Addgene

We narrowed to 5,096 results for: PEP

Showing: 1901 - 1920 of 5096 results

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pMXs-PT2A-iRFP670-GFP-Tdtomato

Plasmid

#111816

Purpose

Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Inserts

iRFP670
GFP
Tdtomato

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-BirA-eCPX-AP

Plasmid

#121907

Purpose

Expresses BirA and eCPX-AP

Depositor

Per Svenningsen

Article

Granhoj et al Sci Rep. 2019 Mar 11;9(1):4118. doi: 10.

Inserts

BirA (birA Synthetic)
Enhanced Circularly Permuted Outer Membrane Protein X

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7 and pBAD promoter

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pFP.R275

Plasmid

#138224

Purpose

Expresses the split mCherry reporter interrupted by the PhoRadA intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(SGK).PhoRadA.(TQL)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

P(araBAD) and iPC

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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plx208-CMV-Gal4DBD-CapN-SsrA-CapC

Plasmid

#213533

Purpose

Expresses one of the split transcription factor component in mammalian cells

Depositor

Wenjing Wang

Article

Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

Insert

SspB-EGFP-VP16

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5-Flag-HA-Merlin

Plasmid

#27104

Depositor

Filippo Giancotti

Article

Li et al Cell. 2010 Feb 19. 140(4):477-90.

Insert

Merlin (Nf2 Mouse)

Use

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
Bov.hsc70.RSET.FL.wt(NarI)

Plasmid

#12532

Depositor

David McKay

Article

Ha et al J Biol Chem. 1997 Oct 31. 272(44):27796-

Insert

bovine 70 kDa heat shock cognate protein full length wild type

Use

Tags

Expression

Bacterial

Mutation

wild type

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

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pFP.R371

Plasmid

#138241

Purpose

Expresses the split mCherry reporter interrupted by the MP-M-DnaB artificially mini-intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(NGK).MP-M-DnaB*.(TEL)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

P(araBAD) and iPC

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pGEMT-PT2A-Tdtomato-iRFP670-GFP

Plasmid

#111815

Purpose

Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Inserts

Tdtomato
iRFP670
GFP

Use

Cloning intermediate

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pETa(+) HpCsd3 (42-403)

Plasmid

#71285

Purpose

Expresses Csd3 from Helicobacter pylori

Depositor

SeWon Suh

Article

An et al Acta Crystallogr D Biol Crystallogr. 201

Insert

HP0506 (HP0506 )

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
pTubulin-BC2T

Plasmid

#108970

Purpose

expresses tubulin-BC2-tag in mammalian cells

Depositor

Ulrich Rothbauer

Article

Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

Insert

a-tubulin

Use

Tags

BC2-tag

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCX-2A-2A

Plasmid

#74670

Purpose

based on pCX-C1 with F2A1/F2A2 inserted

Depositor

Roberto Giovannoni

Article

De Giorgi et al Plasmid. 2015 May;79:22-9. doi: 10.1016/

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCAGGS

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pT7-agrBD-I

Plasmid

#53439

Purpose

T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrBD locus

Use

Synthetic Biology

Tags

V5 epitope, 6x HIS (not expressed on agrB or agrD…

Expression

Bacterial

Mutation

none

Promoter

T7-lac

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pZA-GFP-R5

Plasmid

#106256

Purpose

Constitutively expresses GFP fused with silaffin-R5 silica affinity peptide

Depositor

Urartu Seker

Article

Olmez et al ACS Omega. 2018 Jan 31;3(1):585-594. doi

Insert

Green fluorescent protein

Use

Tags

Added silaffin R5 peptide to C terminus of GFP af…

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pILGFPU0 (R)

Plasmid

#83562

Purpose

Express yEGFP-ERG9 ER targeting peptide-CLN2PEST under the control of TEF1 promoter

Depositor

Claudia Vickers

Article

Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

Insert

TEF1 promoter-yEGFP-ERG9 ER targeting peptide-CLN2PEST

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pFP.R400

Plasmid

#138175

Purpose

Expresses the split mCherry reporter interrupted by the chimeric Npu/SspDnaE intein (in cis)

Depositor

Baojun Wang

Article

Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

Inserts

araC (araC )
mCherryN(GGK).NpuSsp*.(CWN)mCherryC.H6

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

P(araBAD) and iPC

Available since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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pWKS1583

Plasmid

#70188

Purpose

Modular vector for secreted amylase reporter expression

Depositor

Wiep Klaas Smits

Article

Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

Insert

amyEopt

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Ptet

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-Merlin-Flag-HA

Plasmid

#27103

Depositor

Filippo Giancotti

Article

Li et al Cell. 2010 Feb 19. 140(4):477-90.

Insert

Merlin (Nf2 Mouse)

Use

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1: TfR1-CoilE

Plasmid

#181988

Purpose

CoilE genetically encoded tag on Transferrin Receptor 1 (TfR1) to be used with CoilR labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

TfR1-CoilE

Use

Tags

CoilE

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_RAC1_Ras

Plasmid

#110030

Purpose

Protein expression and purification of RAC1_Ras

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RAC1_Ras (RAC1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_UEVLD_UEV

Plasmid

#109902

Purpose

Protein expression and purification of UEVLD_UEV

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

UEVLD_UEV (UEVLD Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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