We narrowed to 4,386 results for: ARA-2

1. pTRE-BI-osTIR1

   Plasmid

   #207840

   Purpose

   Inducible TIR1 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

   Depositor

   Michaela Mueller-McNicoll

   Article

   Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

   Inserts

   osTIR
   mCherry
   mAID_-VHH-GFP4
   

   Tags

   3X HA-Tag and weak NLS

   Expression

   Mammalian

   Mutation

   All K mutated to R

   Promoter

   TRE3G BI promoter

   Available Since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-tetO-Ascl2

   Plasmid

   #71151

   Purpose

   Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Ascl2/Mash2

   Depositor

   Hubert Schorle

   Article

   Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

   Insert

   Ascl2 (Ascl2 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   tetO

   Available Since

   Nov. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP

   Plasmid

   #35509

   Purpose

   AAV expression of EF1a-driven, Cre-dependent, humanized channelrhodopsin E123T/T159C mutant fused to EYFP for optogenetic activation.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Has Service

   AAV5 and AAV9

   Insert

   hChR2
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E123T and T159C

   Promoter

   Ef1a

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLex 983-Micu2

   Plasmid

   #50372

   Purpose

   Expression in mammalian cells

   Depositor

   Vamsi Mootha

   Article

   Plovanich et al PLoS One. 2013;8(2):e55785. doi: 10.1371

   Insert

   MICU2 (MICU2 Human)
   

   Use

   Lentivirus

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pENT-L1-Kozak-GAP43-ECFP-stop-L2

   Plasmid

   #186356

   Purpose

   Entry clone with ORF encoding plasma membrane-targeted GAP43-eCFP flanked by Gateway recombination sequences

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Insert

   Growth-associated protein-43 (Gap43 Mouse, Synthetic)
   

   Use

   Expression of a fluorescent membrane marker

   Tags

   ECFP

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1_ 2xFLAG-2xSTREP_BACH1_Y11F

   Plasmid

   #159132

   Purpose

   Expresses BACH1 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

   Insert

   BACH1 (BACH1 Human)
   

   Tags

   2X-FLAG _ 2X-STREP

   Expression

   Mammalian

   Mutation

   Y11F

   Promoter

   CMV

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENT-L1-Kozak-Ensconsin-3XGFP-stop-L2

   Plasmid

   #186361

   Purpose

   Entry clone with ORF encoding the MT-binding domain of Ensconsin fused to 3 copies of GFP in C-terminal, flanked by Gateway recombination sequences.

   Depositor

   Patrick Lemaire

   Article

   Roure et al PLoS One. 2007. 2(9):e916.

   Insert

   Human Ensconsin (MAP7 Synthetic, Human)
   

   Use

   Expression of a fluorescent microtubule marker

   Tags

   3xEGFP

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPKm-230

   Plasmid

   #90499

   Purpose

   pSIN - EF-1alpha - PIF3 - MTAD - IRES - PhyB - GBD, dual vector of PIF3-MTAD under EF-1 alpha promoter and PhyB-DBD under IRES promoter; See growth conditions below.

   Depositor

   Todd Coleman

   Article

   Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

   Insert

   mito-tFD and mito-tFNR
   

   Use

   Lentiviral

   Available Since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. KA2880_pPB-TREtight-FLAG-Esrrb-InO

   Plasmid

   #124178

   Purpose

   piggyBac-based Dox-inducible expression of FLAG-Esrrb

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Insert

   FLAG-Esrrb-IRES-H2B-mKO2 (Esrrb Mouse)
   

   Expression

   Mammalian

   Promoter

   tetO-CMVmin

   Available Since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMTTH+mEGFP-LANA-H1.4CTD

    Plasmid

    #157802

    Purpose

    Expression of mEGFP, LANA peptide, and human linker histone H1.4 CTD fusion protein

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    mEGFP-LANA-H1.4CTD (H1-4 Human)
    

    Tags

    His6 and MBP

    Expression

    Bacterial

    Promoter

    pTAC

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. KA2944_pPB-TREtight-Esrrb-FLAG-InO

    Plasmid

    #124179

    Purpose

    piggyBac-based Dox-inducible expression of Esrrb-FLAG

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Esrrb-FLAG-IRES-H2B-mKO2 (Esrrb Mouse)
    

    Expression

    Mammalian

    Promoter

    tetO-CMVmin

    Available Since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPB9P3

    Plasmid

    #210034

    Purpose

    Contains cloning site for PE3/3b prime editing gRNA constructs, inducible prime editor 2, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    prime editor 2
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    EF-1α and TRE3G

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG_SRGAP2A_SH3(W765A)_GFP

    Plasmid

    #222616

    Purpose

    Expresses a form of full length SRGAP2 with one point mutation (W765A) in the SH3 domain abrogating its ability to bind to Gephyrin.

    Depositor

    Franck Polleux

    Article

    Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

    Insert

    SRGAP2 (SRGAP2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Point mutation W765A (SH3 domain)

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG_SRGAP2A_SH3(W765A)_HA

    Plasmid

    #222614

    Purpose

    Expresses a form of full length SRGAP2 with one point mutation (W765A) in the SH3 domain abrogating its ability to bind to Gephyrin.

    Depositor

    Franck Polleux

    Article

    Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

    Insert

    SRGAP2 (SRGAP2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Point mutation W765A (SH3 domain)

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG SRGAP2A_EVH1(P339L F342C)

    Plasmid

    #222613

    Purpose

    Expresses a form of full length SRGAP2 with two point mutations (P339L and F342C) that abrogate EVH1 binding site for Homer1

    Depositor

    Franck Polleux

    Article

    Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

    Insert

    SRGAP2 (SRGAP2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Point mutations P339L and F342C

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1α-Flex/3'USS-ChRWR/Venus(ATG mut)

    Plasmid

    #197891

    Purpose

    Can be used to generate AAV virus that will express ChRWR/Venus in the presence of Cre and the leakage expression is significantly reduced without Cre

    Depositor

    Kazuto Kobayashi

    Article

    Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

    Insert

    ChRWR/Venus
    

    Use

    AAV

    Mutation

    N/A

    Promoter

    EF1a

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1_ 2xFLAG-2xSTREP_BACH1_Y11A

    Plasmid

    #159131

    Purpose

    Expresses BACH1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

    Insert

    BACH1 (BACH1 Human)
    

    Tags

    2X-FLAG _ 2X-STREP

    Expression

    Mammalian

    Mutation

    Y11A

    Promoter

    CMV

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPN260

    Plasmid

    #91581

    Purpose

    Express sgRNA targeting human CLU

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    CLU (CLU Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPN261

    Plasmid

    #91582

    Purpose

    Express sgRNA targeting human CLU

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    CLU (CLU Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. E1b-GFP-Tol2-Gateway mmu SE-Irf2bpl K

    Plasmid

    #89380

    Purpose

    Vector for reporter assay of one mouse super-enhancer region

    Depositor

    Alena Shkumatava

    Article

    Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

    Insert

    Irf2bpl (Irf2bpl Mouse)
    

    Available Since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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