We narrowed to 13,546 results for: GAN

1. pLenti-xBE4Gam-P2A-Puro

   Plasmid

   #112676

   Purpose

   Lentivirus for constitutive expression of xCas9(3.7)-BE4Gam in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xCas9(3.7)-BE4Gam
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

   Promoter

   EF1s

   Available Since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCOLADuet-1_nsp10 (SARS-CoV-2)

   Plasmid

   #169158

   Purpose

   To express SARS-CoV-2 nsp10 in E. coli

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   nsp10 (ORF1ab SARS-CoV-2)
   

   Tags

   Methionine

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T7

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-xBE3-P2A-Puro

   Plasmid

   #110870

   Purpose

   Lentiviral vector for constitutive expression of xCas9(3.7)-BE3 in mammalian cells

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xCas9(3.7)-BE3
   

   Use

   Lentiviral

   Mutation

   D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG-HA-FbxO11-Jeff-pcDNA3.1-

   Plasmid

   #52503

   Purpose

   expresses human FbxO11-Jeff mutant in mammalian cells with FLAG-HA tag at N-terminus

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Insert

   FbxO11 (FBXO11 Human)
   

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   Q491L (Jeff)

   Promoter

   CMV

   Available Since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-VQRRA-P2A-GFP-PGK-Puro

   Plasmid

   #110864

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VQRRA-P2A-GFP in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VQRRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Mutation

   D1135V, R1335Q, T1337R and NLS sequence at the N-…

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJJR50

   Plasmid

   #75026

   Purpose

   U6 promoter driven flipped + extended sgRNA expression vector

   Depositor

   Mike Boxem

   Article

   Waaijers et al BMC Biol. 2016 Aug 9;14:66. doi: 10.1186

   Insert

   guide RNA, flipped and extended version
   

   Use

   CRISPR

   Promoter

   R07E5.16 (U6)

   Available Since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-VRERRA-P2A-GFP-PGK-Puro

   Plasmid

   #110865

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VRERRA-P2A-GFP in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VRERRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Mutation

   D1135V, G1218R, R1335E, T1337R, and NLS sequence …

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS2+8-CYP2A6(C14A, C82A, C439A)-flag-tev-halo-his

   Plasmid

   #227164

   Purpose

   For T-REX experiments of CYP2A6 C14A, C82A, and C439A triple mutant. Almost no sensing ability to reactive electrophilic species (RES).

   Depositor

   Yimon Aye

   Article

   Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

   Insert

   CYP2A6 (CYP2A6 Human)
   

   Tags

   Flag, HaloTag, and His6

   Expression

   Mammalian

   Mutation

   changed cysteine 14, cysteine 82, and cysteine 43…

   Promoter

   CMV and SP6

   Available Since

   Aug. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3-pSMANTIS_SMAD-FOS_Mutant

   Plasmid

   #194188

   Purpose

   Includes the promoter (1kb) of SMTS with mutated SMAD/FOS binding site

   Depositor

   Matthias Leisegang, Ralf Brandes

   Article

   Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

   Insert

   SMANTIS promoter (1kb) (SMANTIS Human)
   

   Use

   Luciferase

   Mutation

   mutated SMAD/FOS Site, Region 3-15nt of 1000nt

   Promoter

   SMANTIS promoter (1kb), mutated

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL3-pSMANTIS_STAT1-2_Mutant

    Plasmid

    #194189

    Purpose

    Includes the promoter (1kb) of SMTS with mutated STAT1/2 binding site

    Depositor

    Matthias Leisegang, Ralf Brandes

    Article

    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

    Insert

    SMANTIS promoter (1kb) (SMANTIS Human)
    

    Use

    Luciferase

    Mutation

    mutated STAT1/2 site, Region 653-673nt of 1000nt

    Promoter

    SMANTIS promoter (1kb), mutated

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-VRER-P2A-GFP-PGK-Puro

    Plasmid

    #110862

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VRER-P2A-GFP (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VRER
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Mutation

    D1135V, G1218R, R1335E, T1337R

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-HF1-P2A-GFP-PGK-Puro

    Plasmid

    #110863

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1-P2A-GFP (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-xF2X-P2A-Puro

    Plasmid

    #110873

    Purpose

    Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    xFNLS-2X(3.7)
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Mutation

    D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. His-PHD2-181-426

    Plasmid

    #223551

    Purpose

    His-tagged human PHD2 catalytic domain with TEV cleavage site between His and PHD2 for PHD2 purification

    Depositor

    Somshuvra Mukhopadhyay

    Article

    Gurol et al Proc Natl Acad Sci U S A. 2024 Jun 25;12

    Insert

    PHD2 (EGLN1 Human)
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-180.

    Promoter

    trc

    Available Since

    Sept. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899,884 (All)

    Plasmid

    #194190

    Purpose

    Includes the promoter (1kb) of SMTS with 5 mutated KLF binding sites (positions 449,548,732,899,884)

    Depositor

    Matthias Leisegang, Ralf Brandes

    Article

    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

    Insert

    SMANTIS promoter (1kb) (SMANTIS Human)
    

    Use

    Luciferase

    Mutation

    5 mutated KLF binding sites (positions 449,548,73…

    Promoter

    SMANTIS promoter (1kb), mutated

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL3-pSMANTIS_1kb_KLF2mut 899

    Plasmid

    #194193

    Purpose

    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 899)

    Depositor

    Matthias Leisegang, Ralf Brandes

    Article

    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

    Insert

    SMANTIS promoter (1kb) (SMANTIS Human)
    

    Use

    Luciferase

    Mutation

    mutated KLF binding sites (position 899)

    Promoter

    SMANTIS promoter (1kb), mutated

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL3-pSMANTIS_1kb_KLF2mut 732

    Plasmid

    #194195

    Purpose

    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 732)

    Depositor

    Matthias Leisegang, Ralf Brandes

    Article

    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

    Insert

    SMANTIS promoter (1kb) (SMANTIS Human)
    

    Use

    Luciferase

    Mutation

    mutated KLF binding sites (position 732)

    Promoter

    SMANTIS promoter (1kb), mutated

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL3-pSMANTIS_1kb_KLF2mut 884

    Plasmid

    #194196

    Purpose

    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 884)

    Depositor

    Matthias Leisegang, Ralf Brandes

    Article

    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

    Insert

    SMANTIS promoter (1kb) (SMANTIS Human)
    

    Use

    Luciferase

    Mutation

    mutated KLF binding sites (position 884)

    Promoter

    SMANTIS promoter (1kb), mutated

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL3-pSMANTIS_1kb_KLF2mut 449

    Plasmid

    #194197

    Purpose

    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 449)

    Depositor

    Matthias Leisegang, Ralf Brandes

    Article

    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

    Insert

    SMANTIS promoter (1kb) (SMANTIS Human)
    

    Use

    Luciferase

    Mutation

    mutated KLF binding sites (position 449)

    Promoter

    SMANTIS promoter (1kb), mutated

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899 (Quin)

    Plasmid

    #194191

    Purpose

    Includes the promoter (1kb) of SMTS with 4 mutated KLF binding sites (positions 449,548,732,899)

    Depositor

    Matthias Leisegang, Ralf Brandes

    Article

    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

    Insert

    SMANTIS promoter (1kb) (SMANTIS Human)
    

    Use

    Luciferase

    Mutation

    4 mutated KLF binding sites (positions 449,548,73…

    Promoter

    SMANTIS promoter (1kb), mutated

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge