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pUPD3_PARTPRM_Pem7

Plasmid

#243919

Purpose

GoldenBraid2.0 Strong, constitutive promoter, not including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

Pem7

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pBSKa1_Stuffer1

Plasmid

#243844

Purpose

GoldenBraid2.0 Full transcription unit stuffer in an alpha-level cloning vector.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

Stuffer1

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pBSKa2_Stuffer2

Plasmid

#243847

Purpose

GoldenBraid2.0 Full transcription unit stuffer in an alpha-level cloning vector.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

Stuffer2

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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POC1343

Plasmid

#221568

Purpose

Plasmid containing the Insert 1 to be assembled in POC1355

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by BsaI sites

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1344

Plasmid

#221569

Purpose

Plasmid containing the Insert 2 to be assembled in POC1355

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by BsaI sites

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1345

Plasmid

#221570

Purpose

Plasmid containing the Insert 3 to be assembled in POC1355

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by BsaI sites

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1346

Plasmid

#221571

Purpose

Plasmid containing the Insert 4 to be assembled in POC1355

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by BsaI sites

Use

Synthetic Biology

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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psLIB U6

Plasmid

#229956

Purpose

bigMamAct is evolution of biGBac for mammalian cells. Has empty U6-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pX458 ACTB

Plasmid

#229975

Purpose

ecnodes for spCas9 and sgRNA targeting last intron of ActinB gene

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Insert

ActBsgRNA and spCas9

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti puro HA-STX3

Plasmid

#227590

Purpose

Lentiviral plasmid expressing human STX3 protein

Depositor

Melina Fan, Addgene Research Program

Insert

STX3 (STX3 Human)

Use

Lentiviral

Tags

HA

Promoter

CMV

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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