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We narrowed to 653 results for: EPO

Showing: 1 - 10 of 653 results

  1. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...submit deposits with more than 10 plasmids is to use our deposit spreadsheet?  Our plasmid deposit process...Online Deposit” and “Download Deposit Spreadsheet” (see Figure 1). Please read our “How to Deposit Your ...Figure 1: Begin the deposit process by clicking “Submit Online Deposit”or “Download Deposit Spreadsheet.” If...Start Your Deposit Deposit FAQ Which Plasmids Should I Deposit? ... If you’re depositing 10 or more plasmids, the Deposit Spreadsheet is the quickest option. Simply download... need to deposit a collection that is greater than 75 plasmids, please contact us at deposit@addgene.org....org. These deposits are considered large deposits and are exceptions to our standard workflow.  Table...

  2. 2022 Depositor Week

    Type
    Blog Post
    ... researchers who deposit with us!  This year alone, we had 12,427 plasmids deposited with us. We cannot...Wednesday: 2022 Blue Flame Depositors Hall of Fame  Thursday: 8 Great Depositor Research Stories   Friday...to all our depositors: thank you so much!!!  (A special shout-out to those who are depositing their data...Hello and welcome to Depositor Week 2022! It’s that special time of year where we Addgenies use our social...we’re certainly going to try!      Here’s our Depositor Week schedule:  Monday: Thank you post (this is...Friday: Specialized Depositor Shout-Out  We'll also be doing giveaways on Twitter, so keep an eye out there...

  3. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    ...detectable by two different tags: a reporter and an HA tag. The reporter comes in three varieties: HRP, Spaghetti...independent of the reporter. The only caveat is that HA staining is weaker than reporter staining. Picking...Picking the right reporter for your RANBody While all three reporters stain cultured cells and tissue sections...Monster, and Chicken IgY Domain RANbody Reporters. Reporter Detection Method Uses Pros Cons Antigens...genetically tagged and instead are conjugated to reporters (i.e. fluorescent dyes, HRP, etc. ) after purification... is a RANbody? A RANbody is a fusion between a reporter and a nanobody that’s encoded on a plasmid. A ...sections, some reporters are better suited for particular applications. Read on to learn more about each...

  4. 8 Great Depositor Research Stories

    Type
    Blog Post
    ...This Depositor Week, we're highlighting eight amazing posts on research done in our depositor labs. Some...written by Addgenies and some are written by the depositors themselves. All of them have cool new tools, ...scientists to use. As always, a big thank you to our depositors for sharing your science with us! Corralling ...introduced us to RUBY-red, a bright red pigment reporter for plants.  Cas7-11 and PASTE: Omar Abudayyeh...one of our favorite posts from 2022! Wow! Our depositors do some really incredible science - I hope you've...

  5. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    ...notes) Any Data repository Only accepts data for antibodies in Addgene’s repository  IBEX Knowledge-Base...Data repository See blog post mAb3D Atlas Mouse and human brain  IHC Data repository See...there are websites that can help: Antibody data repositories, which share validation data, and antibody search...using antibody search engines and antibody data repositories to find an antibody for your experiment. Search...available antibodies from many vendors, while data repositories share validation and experimental data to help...familiar with in Table 1.  Table 1: Antibody data repositories and search engines Website Targets Application... Notes Antibodypedia Any Any Data repository   YCharOS Human proteins Immunoblot (...

  6. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ... CRISPR reporters in the near future.  SRIRACCHA (stable, but reversible, integrated reporter for assaying...histone)-GFP reporter into the genome of mammalian cells.  Figure 1: The SRIRACCHA reporter construct...Find GEmCherry2 reporter plasmids at Addgene Overall SRIRACHA and GEmCherry2 reporter systems provide ...Recently scientists have created CRISPR fluorescent reporter assays to quantify targeted Cas9 cleavage, and...and non-homologous end joining (NHEJ). In these reporters, editing typically results in a change in the ...this blog we will focus on two fluorescent CRISPR reporter methods, SRIRACCHA and GEMCherry2. We will cover... the Piggybac transposon to stably integrate a reporter gene that contains a puromycin resistance gene...

  7. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...associated with Addgene deposits accumulate more citations than those without Addgene deposits? Even more interestingly...plasmids that the authors deposited with Addgene and those without plasmids deposited with Addgene. Together...plasmids deposited at Addgene – typically about four times as many as papers without plasmids deposited with...Addgene deposits as if they were published in the same journals as those that did make deposits to Addgene...addition to publishing in Science, Professor Zhang deposited the associated plasmids with Addgene. Since then...interestingly, could we tell if depositing a plasmid with Addgene causes a paper to get cited more?   ...scientific articles associated with Addgene plasmid deposits are more highly cited, we went to one of the largest...

  8. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...the deposit process by clicking "Submit Online Deposit" or "Download Deposit Spreadsheet". Start your plasmid...know by contacting deposit@addgene.org. These deposits are considered large deposits and are exceptions... you've deposited with us and distribute them to scientists worldwide. What's more, depositing is free...as a step-by-step guide to the online deposit process. To deposit plasmids online, you will need to create...account, you can begin the deposit process by clicking "Submit Online Deposit" (for online submission) ...) or "Download Deposit Spreadsheet" (for emailed submission) in the Deposit menu: Figure 1: Begin the... plasmid deposit There are two options to begin your plasmid deposit. Figure 2: Choose to submit plasmid...

  9. Depositor Week 2020 is Happening Now. Join us!

    Type
    Blog Post
    ...Tuesday: Depositor features (plasmid deposits and blog posts showcasing the work of depositors) Wednesday...plasmid tools! Thank you depositors! In fact, this year, Addgene depositors quickly stepped up to make...bulk of these plasmid deposits came in this year, there were some plasmids deposited as early as 2006 that... as an example). Depositor Week will be fun for everyone, whether you are a depositor or not. We will ...started from 474 plasmid deposits in 2014 which has grown to ~10,000 plasmid deposits per year in recent years...for some Addgene depositor fun facts for a quick way to grasp the big impacts depositors have on science...your calendars for November 16-20, 2020 for #DepositorWeek on Twitter!  That’s today through Friday. There...

  10. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    ...materials to be deposited into repositories, and financial support to maintain repositories’ capacity for...through a repository, they remain accessible during these times. Centralized reagent repositories, also known...dedicated facilities and manpower that a repository provides. Repositories around the world facilitate the rapid... the scientific community can support repositories. Repositories are essential for the advancement of ...original sources. Figure 1: Biological repositories that distribute mouse strains, plasmids, cell...Culture Collection (ATCC) is a private, nonprofit repository for a variety of materials for life science research... sharing and distribution of cell lines from repositories on opposite sides of the world shows that science...
Showing: 1 - 10 of 653 results