Addgene

We narrowed to 6,446 results for: poly

Showing: 2941 - 2970 of 6446 results

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25Q-ymScarlet

Plasmid

#128561

Purpose

Httex1 (without proline-rich region) fused to ymScarlet

Depositor

Patrick Lajoie

Article

Albakri et al F1000Res. 2018 Aug 10;7:1242. doi: 10.12

Insert

25Q-Httex1 (without proline-rich region) fused to ymSarlet

Use

Tags

ymScarlet

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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Fuw PBRM1 mBD4

Plasmid

#121108

Purpose

conditional expression

Depositor

Emily Dykhuizen

Article

Porter et al J Biol Chem. 2017 Jan 4. pii: jbc.M116.7

Insert

PBRM1 (N601A) (PBRM1 Human)

Use

Lentiviral

Tags

Expression

Mutation

PBRM1 (N601A)

Promoter

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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Fuw PBRM1 mBD5

Plasmid

#121109

Purpose

conditional expression

Depositor

Emily Dykhuizen

Article

Porter et al J Biol Chem. 2017 Jan 4. pii: jbc.M116.7

Insert

PBRM1 (N739A) (PBRM1 Human)

Use

Lentiviral

Tags

Expression

Mutation

PBRM1 (N739A)

Promoter

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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Fuw PBRM1 mBD1

Plasmid

#121105

Purpose

conditional expression

Depositor

Emily Dykhuizen

Article

Porter et al J Biol Chem. 2017 Jan 4. pii: jbc.M116.7

Insert

PBRM1 (Y127A) (PBRM1 Human)

Use

Lentiviral

Tags

Expression

Mutation

PBRM1 (Y127A)

Promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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Fuw PBRM1 mBD3

Plasmid

#121107

Purpose

conditional expression

Depositor

Emily Dykhuizen

Article

Porter et al J Biol Chem. 2017 Jan 4. pii: jbc.M116.7

Insert

PBRM1 (N463A) (PBRM1 Human)

Use

Lentiviral

Tags

Expression

Mutation

PBRM1 (N463A)

Promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Fuw PBRM1 mBD2

Plasmid

#121106

Purpose

conditional expression

Depositor

Emily Dykhuizen

Article

Porter et al J Biol Chem. 2017 Jan 4. pii: jbc.M116.7

Insert

PBRM1 (N263A) (PBRM1 Human)

Use

Lentiviral

Tags

Expression

Mutation

PBRM1 (N263A)

Promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2-amo

Plasmid

#113190

Purpose

Expresses amo in vertebrate cells

Depositor

Craig Montell

Article

Watnick et al Curr Biol. 2003 Dec 16;13(24):2179-84.

Insert

amo (Pkd2 Fly)

Use

In vitro transcription

Tags

Expression

Mammalian

Mutation

Y602H

Promoter

simian CMV IE94

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pKN3097

Plasmid

#113392

Purpose

Galactose-inducible Ty3 with extra PPT- used for random bar code library

Depositor

Suzanne Sandmeyer

Article

Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

Insert

Ty3

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pET30a-RAMA

Plasmid

#112226

Purpose

Expresses RAMA domain from murine MPND

Depositor

Douglas Feldman

Article

Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

Insert

MPND (Mpnd Mouse)

Use

Tags

Flag and HIs S-tag

Expression

Bacterial

Mutation

Promoter

Available since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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pstb-LAFR5-exoY

Plasmid

#86170

Purpose

pstb-LAFR5 with the S. meliloti 1021 exoY fragment from E6 cloned into Pst/Bam

Depositor

Kay Jones

Article

Jones et al J Bacteriol. 2012 Aug;194(16):4322-31. d

Insert

exoY

Use

Tags

Expression

Bacterial

Mutation

Promoter

Smb21651

Available since

Feb. 6, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pRL439

Plasmid

#70258

Purpose

Source of psbA promoter of Amaranthus hybridus, used as a strong promoter of cyanobacterial genes

Depositor

Peter Wolk

Article

Elhai et al Gene. 1988 Aug 15;68(1):119-38.

Insert

psbA promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV-PM-BAF180-SH3

Plasmid

#41933

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BAF180

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only
MSCV-PM-BAF180-SH1

Plasmid

#41931

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BAF180

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only
sugarless cDNA

Plasmid

#42063

Depositor

Norbert Perrimon

Article

Hacker et al Development. 1997 Sep;124(18):3565-73.

Insert

sugarless (sgl Fly)

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 21, 2013

Availability

Academic Institutions and Nonprofits only
pTOPO-rRNA-5'ETS 923 to 1179

Plasmid

#23995

Depositor

Susan Baserga

Article

Gallagher et al Genes Dev. 2004 Oct 15. 18(20):2506-17.

Insert

rRNA 5'ETS ntds 923 to 1179

Use

Bacterial cloning - topo

Tags

Expression

Mutation

None

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only
pTOPO-rRNA-5'ETS 1 to 177

Plasmid

#23993

Depositor

Susan Baserga

Article

Gallagher et al Genes Dev. 2004 Oct 15. 18(20):2506-17.

Insert

rDNA 5'ETS ntds 1 to 177

Use

Bacterial cloning - topo

Tags

Expression

Mutation

None

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only
pTOPO-25S rDNA

Plasmid

#23996

Depositor

Susan Baserga

Article

Gallagher et al Genes Dev. 2004 Oct 15. 18(20):2506-17.

Insert

25S rRNA from +5270 to oligo y

Use

Bacterial cloning - topo

Tags

Expression

Mutation

none

Promoter

Available since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pGMEH-P38-luxAFB-DAS+4

Plasmid

#27064

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-luxA-FLAG-luxB-DAS+4

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pGMCtN-P38-luxAB-DAS+4

Plasmid

#27067

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P#38-luxAB-DAS+4 integrative

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pBP-lldR

Plasmid

#74095

Purpose

L-lactate repressor (LldR) from E. coli MG1655

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

lldR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

None

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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hMTP-flag

Plasmid

#138335

Purpose

Expresses MTP

Depositor

Mahmood Hussain

Article

Rava et al J Biol Chem. 2006 Apr 21;281(16):11019-2

Insert

MTTP (MTTP Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pgRNA

Plasmid

#215560

Purpose

Constitutively expressed sgRNA targeting a desired gene (this case pta), ColE1 origin, KanR

Depositor

Brian Pfleger

Article

Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

Insert

gRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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Ki67-mNeonGreen-IRESpuro2

Plasmid

#183737

Purpose

Expresses Ki67-mNeonGreen

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

MKI67 (MKI67 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 TFEC

Plasmid

#67060

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TFEC (TFEC Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
H2B-mNeonGreen-IRESpuro2

Plasmid

#183745

Purpose

Expresses H2B-mNeonGreen

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

H2B (H2BC16P Human)

Use

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pTU1-A-RFP

Plasmid

#72939

Purpose

Level 1 Destination vector - Position A

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 1 - A site

Use

Synthetic Biology

Tags

Expression

Mutation

Beta-lactamase - G717C (nucleotide) - silent muta…

Promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBP-SJM901

Plasmid

#72966

Purpose

Level 0 - SJM901 promoter (5' CTAT/3' GTAC fusion)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

SJM901 promoter

Use

Synthetic Biology

Tags

Expression

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 TCF12

Plasmid

#67113

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TCF12 (TCF12 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 CYP2A13

Plasmid

#67103

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

CYP2A13 (CYP2A13 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pMSP2

Plasmid

#26282

Depositor

Stephen Sligar

Article

Ritchie et al Methods Enzymol. 2009 . 464():211-31.

Insert

MSP2

Use

Tags

6-His

Expression

Bacterial

Mutation

synthetic gene of dimer of deletion mutant (1-43)…

Promoter

Available since

April 7, 2011

Availability

Academic Institutions and Nonprofits only