We narrowed to 3,663 results for: golden gate

1. L0-I18 Ub-Arg GG1-GG2

   Plasmid

   #195899

   Purpose

   Level 0 Ubiquitin-Arg tag, protein degration tag with GG1 GG2 golden gate linkers

   Depositor

   Jennifer Nemhauser

   Article

   Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

   Insert

   Ub-Arg tag
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPD355 VPR-FRB (NLS)

   Plasmid

   #138847

   Purpose

   Constitutive expression of VPR-FRB under the CMV promoter.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   VPR-FRB
   

   Use

   Synthetic Biology

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. L0-I39 PhiC31-Intein GG1-GG4

   Plasmid

   #195905

   Purpose

   Level 0 PhiC31-intein(DnaE-Npu) with GG1 and GG4 golden gate linkers

   Depositor

   Jennifer Nemhauser

   Article

   Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

   Insert

   PhiC31-intein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. L0-I40 Intein-PhiC31 GG1-GG4

   Plasmid

   #195906

   Purpose

   Level 0 Intein(DnaE-Ssp)-PhiC31 with GG1 and GG4 golden gate linkers

   Depositor

   Jennifer Nemhauser

   Article

   Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

   Insert

   Intein-PhiC31
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. BCJJ340

   Plasmid

   #117502

   Purpose

   UBI10:Cas9:E9 Golden Gate Level 1 Position 2

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BpiI:GCAA:UBI10:Cas9-3(intron):E9:ACTA:BpiI
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtUBI10

   Available since

   Oct. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGGF008

   Plasmid

   #48848

   Purpose

   Provides a BASTA resistance cassette as GreenGate module.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Insert

   pNOS::BastaR::tNOS
   

   Use

   Golden gate compatible cloning vector

   Tags

   Expression

   Mutation

   chi sequence in BastaR removed by silent substitu…

   Promoter

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLY134

   Plasmid

   #130962

   Purpose

   Engineered sgRNA-LEB5 generator including Anderson promoter J23100 for golden gate assembly

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Insert

   sgRNA-LEB5
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Anderson promoter J23100

   Available since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEPOR1CB0058

   Plasmid

   #117539

   Purpose

   Level1 Golden Gate Cassette: sgRNA cassette for SaCas9, targeting NbPDS gene in N. benthamiana

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   sgRNA (SaCas9)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pZA55

   Plasmid

   #174768

   Purpose

   Entry clone for Golden Gate assembly

   Depositor

   Sophien Kamoun

   Article

   Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

   Insert

   GG_NbZAR1syn-L17E without STOP codon
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   L17E

   Promoter

   Available since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pZA56

    Plasmid

    #174769

    Purpose

    Entry clone for Golden Gate assembly

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    GG_NbZAR1syn-L17E/D481V without STOP codon
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    L17E/D481V

    Promoter

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pZA54

    Plasmid

    #174767

    Purpose

    Entry clone for Golden Gate assembly

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    GG_NbZAR1syn-D481V without STOP codon
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    D481V

    Promoter

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pZA53

    Plasmid

    #174766

    Purpose

    Entry clone for Golden Gate assembly

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    GG_NbZAR1syn without STOP codon
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAS1_4xh7SKPylT_EF1_BsmBI_Intein_DD

    Plasmid

    #162810

    Purpose

    STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression

    Depositor

    Simon Elsaesser

    Article

    Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

    Insert

    BsmBI_Intein_DD
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMH184

    Plasmid

    #122857

    Purpose

    MS2-modified guide RNA targeting the FT promoter with GreenGate E-F flanking sequences

    Depositor

    Markus Schmid

    Article

    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

    Insert

    MS2-modified sgRNA targeting the Arabidopsis FT promoter
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEPOR0SP0007

    Plasmid

    #117534

    Purpose

    Level0 Golden Gate part: CDS, LbCas12a

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    LbCas12a (with stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGGD007

    Plasmid

    #48837

    Purpose

    Provides linker:nuclear localization signal as GreenGate C-terminal tag module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    linker:nuclear localization signal
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEPOR0SP0021

    Plasmid

    #117532

    Purpose

    Level0 Golden Gate part: CDS, SaCas9 (no stop codon)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SaCas9 (no stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEPOR0SP0016

    Plasmid

    #117526

    Purpose

    Level0 Golden Gate part: CDS, Sp-eCas9 1.0

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    Sp-eCas9 1.0 (with stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    K810A, K1003A, R1060A

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. p0AmpCD

    Plasmid

    #237276

    Purpose

    Empty vector for L0 TypeIIS

    Depositor

    Eric Young

    Article

    Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p0AmpAB

    Plasmid

    #237274

    Purpose

    Empty vector for L0 TypeIIS

    Depositor

    Eric Young

    Article

    Tobin et al Appl Microbiol Biotechnol. 2024 Dec 28;1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge