We narrowed to 9,801 results for: pET

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pET-6xHis-GFP(-6)-H2_mCherry(-6)-L1

Plasmid

#205050

Purpose

Co-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

GFP(-6)-H2_mCherry(-6)-L1

Tags

GRRGKKSRK and MGHHHHHGGA

Expression

Bacterial

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(-6)-H2_mCherry(-6)-L2

Plasmid

#205051

Purpose

Co-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

GFP(-6)-H2_mCherry(-6)-L2

Tags

GRRGKKSRKGRRGKKSRK and MGHHHHHGGA

Expression

Bacterial

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(-6)-H2_mCherry(-6)-L3

Plasmid

#205052

Purpose

Co-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

GFP(-6)-H2_mCherry(-6)-L3

Tags

GRRGKKSRKGRRGKKSRKGRRGKKSRK and MGHHHHHGGA

Expression

Bacterial

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET-tTALE-mTurquoise2-15b

Plasmid

#206871

Purpose

Express tTALE-mTurquoise2

Depositor

Kyubong Jo

Article

Kim et al Molecules. 2022 Aug 17;27(16):5248. doi:

Insert

7-mer tTALE-mTurquoise2

Expression

Bacterial

Promoter

T7 promoter

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pET-tTALE-mNeonGreen-15b

Plasmid

#206870

Purpose

Express tTALE-mNeonGreen

Depositor

Kyubong Jo

Article

Kim et al Molecules. 2022 Aug 17;27(16):5248. doi:

Insert

7-mer tTALE-mNeonGreen

Expression

Bacterial

Promoter

T7 promoter

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pET-tTALE-mKO2-15b

Plasmid

#206869

Purpose

Express tTALE-mKO2

Depositor

Kyubong Jo

Article

Kim et al Molecules. 2022 Aug 17;27(16):5248. doi:

Insert

7-mer tTALE-mKO2

Expression

Bacterial

Promoter

T7 promoter

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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PET-HypaSpCas9-NLS-6xHis

Plasmid

#207384

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

HypaSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N692A, M694A, Q695A, H698A

Promoter

T7

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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PET-HeFSpCas9-NLS-6xHis

Plasmid

#207391

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

HeFSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A

Promoter

T7

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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PET-evoSpCas9-NLS-6xHis

Plasmid

#207389

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

evoSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

M495V, Y515N, K526E, R661Q

Promoter

T7

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pET-28b(+)/ADK C77S

Plasmid

#201409

Purpose

Overexpresses Escherichia coli adenylate kinase C77S

Depositor

Gary Pielak

Article

Brom et al Biomol NMR Assign. 2023 Aug 26. doi: 10.

Insert

Escherichia coli adenylate kinase C77S (adk Escherichia coli)

Expression

Bacterial

Mutation

changed cysteine 77 to serine

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoB-CaM

Plasmid

#193812

Purpose

Protein production of ChemoB-CaM in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoB-CaM

Tags

10xHis

Expression

Bacterial

Mutation

EBFP2: N39Y, V206K

Promoter

T7

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoC-CaM

Plasmid

#193813

Purpose

Protein production of ChemoC-CaM in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoC-CaM

Tags

10xHis

Expression

Bacterial

Promoter

T7

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoY-CaM

Plasmid

#193814

Purpose

Protein production of ChemoY-CaM in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoY-CaM

Tags

10xHis

Expression

Bacterial

Mutation

Venus:A206K

Promoter

T7

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoR-CaM

Plasmid

#193815

Purpose

Protein production of ChemoR-CaM in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoR-CaM

Tags

10xHis

Expression

Bacterial

Mutation

mScarlet: D201K

Promoter

T7

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET PI-SceI C-His K403A

Plasmid

#195612

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K403A active site mutation

Depositor

Frederick Gimble

Article

Gimble et al J Biol Chem. 1998 Nov 13;273(46):30524-9

Insert

PI-SceI

Expression

Bacterial

Mutation

A1463G, A1464C

Promoter

T7

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET PI-SceI C-His K301A

Plasmid

#195611

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K301A active site mutation

Depositor

Frederick Gimble

Article

He et al J Biol Chem. 1998 Feb 20;273(8):4607-15.

Insert

PI-SceI

Expression

Bacterial

Mutation

A822G, A823C, A824T

Promoter

T7

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-His(6)-AtPCO1(wt)

Plasmid

#191798

Purpose

Full length wt AtPCO1 with Nt-His tag

Depositor

Emily Flashman

Article

White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

Insert

AtPCO1

Tags

6xHis, Thrombin cleavage site

Expression

Bacterial

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-His(6)-AtPCO2(wt)

Plasmid

#191799

Purpose

Full length wt AtPCO2 with Nt-His tag

Depositor

Emily Flashman

Article

White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

Insert

AtPCO2

Tags

6xHis, Thrombin cleavage site

Expression

Bacterial

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-His(6)-AtPCO3(wt)

Plasmid

#191800

Purpose

Full length wt AtPCO3 with Nt-His tag

Depositor

Emily Flashman

Article

White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

Insert

AtPCO3

Tags

6xHis, Thrombin cleavage site

Expression

Bacterial

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP2-E67A_rpoA-U

Plasmid

#190959

Purpose

Test the U-to-C editing activity of KP2:E67A on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP2

Tags

His

Expression

Bacterial

Mutation

DYW-KP:E67A

Promoter

T7

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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