We narrowed to 17,638 results for: URE

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pMSCVpuro-eGFP-hcGAS

Plasmid

#108674

Purpose

For production of retrovirus and transduction of mammalian cells, allowing expression of N-terminally eGFP-tagged human cGAS

Depositor

Andrew Jackson, Martin Reijns

Article

Mackenzie et al Nature. 2017 Aug 24;548(7668):461-465. d

Insert

eGFP-cGAS (CGAS Human)

Use

Retroviral

Promoter

LTR

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHAhFMRPiso1

Plasmid

#48690

Purpose

Expresses hFMRP iso 1

Depositor

Thomas Tuschl

Article

Ascano M et al Nature. 2012 Dec 20;492(7429):382-6. doi

Insert

FlagHA FMR1 Iso 1 (FMR1 Human)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

May 19, 2014

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHAhFXR1

Plasmid

#48694

Purpose

Expresses hFXR1

Depositor

Thomas Tuschl

Article

Ascano M et al Nature. 2012 Dec 20;492(7429):382-6. doi

Insert

FlagHA FXR1 (isoform b) (FXR1 Human)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

June 13, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX_hDDX5_FL_wt

Plasmid

#88871

Purpose

inducible expression of GST-DDX5 in bacteria

Depositor

Dan Littman

Article

Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

Insert

DDX5 (DDX5 Human)

Tags

GST

Expression

Bacterial

Promoter

Tac

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pMThy1.1_hDDX5_FL

Plasmid

#88873

Purpose

constitutive expression of DDX5 in mammalian cells

Depositor

Dan Littman

Article

Huang et al Nature. 2015 Dec 24;528(7583):517-22. do

Insert

DDX5 (DDX5 Human)

Use

Retroviral

Expression

Mammalian

Promoter

MSCV

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCDH1-CMV-HT-LC3-SV40-Hygro

Plasmid

#182045

Purpose

Lentiviral expression vector encoding HaloTag-LC3 for monitoring autophagosome closure

Depositor

Hong-Gang Wang

Article

Takahashi et al J Cell Biol. 2019 Oct 7;218(10):3336-335

Insert

MAP1LC3B (MAP1LC3B Human)

Use

Lentiviral

Tags

HaloTag-TEV

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-eGFP-mcGAS

Plasmid

#108675

Purpose

For production of retrovirus and transduction of mammalian cells, allowing expression of N-terminally eGFP-tagged mouse cGAS

Depositor

Andrew Jackson, Martin Reijns

Article

Mackenzie et al Nature. 2017 Aug 24;548(7668):461-465. d

Insert

eGFP-cGAS (Cgas Mouse)

Use

Retroviral

Promoter

LTR

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGmCherry-gRNA

Plasmid

#87110

Purpose

gRNA cloning vector with CAGmCherry

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCR-blunt-TOPO-FNDC5

Plasmid

#35970

Depositor

Bruce Spiegelman

Article

Bostrom et al Nature. 2012 Jan 11;481(7382):463-8. doi

Insert

fibronectin type III domain containing 5 (Fndc5 Mouse)

Use

Cloning vector

Available Since

April 2, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1_MeCP2(deltaNIC)

Plasmid

#110191

Purpose

Expression of mouse MeCP2 (deltaNIC) in mammalian cells

Depositor

Adrian Bird

Article

Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

Insert

MeCP2_e2 delta NIC (mouse cDNA) - EGFP (Mecp2 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

delta N and C termini. Intervening region replace…

Promoter

CMV

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNAI-GAL4-CREB-S133A

Plasmid

#46770

Purpose

Expression of GAL4 fusion to CREB-S133A

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

GAL4(1-147)-CREB-S133A (Creb1, GAL4 Budding Yeast, Rat)

Tags

GAL4

Expression

Mammalian

Mutation

Changed CREB serine 133 to Alanine

Promoter

CMV

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNAI-GAL4-CREB-S142A

Plasmid

#46771

Purpose

Expression of GAL4 fusion to CREB-S142A

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

GAL4(1-147)-CREB-S142A (Creb1, GAL4 Budding Yeast, Rat)

Tags

GAL4

Expression

Mammalian

Mutation

Changed CREB serine 142 to Alanine

Promoter

CMV

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pelB-MBP-BB0323-ss

Plasmid

#137067

Purpose

E. coli expression clone (T7lac promoter) for mature BB0323 (aa 20-377) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Insert

AAC66700.1

Tags

PelB signal peptide + His10 + maltose binding pro…

Expression

Bacterial

Mutation

mature BB0323: lacks the BB0323 signal peptide

Promoter

T7lac

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR-53BP1 1-1972 WT

Plasmid

#53446

Purpose

Recombinational donor/master vector

Depositor

Daniel Durocher

Article

Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

Insert

53BP1 (TP53BP1 Human)

Use

Gateway

Mutation

siRNA resistant and mutations A128V, T720A, A1347…

Available Since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pIRESh-GFPII-SM-Dlk1

Plasmid

#108932

Purpose

GFP-tagged vector expressing full length isoform of Dlk1

Depositor

Anne Ferguson-Smith

Article

Ferron et al Nature. 2011 Jul 20;475(7356):381-5. doi

Insert

Dlk1 (Dlk1 Mouse)

Tags

GFPII

Expression

Mammalian

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-FRT/T0-eGFPnls-53BP1 1220-1631 WT

Plasmid

#60814

Purpose

mammalian expression vector

Depositor

Daniel Durocher

Article

Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

Insert

53BP1 (TP53BP1 Human)

Tags

eGFP-NLS (SV40)

Expression

Mammalian

Available Since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1+_FH-AGO2-5xA

Plasmid

#92007

Purpose

Expresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824A, S828A, T830A, S831A, S834A (5xA)

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA6.2-YFP-CWT

Plasmid

#176689

Purpose

Fluorescent reporter for phagosomal escape of Staphylococcus aureus

Depositor

Martin Fraunholz

Article

Blattner et al PLoS Pathog. 2016 Sep 15;12(9):e1005857.

Insert

cell wall-targeting domain of Lysostaphin

Tags

YFP

Expression

Mammalian

Mutation

deletion of protase domain of Lysostaphin

Promoter

CMV

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+_FH-AGO2-5xE

Plasmid

#92008

Purpose

Expresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824E, S828E, T830E, S831E, S834E (5xE)

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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shRNA Caspase 1 (pSicoR mCherry)

Plasmid

#53575

Purpose

shRNA against human pro-caspase 1

Depositor

Warner Greene

Article

Doitsh et al Nature. 2014 Jan 23;505(7484):509-14. do

Insert

shRNA Caspase 1 (CASP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; EF1a for mCherry

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only
pBKWH-p53

Plasmid

#133900

Purpose

Positive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWH

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Insert

p53 (Trp53 Mouse)

Expression

Yeast

Promoter

T7

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-Ago2

Plasmid

#72207

Purpose

Mammalian expression of full-length human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHAhFMRPiso1I304N

Plasmid

#48692

Purpose

Expresses hFMRP iso 1 I304N

Depositor

Thomas Tuschl

Article

Ascano M et al Nature. 2012 Dec 20;492(7429):382-6. doi

Insert

FlagHA FMR1 Iso 1 I304N (FMR1 Human)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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BasiginCD4d3+4-Comp-His-Stop

Plasmid

#36147

Purpose

EXPRESs plasmid for human erythrocyte encoding BSG/EMMPRIN/CD147 and CD4d3+4-Comp-His-Stop pentamer

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Basigin (BSG Human)

Tags

His tag, pentamerization domain of rat cartilagin…

Expression

Mammalian

Promoter

CMV

Available Since

May 14, 2012

Availability

Academic Institutions and Nonprofits only

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pMSCV-MAP3K2

Plasmid

#85652

Purpose

retroviral expression of human MAP3K2

Depositor

Julien Sage

Article

Mazur et al Nature. 2014 Jun 12;510(7504):283-7. doi

Insert

MAP3K2 (MAP3K2 Human)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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AT5G63930 X059_pECIA2

Plasmid

#115116

Purpose

Bait vector AT5G63930 X059_pECIA2 should be used with prey vector AT5G63930 X059_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G63930 (AT5G63930 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT1G17230 X074_pECIA2

Plasmid

#115096

Purpose

Bait vector AT1G17230 X074_pECIA2 should be used with prey vector AT1G17230 X074_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G17230 (AT1G17230 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AT2G33170 X049_pECIA2

Plasmid

#115101

Purpose

Bait vector AT2G33170 X049_pECIA2 should be used with prey vector AT2G33170 X049_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT2G33170 (AT2G33170 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT1G74360 X038_pECIA2

Plasmid

#115080

Purpose

Bait vector AT1G74360 X038_pECIA2 should be used with prey vector AT1G74360 X038_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G74360 (AT1G74360 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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AT5G63930 X059_pECIA14

Plasmid

#114916

Purpose

Prey vector AT5G63930 X059_pECIA14 should be used with bait vector AT5G63930 X059_pECIA2.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G63930 (AT5G63930 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge