We narrowed to 3,117 results for: multiplex
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Plasmid#226168PurposeLentiviral plasmid used to express a TurboID fusion of the protein kinase CRaf.DepositorInsertMyc-TurboID-CRaf (RAF1 Human)
UseLentiviralTagsEGFP-IRES-BlastR and Myc-TurboIDExpressionMammalianAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
MTNR1B-DuET
Plasmid#213349PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
MTNR1A-DuET
Plasmid#213348PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_GR-RE_MLPmin_BC1094-luc2
Plasmid#227114PurposeGlucocorticoid receptor response element for luciferase and barcode assays; barcode BC1094DepositorInsert12x clustered GR response element linked to MLP minimal promoter driving barcode 1094 and a luciferase reporter gene
UseAAVExpressionMammalianAvailable SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBA409
Plasmid#85971PurposeEF1a-sfGFP-Ubc-NeoDepositorInsertssfGFP
Neo
UseLentiviralExpressionMammalianAvailable SinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(4,5)P2 Probe
Plasmid#211513PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-4,5-Bisphosphate (PI(4,5)P2) from bacteriaDepositorInsertPLCdelta1-PH
Tags6xhis-SNAPExpressionBacterialAvailable SinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-dInPAkt
Plasmid#181834PurposeGenetically encoded single-color sensor for monitoring plasma membrane PI(3,4,5)P2 dynamics in living cells. Based on dimerization-dependent red fluorescent protein (ddRFP).DepositorInsertLyn-dInPAkt
TagsN-terminal targeting sequence from Lyn kinase, dd…ExpressionMammalianPromoterCMVAvailable SinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
TRE reporter backbone
Plasmid#158678PurposeThis plasmid serves as the backbone for the TRE reporters described in our paper. It's a promoterless-mStrawberry-pGK-BSD backbone to which we insert a pathway specific promoter before the mStrawberryDepositorTypeEmpty backboneUseLentiviralTagsmStrawberryExpressionMammalianAvailable SinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(3,4,5)P3 Probe
Plasmid#211514PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3,4,5-Trisphosphate (PI(3,4,5)P3) from bacteriaDepositorInsertBTK1-PH
Tags6xhis-SNAPExpressionBacterialAvailable SinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(3,4)P2 Probe
Plasmid#211511PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3,4-Bisphosphate (PI(3,4)P2) from bacteriaDepositorInsertTAPP1 cPHx3
Tags6xhis-SNAPExpressionBacterialAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
iRFP-H2A
Plasmid#158691PurposeThis plasmid encodes an iRFP fused to H2A histone nuclear marker. It has CMV promoter driving the expression of iRFP fused to H2A. It has no selection markersDepositorInsertiRFP670
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL1777 (pEffector)
Plasmid#160731PurposeEffector expression for V. cholerae CAST system, encodes all protein and RNA components. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pCDF backbone.DepositorInsertVchCAST proteins and crRNA
ExpressionBacterialPromoterJ23119Available SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ173
Plasmid#99907PurposeExpress CRISPR-Act2.0 system which contains pco-dCas9-VP64 fusion protein and MS2-VP64 fusion protein linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequenceDepositorInsertpco-dCas9-VP64-T2A-MS2-VP64
UseCRISPRExpressionPlantAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Akt-FoxO3a-KTR-EGFP
Plasmid#125131PurposeFluorescent reporter for Akt activityDepositorInsertFOXO2 (FOXO3 Human)
TagsEnhanced green fluorescent protein (EGFP)ExpressionMammalianMutationFoxO3A 1-402 amino acidPromoterCAG promoterAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRDA_816
Plasmid#201157PurposeEnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' VP64 and NLS for CRISPRaDepositorInsert5x-ALFA tag-dEnAsCas12a-VP64
UseCRISPR and LentiviralMutationD908APromoterEFSAvailable SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHUE411
Plasmid#62203PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Hyg resistanceDepositorInsertsgRNA scaffold
zCas9
UsePlant binary vectorTags3x FLAG and NLSExpressionPlantMutationmaize codon optimizedPromoterOsU3 and UbiAvailable SinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC0068 PspCas13 (B12) His6-TwinStrep-SUMO-BsaI
Plasmid#115219PurposeExpresses PspCas13b from a SUMO-tagged bacterial expression constructDepositorInsertPspCas13
TagsSUMOExpressionBacterialAvailable SinceSept. 21, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHAGE-EFS-MCP-3XBFP-2xNLS
Plasmid#236371PurposeOverexpression of MCP-3XBFP-2xNLS in human cellsDepositorInsertMCP-3XBFP-2xnls
UseLentiviralAvailable SinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
PGK1p-Csy4-pA (Construct 2)
Plasmid#55196PurposeExpresses Csy4 in Mammalian cells for site-specific RNA processingDepositorInsertCsy4/Cas6
UseSynthetic BiologyTags6xHISExpressionMammalianPromoterCMVAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDGE347
Plasmid#153228PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only