We narrowed to 13 results for: EPO

...and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a, it will release... using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the reaction...following the amplification protocol specified by the depositing lab. After amplification, you’ll need to use ... target sequence, it promiscuously cleaves the reporter as well, and a quantifiable signal is produced...CRISPR 101 eBook (3rd edition) CRISPR software and depositor protocols Video protocol for genomic deletions...NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC Additional Cas9s from...all-in-one CRISPR/Cas9 vector system. Scientific Reports , 4 (1). PMID: 24954249 Shechner, D. M., Hacisuleyman...

...of proteins) and sensors (genetically-encoded reporters of molecular signals). These areas have each since...optogenetics tool. They typically allow the fast depolarization of neurons upon exposure to light through direct...and traffics well to the membrane. Excitatory (Depolarizing) ChR Variants The optogenetic toolbox has been...light-gated, cation channels resulting in excitation (depolarization) of the neuron. Feature enhancements include...

... to amplify the library, please refer to the depositor’s protocol for the best results. For some libraries...die or do not pass selection (in the case of a reporter) Sort “winning” cells Sequence those cells that...Resources Science Exchange Scientist (formally Assay Depot) Resources Find additional resources on our website...

...TALEN Plasmids and Kits , Genome Engineering Kits Reporter Plasmids Used for studying the function of genetic...genetic elements. These plasmids contain a reporter gene (e.g., luciferase or GFP) that offers a readout...Addgene primarily uses for sequencing plasmids deposited at Addgene. The actual process of Illumina NGS...

...about modular cloning (MoClo) and find MoClo kits deposited with Addgene, including the MoClo Toolkit and ...Plasmids with control elements and fluorescent reporters to be used in combination with the MoClo-YTK toolkit...

...control expression of a chemogenetic reporter ensures that the reporter remains silent until a cell or tissue-specific...

...the only type of selectable marker. Fluorescent reporters, such as GFP, are another common selection marker...Some retroviral transfer plasmids that have been deposited with Addgene are self-inactivating (SIN). These...

...in question (see CRISPR protocols from Addgene depositors ). Browse empty gRNA expression vectors . Select...facilitates CRISPR/Cas9-based genome editing. Scientific Reports , 5 , 11315. https://doi.org/10.1038/srep11315 ...

...the only type of selectable marker. Fluorescent reporters, such as GFP, are another common selection marker...the lentiviral transfer plasmids that have been deposited with Addgene are self-inactivating (SIN) plasmids...

... How-to Blog Read about plasmids in Addgene’s repository , and related molecular biology techniques. Topics...

...obtained one of the thousands of entry vectors deposited with Addgene), it can be rapidly shuttled into...

...Adenovirus Vector as a Zika virus vaccine . Scientific Reports, 10 (1), 3613. https://doi.org/10.1038/s41598-020...

...emissions. Cells can also be sorted (separated and deposited into tubes or plate wells) by fluorescence activated...