We narrowed to 4,427 results for: Alk

1. pBADHISTEV_PfeEH258A

   Plasmid

   #129122

   Purpose

   expresses H258A mutant of PfeE in E.coli

   Depositor

   James Naismith

   Article

   Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

   Insert

   Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
   

   Use

   Tags

   TEV protease cleavable 6xHis

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed, mutat…

   Promoter

   PBAD

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBADHISTEV_PfeES157A

   Plasmid

   #129121

   Purpose

   expresses S157A mutant of PfeE in E.coli

   Depositor

   James Naismith

   Article

   Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

   Insert

   Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
   

   Use

   Tags

   TEV protease cleavable 6xHis

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed, mutat…

   Promoter

   PBAD

   Available since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HA tagged Sph ELL2

   Plasmid

   #127270

   Purpose

   For in vitro translation of human WT ELL2 with internal HA tag

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (ELL2 Human)
   

   Use

   In vitro translation

   Tags

   Expression

   Mutation

   WT Mets and HA tag at SphI site (8th Met)

   Promoter

   T7

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA tagged mid ELL2

   Plasmid

   #127274

   Purpose

   Expresses human ELL2 with HA tag in the middle that does not disrupt function

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 with HA at XbaI (ELL2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HA inserted at XbaI after Met 186

   Promoter

   pEF1a

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Met138ILeu ELL2

   Plasmid

   #127260

   Purpose

   For in vitro translation of human ELL2 with Met138 mutated to Ileu to ask if there is internal initiation

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (Met138 to Ileu) (ELL2 Human)
   

   Use

   In vitro translation

   Tags

   Expression

   Mutation

   Met138 to Ileu Quikchange

   Promoter

   T7

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Met186ILeu ELL2

   Plasmid

   #127261

   Purpose

   For in vitro translation of human ELL2 with Met186 mutated to Ileu to ask if there is internal initiation

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (Met186 to Ileu) (ELL2 Human)
   

   Use

   In vitro translation

   Tags

   Expression

   Mutation

   Met186 to Ileu Quikchange

   Promoter

   T7

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Met138ILeu, Met186ILeu ELL2

   Plasmid

   #127265

   Purpose

   For in vitro translation of human ELL2 with Met138ILeu, Met186ILeu

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (Met138ILeu, Met186ILeu) (ELL2 Human)
   

   Use

   In vitro translation

   Tags

   Expression

   Mutation

   Two Mets (138,186) to ILeu Quikchange

   Promoter

   T7

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Met133ILeu ELL2

   Plasmid

   #127259

   Purpose

   For in vitro translation of human ELL2 with Met133 mutated to Ileu to ask if there is internal initiation

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (Met133 to Ileu) (ELL2 Human)
   

   Use

   In vitro translation

   Tags

   Expression

   Mutation

   Met133 to Ileu Quikchange

   Promoter

   T7

   Available since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Met133ILeu, Met186ILeu ELL2

   Plasmid

   #127264

   Purpose

   For in vitro translation of human ELL2 with Met133ILeu, Met186ILeu

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   ELL2 (Met133ILeu, Met186ILeu) (ELL2 Human)
   

   Use

   In vitro translation

   Tags

   Expression

   Mutation

   Two Mets (133,186 )to ILeu Quikchange

   Promoter

   T7

   Available since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Met331Leu ELL2

    Plasmid

    #127262

    Purpose

    For in vitro translation of human ELL2 with Met331 mutated to Ileu to ask if there is internal initiation

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met331 to Ileu) (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    Met331 to ILeu Quikchange

    Promoter

    T7

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. COOHend ELL2

    Plasmid

    #127255

    Purpose

    Expresses the C terminal segment of human ELL2 in mammalian cells

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (C-terminal end) (ELL2 Human)
    

    Use

    Other

    Tags

    Expression

    Mammalian

    Mutation

    contains only the last 389 aa

    Promoter

    pEF1a

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. Met1ILeu ELL2

    Plasmid

    #127258

    Purpose

    For in vitro translation of human ELL2 with Met1 mutated to Ileu to ask if there is internal initiation

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (Met1 to Ileu) (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    Met1 to Ileu by Quikchange

    Promoter

    T7

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ELL2mRNA+polyA

    Plasmid

    #127256

    Purpose

    For in vitro translation of human ELL2 with 30 nt pA tail

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    shortens 3'UTR to 40 bp adds A30 tail

    Promoter

    T7

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. NH2end ELL2

    Plasmid

    #127252

    Purpose

    Expresses the N terminal segment of human ELL2 in mammalian cells

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (1-287) (ELL2 Human)
    

    Use

    Other

    Tags

    His6X

    Expression

    Mammalian

    Mutation

    contains only amino acids 1-289

    Promoter

    pEF1a

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HA tagged Xba ELL2

    Plasmid

    #127268

    Purpose

    For in vitro translation of WT ELL2 with HA tag inserted near N-term

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    ELL2 (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    ELL2mRNA+polyA with HA tag inserted at aa12 betwe…

    Promoter

    T7

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

16. TRE-Lepr_FL-IRES-eGFP

    Plasmid

    #196635

    Purpose

    Doxycycline inducible expression of full length mouse Lepr with eGFP marker

    Depositor

    Elaine Fuchs

    Article

    Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

    Insert

    Lepr (Lepr Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. 8xNFAT-ZsG-hCD8

    Plasmid

    #153417

    Purpose

    NFAT-driven ZsGreen-1 reporter gene with human CD8ab gene

    Depositor

    Maki Nakayama

    Article

    Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

    Insert

    8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (Cd8a )
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLVX-TetOne-Puro-hAXL

    Plasmid

    #124797

    Purpose

    Tet-inducible AXL expression in mammalian cells

    Depositor

    Kenneth Pienta

    Article

    Axelrod et al Mol Cancer Res. 2019 Feb;17(2):356-369.

    Insert

    AXL (AXL Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GS

    Available since

    June 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLentiCMVNeoDEST(705-1)-hAXL

    Plasmid

    #124796

    Purpose

    Expresses AXL in mammalian cells

    Depositor

    Kenneth Pienta

    Article

    Axelrod et al Mol Cancer Res. 2019 Feb;17(2):356-369.

    Insert

    AXL (AXL Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBABEpuro-gateway

    Plasmid

    #51070

    Purpose

    gateway adapted mammalian expression vector

    Depositor

    Matthew Meyerson

    Article

    Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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