We narrowed to 6,166 results for: cas9 expression plasmid

1. pAP608-1

   Plasmid

   #99484

   Purpose

   H2B::linker::GFP (contain 3 introns)::3XFlag::TEV::Myc::tbb-2 3UTR

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Methods. 2017 May 15;121-122:86-93. doi:

   Insert

   H2B::linker::GFP with introns::3XFlag::TEV::Myc::tbb-2 3UTR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDD401

   Plasmid

   #91828

   Purpose

   Pmyo-2::GFP FP-slot donor for the SapTrap cloning system

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   Pmyo-2::GFP
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Nucleotide 489 of the myo-2 promoter was changed …

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWR95(URA3)

   Plasmid

   #203917

   Purpose

   BLINCAR plasmid for gene deletion, URA3 target

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Inserts

   URA3 upstream homology
   URA3 downstream homology
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWR95

   Plasmid

   #203916

   Purpose

   BLINCAR plasmid for gene deletion, no genomic target

   Depositor

   J. Brian Robertson

   Article

   Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

   Insert

   modified MCS
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAS_gpyrG2

   Plasmid

   #90277

   Purpose

   (also pMST620-BB3_gpyrG2_cas9) CRISPR/Cas9 plasmid with gRNA for site pyrG2, Cas9

   Depositor

   Michael Sauer

   Article

   Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

   Insert

   gRNA (pyrg2)
   

   Use

   A. niger

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAP1071-2

   Plasmid

   #99502

   Purpose

   3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3utr::linker::TEV

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Methods. 2017 May 15;121-122:86-93. doi:

   Insert

   3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3’utr::linker::TEV
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. U6-(BbsI)sgRNA_CAG-Venus-bpA

   Plasmid

   #86985

   Purpose

   For cloning and expression of sgRNA together with Venus expression

   Depositor

   Ralf Kuehn

   Article

   Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

   Insert

   Venus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Venus GFP variant

   Promoter

   CAG

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMVV205

   Plasmid

   #215342

   Purpose

   Recombineering plasmid with constitutively expressed cas9 and the araBAD promoter expressing αβγ on a BBR1 backbone

   Depositor

   Brian Pfleger

   Article

   Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

   Insert

   Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23105

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIK127

   Plasmid

   #65631

   Purpose

   Expresses gfp::h2b in C. elegans cells from the ubiquitous eef-1A.1 (eft-3) promoter.

   Depositor

   Iskra Katic

   Article

   Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

   Insert

   Peft-3::gfp::h2b::tbb-2 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FASTHDR-Cterm-mClover3-Puromycin

    Plasmid

    #167205

    Purpose

    Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mClover3 and selection with Puromycin

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    mClover3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. FASTHDR-Cterm-HaloTag-Puromycin

    Plasmid

    #167208

    Purpose

    Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with HaloTag and selection with Puromycin

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. FASTHDR-Cterm-mRuby3-Zeo

    Plasmid

    #167206

    Purpose

    Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mRuby3 and selection with Zeocin

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. FASTHDR-Cterm-mTagBFP2-Blasticidin

    Plasmid

    #167207

    Purpose

    Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mTagBFP2 and selection with Blasticidin

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. EGFP gRNA (BRDN0000563266)

    Plasmid

    #80036

    Purpose

    3rd generation lentiviral gRNA plasmid targeting EGFP

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. EGFP gRNA (BRDN0000561167)

    Plasmid

    #80034

    Purpose

    3rd generation lentiviral gRNA plasmid targeting EGFP

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EGFP gRNA (BRDN0000562805)

    Plasmid

    #80035

    Purpose

    3rd generation lentiviral gRNA plasmid targeting EGFP

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FASTHDR-Cterm-NanoLuc-Puromycin

    Plasmid

    #167209

    Purpose

    Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with NanoLuc and selection with Puromycin

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    NanoLuc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ADCK4 gRNA (BRDN0001145019)

    Plasmid

    #76560

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ADCK4

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ADCK4
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ADCK3 gRNA (BRDN0001147832)

    Plasmid

    #77321

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ADCK3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ADCK3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ADCK3 gRNA (BRDN0001146491)

    Plasmid

    #77323

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ADCK3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ADCK3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge