We narrowed to 6,648 results for: SIM;

1. pT26CfpSox10G4FER3(#1048)

   Plasmid

   #184073

   Purpose

   cyclofen-inducible GAL4 activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Insert

   Gal4bdFF-ERT2
   

   Use

   Zebrafish transgenesis (blue heart)

   Tags

   Expression

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   Zf-Sox10

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. FusX alphaDdCBE 1397C

   Plasmid

   #221936

   Purpose

   Backbone plasmid for FusX-based assembly of alphaDdCBE arm with DddAtox 1397C

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   3xFLAG

   Expression

   Mutation

   TALE: Q231R, W232G, S233A

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. FusX alphaDdCBE 1397N

   Plasmid

   #221937

   Purpose

   Backbone plasmid for FusX-based assembly of alphaDdCBE arm with DddAtox 1397N

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   3xFLAG

   Expression

   Mutation

   TALE: Q231R, W232G, S233A

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. FusX DdCBE DddA6-11 1397C

   Plasmid

   #221933

   Purpose

   Backbone plasmid for FusX-based assembly of DdCBE arm with DddA6/11 1397C

   Depositor

   Stephen Ekker

   Article

   Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   3xFLAG

   Expression

   Mutation

   DddA: T1413I

   Promoter

   Available since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMOD4 galK-G

   Plasmid

   #19182

   Depositor

   Al Fisher

   Article

   Zhang et al BMC Dev Biol. 2008 Dec 30;8(1):119.

   Insert

   galK (galK E.coli)
   

   Use

   Recombineering

   Tags

   50 nucleotides of FLAG-GFP

   Expression

   Mutation

   Promoter

   Available since

   Jan. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYS1

   Plasmid

   #117488

   Purpose

   Generation of unmarked gene deletions in Mycobacterium smegmatis

   Depositor

   Eyal Gur

   Article

   Shenkerman et al Gene. 2014 Jan 1;533(1):374-8. doi: 10.1

   Insert

   Che9c 60–61, sacB, aph
   

   Use

   Deletion generation

   Tags

   Expression

   Mutation

   Promoter

   Acetamide

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCB301TOR-GFP

   Plasmid

   #164799

   Purpose

   Expression of GFP in oomycetes using Agrobacterium-mediated transformation

   Depositor

   Miaoying Tian

   Article

   Wu et al BMC Microbiol. 2016 Sep 6;16(1):204. doi

   Insert

   GFP
   

   Use

   Oomycete expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKEW394_AcrIIA14

   Plasmid

   #138283

   Purpose

   Expression of AcrIIA14 protein

   Depositor

   Jennifer Doudna

   Article

   Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

   Insert

   AcrIIA14
   

   Use

   CRISPR

   Tags

   SFGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSANG10-3F

   Plasmid

   #39264

   Depositor

   John McCafferty

   Article

   Martin et al BMC Biotechnol. 2006 Dec 7;6:46.

   Type

   Empty backbone

   Use

   Bacterial expression, bacterial expression of re…

   Tags

   6xHis, pelB leader, and tri-FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. T7-pAc5.1 mCherry EcoRV

    Plasmid

    #206490

    Purpose

    Schneider cell imaging

    Depositor

    Anne Ephrussi

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Type

    Empty backbone

    Use

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAP28

    Plasmid

    #194707

    Purpose

    Linker::3xFlag::mScarlet::HAtag::Linker

    Depositor

    Alexandre Paix

    Article

    Seleit et al Elife. 2021 Dec 6;10. pii: 75050. doi: 1

    Insert

    Linker::3xFlag::mScarlet::HAtag::Linker
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKHR4

    Plasmid

    #74592

    Purpose

    an ampR plasmid built from pBluescript: the multiple cloning site (mcs) flanked by inverted head-to-head oriented I-SceI sites. Genbank accession number is KU144822.

    Depositor

    David Grunwald

    Article

    Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAP27

    Plasmid

    #194706

    Purpose

    Linker::3xFlag::mNeonGreen::HAtag::Linker

    Depositor

    Alexandre Paix

    Article

    Seleit et al Elife. 2021 Dec 6;10. pii: 75050. doi: 1

    Insert

    Linker::3xFlag::mNeonGreen::HAtag::Linker
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDO23A

    Plasmid

    #27367

    Depositor

    Dirk Schnappinger

    Article

    Blumenthal et al PLoS One. 2010 Dec 22;5(12):e15667. doi:

    Insert

    pUC/ori, bla
    

    Use

    Gateway donor vector

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    Available since

    May 11, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBabe GFP Small T antigen

    Plasmid

    #10673

    Depositor

    William Hahn

    Article

    Boehm et al Mol Cell Biol. 2005 Aug . 25(15):6464-74

    Insert

    SV40 small T Antigen (SV40gp7 simian virus)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

16. pSG5 E107K T Ag

    Plasmid

    #133710

    Purpose

    Large T antigen mutant form in pSG5 vector for mammalian expression.

    Depositor

    James A. DeCaprio

    Article

    Zalvide et al Mol Cell Biol. 1995 Oct . 15(10):5800-10

    Insert

    SV40gp6 (SV40gp6 SV40)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E107K

    Promoter

    Available since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGFP CrkII

    Plasmid

    #50730

    Purpose

    mammalian expression of GFP-CrkII. Note- This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

    Depositor

    Kenneth Yamada

    Insert

    CrkII (CRK Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pNIC-TolB-NHSTIIT-GG

    Plasmid

    #215792

    Purpose

    Cloning of target gene with N terminal TolB SRP pathway signal peptide N terminal His6 Twin Strep tag and TEV cleavage

    Depositor

    ASAP Discovery, Frank von Delft

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pNIC-PelB-NH10T-AVITAG-GG

    Plasmid

    #215793

    Purpose

    Cloning of target gene with N terminal PelB SEC pathway signal peptide His10 and AVITAG and TEV cleavage

    Depositor

    ASAP Discovery, Frank von Delft

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pNIC-HIS6-GST-TEV-AVITAG-GG

    Plasmid

    #215809

    Purpose

    Cloning of target gene with N terminal His6 GST tag and TEV cleavage non cleavable N-terminal avitag

    Depositor

    ASAP Discovery, Frank von Delft

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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