We narrowed to 3,788 results for: golden gate

1. Tad-CBE2.4

   Plasmid

   #235055

   Purpose

   Expresses Tad-CBE2.4 in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE2.4-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, SV40NLS and bpNLS

   Expression

   Mammalian

   Available Since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tad-CBE3.6

   Plasmid

   #235058

   Purpose

   Expresses Tad-CBE3.6 in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE3.6-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, SV40NLS and bpNLS

   Expression

   Mammalian

   Available Since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCHW91013

   Plasmid

   #222450

   Purpose

   Level 1, Position 3 containing CBS4-miniDFR::RUBY::OCS.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Insert

   CBS4-miniDFR::RUBY::OCS
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   no

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJPC055

   Plasmid

   #234536

   Purpose

   TRV2 expressing Cre

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

   Insert

   Cre recombinase
   

   Use

   Recombinase

   Expression

   Plant

   Promoter

   CaMV 35S

   Available Since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPOP-BsmBI

   Plasmid

   #241281

   Purpose

   Cloning gene libraries from oligonucleotide pools

   Depositor

   Sean Cutler

   Article

   Dvir et al Proc Natl Acad Sci U S A. 2025 Aug 5;122

   Type

   Empty backbone

   Use

   Other

   Available Since

   Aug. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPOP-BbsI

   Plasmid

   #241282

   Purpose

   Cloning gene libraries from oligonucleotide pools

   Depositor

   Sean Cutler

   Article

   Dvir et al Proc Natl Acad Sci U S A. 2025 Aug 5;122

   Type

   Empty backbone

   Use

   Other

   Available Since

   Aug. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGG-BC-TurboID-GOI

   Plasmid

   #209390

   Purpose

   Golden Gate compatible plasmid for TurboID-based proximity labelling experiment in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Type

   Empty backbone

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGG-GOI-DE-TurboID

   Plasmid

   #209388

   Purpose

   Golden Gate compatible plasmid for TurboID-based proximity labelling experiment in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Type

   Empty backbone

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ143

   Plasmid

   #69295

   Purpose

   Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Nov. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. sgRNA_expression_vector

    Plasmid

    #210212

    Purpose

    An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.

    Depositor

    Albert Jeltsch

    Article

    Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi

    Type

    Empty backbone

    Expression

    Bacterial and Mammalian

    Promoter

    U6

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEC2483 (pSpy1C)

    Plasmid

    #220248

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expression

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCHW111040

    Plasmid

    #222451

    Purpose

    Level 1, Position 3 containing CBS4-CMV::RUBY::OCS.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Insert

    CBS4-CMV1::RUBY::OCS
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    no

    Available Since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. BCJJ345

    Plasmid

    #117504

    Purpose

    YAO:Cas9:E9 Golden Gate Level 1 Position 2 reverse

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:tagt:YAO:Cas9-3(intron):E9:ttgc:BpiI
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtYAO

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIVT-Bxb1

    Plasmid

    #232746

    Purpose

    IVT template for making modRNA encoding Bxb1

    Depositor

    Kate Galloway

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    Bxb1
    

    Use

    In vitro transcription

    Tags

    HA and SV40 NLS

    Available Since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. MbCas12a_N1153_GPG_GR2

    Plasmid

    #231578

    Purpose

    Encodes a cortisol inducible variant of MbCas12a

    Depositor

    Dominik Niopek

    Article

    Wolf et al bioRxiv 2024.12.04.626757

    Insert

    MbCas12a with a GR2 insertion behind N1153
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIVT-mGL

    Plasmid

    #232768

    Purpose

    IVT template for making modRNA encoding mGreenLantern

    Depositor

    Kate Galloway

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mGreenLantern
    

    Use

    In vitro transcription

    Available Since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pICSL11015

    Plasmid

    #117499

    Purpose

    FAST-Red selectable marker Golden Gate Level 1 Position 1

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:TGCC:pOLE1:OLE1-RFP:OLE1t:GCAA:BpiI
    

    Tags

    RFP

    Expression

    Plant

    Promoter

    AtOLE1

    Available Since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJJB1577

    Plasmid

    #218619

    Purpose

    Express Pex5, Pex2, Pex17, and Pex10

    Depositor

    John Dueber

    Article

    Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

    Insert

    Pex5
    

    Expression

    Yeast

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. MET-HYGRO

    Plasmid

    #218113

    Purpose

    Integration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.

    Depositor

    Carl Denard

    Article

    Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination

    Plasmid

    #40132

    Purpose

    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (ELD).

    Depositor

    Pawel Pelczar

    Article

    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

    Type

    Empty backbone

    Tags

    FokI nuclease (ELD)

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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