags

1D4 and H4

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pNM1325

Plasmid

#197144

Purpose

CAGp-Target-AID-2A-BlastR for PiggyBac genomic integration

Depositor

Nozomu Yachie

Article

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

SpCas9-PmCDA1-UGI

Use

CRISPR

Expression

Mammalian

Mutation

SpCas9(D10A)

Promoter

CAG

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pFlare9A-CD45i4e5i5mutCBS

Plasmid

#131835

Purpose

A reporter construct containing the Exon 5 of PTPRC (CD45), which contains a mutant CTCF binding site, making the exon to be always excluded.

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

PTPRC (PTPRC Human)

Expression

Mammalian

Mutation

partial intron 4 full exon 5 and partial intron 5…

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_RBMX-eA3A-UdgX-HF-NG-nCas9

Plasmid

#163550

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

RBMX-eA3A-UdgX-HF-NG-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)N497A, R661A, Q695A, Q926A//L111R, D1…

Available Since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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T7pr-His6-MBP-TEV-Cas9-BirA*

Plasmid

#159993

Purpose

Bacterial expression of Cas9-BirA*

Depositor

Jacob Corn

Article

Wang et al Mol Cell. 2020 Jul 16;79(2):221-233.e5.

Insert

Cas9-BirA*

Tags

6X-His, MBP, 3X-FLAG, NLS

Expression

Bacterial

Mutation

D10A, H840A

Promoter

T7

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pJT175_GalL_A3A(Y130F)Δ186-BE3

Plasmid

#145124

Purpose

Expressing base editor A3A(Y130F)Δ186-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3A(Y130F)Δ186-BE3

Use

CRISPR

Expression

Yeast

Mutation

A3A(Y130F; 1-186AA); spCas9(D10A)

Promoter

GalL

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-EFS-SaABE8e-bGH-U6-sgRNA-BsmBI

Plasmid

#189922

Purpose

AAV genome encoding SaABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

Depositor

David Liu

Article

Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

Insert

SaABE8e

Use

AAV

Mutation

SaCas9 D10A

Promoter

EFS

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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