2,226 results

1. pBBK54 pHomL-RPL18Bp-Neon(gfp)-Link-2xPH(PLCd)-SSA1t-HomR (AmpR)

   Plasmid

   #179038

   Purpose

   Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-2xPH(PLCd), a marker for the plasma membrane

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   RPL18Bp-Neon(gfp)-Link-2xPH(PLCd)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pORANGE Tubb3-2xGFP KI

   Plasmid

   #183443

   Purpose

   GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA and 2xGFP donor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOC4 Kcnn2-smFP-myc KI

   Plasmid

   #183438

   Purpose

   FlpON knock-in for SK2-smFP-myc (amino acid position: T571)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA and smFP myc donor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOC2 GFP-Mpp2 KI

   Plasmid

   #183434

   Purpose

   CreOFF knock-in for GFP-MPP2 (amino acid position: A4)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA and GFP donor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pOC5 smFP-HA-Cacna1e KI

   Plasmid

   #183441

   Purpose

   FlpOFF knock-in for smFP-HA-CaV2.3 (amino acid position: G5)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA and smFP HA donor
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pOC2 Gria1-Halo KI

   Plasmid

   #183433

   Purpose

   CreOFF knock-in for GluA1-Halo (amino acid position: stop codon)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA and Halo donor
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pORANGE Tubb3-3xGFP KI

   Plasmid

   #183444

   Purpose

   GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA and 3xGFP donor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pOC2 Tubb3 no donor

   Plasmid

   #183431

   Purpose

   CreOFF gRNA expression for beta3-tubulin (amino acid position: stop codon)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pOC2 GFP-Actb KI

   Plasmid

   #183429

   Purpose

   CreOFF knock-in for GFP-beta-actin (amino acid position: before start codon)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA and GFP donor
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pOC4 Grin1-smFP-V5 KI

    Plasmid

    #183439

    Purpose

    FlpON knock-in for GluN1-smFP-V5 (amino acid position: A20)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and smFP V5 donor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pOC2 Gria1-GFP KI

    Plasmid

    #183430

    Purpose

    CreOFF knock-in for GluA1-GFP (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and GFP donor
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PX552-rScn2a-3xV5-Syn-miRFP670

    Plasmid

    #182566

    Purpose

    For rat Nav1.2 knockin with 3xV5 at the extracellular loop

    Depositor

    James Zhe Liu

    Article

    Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

    Inserts

    rat Scn2a gRNA and 3xV5 donor DNA
    miRFP670
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Syn and U6

    Available since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRB131

    Plasmid

    #180174

    Purpose

    pAAV construct GluN1A714L (shRNA resistant)

    Depositor

    Richard Bergeron

    Article

    Cappelli et al iScience. 2021 Dec 3;25(1):103539. doi:

    Insert

    GluN1 (Grin1 Rat)
    

    Use

    AAV

    Tags

    Phluorin

    Expression

    Mammalian

    Mutation

    A714L

    Promoter

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLIC-His-GBAi W211A

    Plasmid

    #171756

    Purpose

    For the expression of His-GBAi W211A in bacteria

    Depositor

    Mikel Garcia-Marcos

    Article

    Leyme et al Proc Natl Acad Sci U S A. 2017 Nov 28;11

    Insert

    GBAi
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pGEX-4T Doc2beta C2AB(125) H158C

    Plasmid

    #134691

    Purpose

    Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Double C2 protein beta (Doc2b Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    H158C, C145A, C217A, C249A, C290A, C338A, C387A

    Promoter

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX-4T Doc2beta C2AB(125) F222C

    Plasmid

    #134692

    Purpose

    Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Double C2 protein beta (Doc2b Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    F222C, C145A, C217A, C249A, C290A, C338A, C387A

    Promoter

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX-4T Doc2beta C2AB(125) A298C

    Plasmid

    #134693

    Purpose

    Encodes cytoplasmic domain of Doc2beta for bacterial expression, purification, and labeling

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Double C2 protein beta (Doc2b Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    A298C, C145A, C217A, C249A, C290A, C338A, C387A

    Promoter

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX-4T Syt1 C2AB(140) M173C

    Plasmid

    #134696

    Purpose

    Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Synaptotagmin 1 (Syt1 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    M173C, C277A

    Promoter

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX-4T Syt1 C2AB(140) F234C

    Plasmid

    #134697

    Purpose

    Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Synaptotagmin 1 (Syt1 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    F234C, C277A

    Promoter

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX-4T Syt1 C2AB(140) V304C

    Plasmid

    #134698

    Purpose

    Encodes cytoplasmic domain of synaptotagmin-1 for bacterial expression, purification, and labeling

    Depositor

    Edwin Chapman

    Article

    Bradberry et al J Biol Chem. 2019 Jul 12;294(28):10942-1

    Insert

    Synaptotagmin 1 (Syt1 Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    V304C, C277A

    Promoter

    Available since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge