We narrowed to 10,632 results for: t7

1. TC9nickpAB

   Plasmid

   #71311

   Purpose

   used as a template for in vitro transcription of Cas9 nickase mRNA after linearizing with BbsI

   Depositor

   Karl Degenhardt

   Insert

   Cas9 nickase
   

   Use

   In vitro transcription

   Tags

   NLS

   Mutation

   D10A nickase

   Promoter

   T7

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. 6P0Q

   Plasmid

   #162262

   Purpose

   Baculovirus expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   WDR12 (WDR12 Human)
   

   Tags

   TEV-6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 17, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pJK690

   Plasmid

   #72380

   Purpose

   Produces Escherichia coli monothiol glutaredoxin GrxD, N-terminal His6 tag (H6EcGrxD)

   Depositor

   T. J. Kappock

   Insert

   glutaredoxin-4
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJK551

   Plasmid

   #72425

   Purpose

   Produces Acetobacter aceti 1023 putative acyl-CoA:carboxylate CoA-transferase, N-terminal His6 tag (H6AaUctD)

   Depositor

   T. J. Kappock

   Insert

   putative CoA-transferase
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmCellFree_KA1_SARS-COV-2_NSP15

   Plasmid

   #169388

   Purpose

   Cell-free/mammalian expression vector for SARS-COV-2 virus NSP15 with N-term eGFP

   Depositor

   Kirill Alexandrov

   Insert

   SARS-COV-2 _NSP15
   

   Use

   Cell-free expression vector

   Tags

   His and eGFP

   Promoter

   T7- CMV/CAG

   Available Since

   July 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmCellFree_KA1_SARS-COV-2_NSP5

   Plasmid

   #169380

   Purpose

   Cell-free/mammalian expression vector for SARS-COV-2 virus NSP5 with N-term eGFP

   Depositor

   Kirill Alexandrov

   Insert

   SARS-COV-2 _NSP5
   

   Use

   Cell-free expression vector

   Tags

   His and eGFP

   Promoter

   T7- CMV/CAG

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK402

   Plasmid

   #72415

   Purpose

   Produces Acetobacter aceti 1023 Est03, N-terminal His6 tag

   Depositor

   T. J. Kappock

   Insert

   4-hydroxybenzoyl-CoA thioesterase
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. SHC1

   Plasmid

   #73255

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   SHC1 (SHC1 Human)
   

   Tags

   His-6-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. 5C8G

   Plasmid

   #69968

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   Tb427tmp.01.1830:MAC054-B05:C234722
   

   Tags

   His and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7-lacO

   Available Since

   Oct. 7, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

10. 4MX2

    Plasmid

    #60102

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    LdBPK_040440.1:MAC04A-B07:C226244
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

11. SPIN3A

    Plasmid

    #74656

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    SPIN3A (SPIN3 Human)
    

    Tags

    TEV-His6-Flag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pmCellFree_KA1_SARS-COV-2_NSP4

    Plasmid

    #169379

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP4 with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    SARS-COV-2 _NSP4
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Promoter

    T7- CMV/CAG

    Available Since

    Aug. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmCellFree_KA1_SARS-COV-2_NSP8

    Plasmid

    #169382

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP8 with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    SARS-COV-2 _NSP8
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Promoter

    T7- CMV/CAG

    Available Since

    July 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmCellFree_KA1_SARS-COV-2_NSP13

    Plasmid

    #169386

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP13 with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    SARS-COV-2 _NSP13
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Promoter

    T7- CMV/CAG

    Available Since

    July 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJK532

    Plasmid

    #72513

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit with N-terminal His6 tag (H6AaPurQ)

    Depositor

    T. J. Kappock

    Insert

    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. 4QN1

    Plasmid

    #61944

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SHPRH (SHPRH Human)
    

    Tags

    MHHHHHHSSGRENLYFQG

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    March 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

17. human EB1 in pBluescript II

    Plasmid

    #39324

    Depositor

    Tim Mitchison, Jennifer Tirnauer

    Insert

    EB1 (MAPRE1 Human)
    

    Expression

    Bacterial and Mammalian

    Promoter

    T7

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

18. PARP9

    Plasmid

    #73256

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PARP9 (PARP9 Human)
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR221_UBE2V1_3UTR

    Plasmid

    #65864

    Purpose

    Carries 3' UTR of UBE2V1, an RBPMS target

    Depositor

    Thomas Tuschl

    Insert

    UBE2V1 (UBE2V1 Human)
    

    Promoter

    T7

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pJK544

    Plasmid

    #72517

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS)

    Depositor

    T. J. Kappock

    Insert

    formylglycinamidine ribonucleotide synthetase, small subunit
    

    Expression

    Bacterial

    Mutation

    contains additional Ala2

    Promoter

    T7

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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