Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 1,094 results for: gibson assembly

Clear keywords
Showing: 951 - 960 of 1094 results

  1. pBD21_034

    Plasmid
    #109151
    Purpose
    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of TagBFP
    Depositor
    Ron Weiss
    Article
    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1
    Inserts
    mRFP
    TagBFP
    Use
    Synthetic Biology
    Expression
    Bacterial and Mammalian
    Promoter
    CMV and pTet
    Available Since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    109151-plasmid-map-sequence-id-220104

    Enlarge

  2. pZH512

    Plasmid
    #102666
    Purpose
    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    102666-plasmid-map-sequence-id-199387

    Enlarge

  3. pZH511

    Plasmid
    #102665
    Purpose
    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    102665-plasmid-map-sequence-id-199381

    Enlarge

  5. pHRSV_CHARMS-2xTIR

    Plasmid
    #233930
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-2xTIR which contain a tandem dimer of TIR domains
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-2xTIR
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    Aug. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233930-plasmid-map-sequence-id-484962

    Enlarge

  7. pHRSV_CHARMS-bDD-5xT6BMs

    Plasmid
    #233939
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-bDD with 5 TRAF6 binding motifs
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-bDD-5xT6BMs
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233939-plasmid-map-sequence-id-469210

    Enlarge

  8. pHRSV_CHARMS-V43D/E52K/R62E

    Plasmid
    #233940
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS three mutations in the death domain that disrupt oligomer formation
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-V43D/E52K/R62E
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233940-plasmid-map-sequence-id-469200

    Enlarge

  9. pHRSV_CHARMS-1x

    Plasmid
    #233923
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-1x which contains only 1 TRAF6 binding motif
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-1x
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233923-plasmid-map-sequence-id-469219

    Enlarge

  10. pHRSV_CHARMS-1xA

    Plasmid
    #233924
    Purpose
    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-1xA which contains 1 TRAF6 binding motif with a alanine mutation
    Depositor
    Marcus Taylor
    Article
    Lichtenstein et al bioRxiv 2024.05.08.593169
    Insert
    CHARMS-1xA
    Use
    Lentiviral
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    SV40
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    233924-plasmid-map-sequence-id-468361

    Enlarge

Showing: 951 - 960 of 1094 results