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Plasmid#85736PurposeExpresses a truncated His-tagged GvpC from Anabaena flos-aquae without the N-and C-terminal regions in E.coliDepositorInsertGas vesicle protein C (truncated and his-tagged)
UseTagsHis-tagExpressionBacterialMutationDeletion of N and C terminal regions, addition of…PromoterT7Available sinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-HIS-CBM-CohI
Plasmid#58709PurposeProbing molecule to perform single molecule force spectroscopy experimentsDepositorInsertCBM-CohI
UseTagsHIS, HRV 3C, and ybbRExpressionBacterialMutationchanged Alanine 2 to Cysteine in CBM domainPromoterT7Available sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28b-NmCas9-Flag D16A+H588A
Plasmid#109033PurposeD16A+H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expressionDepositorInsertCRISPR-associated endonuclease Cas9
UseTagsFlag TagExpressionBacterialMutationPromoterT7Available sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 I136A, I139A, M142A
Plasmid#74617PurposeExpresses a triple mutant truncation of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-239, I136A, I139A, M142A (HOOK3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationD102A, E108APromoterT7Available sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)_S. cerevisiae Hbs1(166-611)
Plasmid#39338DepositorInsertElongation factor 1 alpha-like protein
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-HIS-10FNIII(x4)-DocI
Plasmid#58712PurposeCharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tagDepositorInsert10FNIII(x4)-DocI
UseTagsHIS, HRV 3C, and ybbRExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28b-SUMO-FLAG-TeKaiA-FLAG
Plasmid#229220PurposeFor expression of T. elongatus KaiA proteinDepositorInsertkaiA (kaiA T. elongatus)
UseTagsFLAG and SUMO-FLAGExpressionBacterialMutationPromoterAvailable sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b-SUMO-FLAG-TeKaiB-FLAG
Plasmid#229221PurposeFor expression of T. elongatus KaiB proteinDepositorInsertkaiB (kaiB T. elongatus)
UseTagsFLAG and SUMO-FLAGExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28b-SUMO-SeKaiB-K25C-FLAG
Plasmid#229225PurposeFor expression of S. elongatus KaiB protein mutantDepositorInsertkaiB (kaiB S. elongatus)
UseTagsFLAG and SUMOExpressionBacterialMutationK25CPromoterAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAM355 pET28a-GGG-Titin[V15P]-23-K-24-C-10
Plasmid#226351PurposeExpression of Titin (V15P) substrate for 26S proteasomeDepositorInserttitin (TTN Human)
UseTagsExpressionBacterialMutationV15PPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only