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We narrowed to 938 results for: Kin

Showing: 1 - 10 of 938 results

  1. Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)

    Type
    Blog Post
    ...less phosphatase-accessible. Kinase Translocation Reporters (KTRs) Kinase Translocation Reporters (KTRs...fluorescent kinase reporters. A KTR is comprised of a fluorescently-tagged substrate for a kinase of interest...proxy for kinase activity.  Advantages of KTRs When compared to FRET reporters for measuring kinase activity...Kinases: they regulate many proteins, with ~1/3 of human proteins predicted to be phosphorylated on at...for regulating signal transduction and measuring kinase activity at the single-cell level can aid in drawing...phenotype. One method for monitoring live single-cell kinase activity is FRET, but FRET reporters are challenging...Covert Lab provides an alternative tool with their Kinase Translocation Reporters (KTRs) whose cellular localization...

  2. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    ...am currently working on projects for the Science Communication function at Addgene. Working a 9-5 As it...one semester (the majority spend two to three) working in your chosen field. This cooperative education...bioengineering student, to Addgene. I am currently working on the QC/QA team as a DNA Sequencing Intern. ...interview was intimidating to me, as I had a hard time thinking about how I could fill this time. I was relieved...environment on my team and at Addgene, I felt comfortable asking questions without fearing they would be disregarded...addition to these core hours, we have flexible working hours. For Addgene that means that it is possible...

  3. Reflections and Looking Ahead

    Type
    Blog Post
    ...story. This year, we made significant strides in making science more accessible and reliable. We continue...flexibility. But this is just the beginning. We’re working toward giving you more choice in how you receive... every breakthrough enabled brings us closer to making an impact on humanity's greatest challenges. Thank...

  4. Working with Nuclear Receptors

    Type
    Blog Post
    ... such as nutritional status. Within the animal kingdom, this information is relayed through factors (both...such as genes involved in metabolism, growth or cytokine action). Primary or core coregulators (those that...cellular roles. In the current phase of NURSA we are seeking to compliment these consortium-generated datasets...with a one-stop shop for plasmids and vectors, and making them available to all researchers for a reasonable...

  5. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    ...side conversations within the team. We are not talking about gossip here – this means encouraging a culture...Connie Cepko to learn about her management style.   Seeking feedback frequently One of the most important paths... only way.   From a colleague: MBWA "Manage By Walking Around" – don't hide in your office. Stop by instead...

  6. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...as-is, saving time and effort but risking the potential of them not working, or spend a few weeks designing...If you’ve ever been looking for just the right CRISPR vectors on Addgene and found instead ones that ... the heavy lifting out of CRISPR vector design, making it easier to get the vectors that are just right...right for your experiment. As always, we recommend checking your cloning via a miniprep and restriction digest...design. Note that you can browse all components by clicking the blue text in the upper right-hand corner.  ...Fragmid website.   If the vector is to your liking, your last step, Step 4, is to give the assembled...six or seven components — in most cases, that's making two or more plasmids — it may be more efficient...

  7. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    ...stories, and other 20th anniversary celebrations, by checking out our blog, social media, newsletter, and website...typically adopted by for-profit companies, while making use of our nonprofit status to achieve mission-...mission and work. In 2024, we will be actively seeking ways to support a global community where every ...the Addgene blog 2022 and 2023: Reflections and Looking Forward...

  8. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...jobs where all I did was public speaking. If you're also nervous, try taking a few deep breaths. Then, identify...breathe!)   Public speaking as a transferable skill The worst thing about doing public speaking as a scientist...Public speaking is not just one of the most common phobias–it’s a key part of being a scientist and an...support for your ideas, invite others into decision making processes, and even interview better! As a scientist...science communicator, I’ve done a lot of public speaking, and I'm happy to share what I've learned along...When my mind went blank, my options were to stop talking until I could present something coherent or to ...me accomplish my goal.   As a scientist, public speaking isn't a performance art. You’re (usually) presenting...

  9. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    ...accomplishments from 2022 and share what we’re looking forward to in 2023 and beyond. Last year, we distributed...rapidly changing environment. In particular, we are working to understand what challenges remain in materials...changing landscape of science. We are therefore embarking on a strategic refresh to understand this new ...help a great number of people. I look forward to working with Addgene colleagues, collaborators, partners...

  10. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...Protein kinases deliver messages to the next members of their pathways, but where the kinases are located...subset of kinases. The Problem:  There is no common method for creating optically controlled kinases. The ...design for creating photoswitchable kinases and use MEK1 as a model kinase. In this system, light is used ...photoswitchable kinases: psMEK2, psRaf1, and psCDK5. MEK2 is a serine/threonine kinase with a similar ...photoswitchable kinases good for? There are a number of potential applications for photoswitchable kinases.  Below...Zhou et al. 1. Testing kinase inhibitors Zhou et al examined the ability of kinase inhibitors to alter Raf1... by intermediate messengers. Optical control of kinase activity can provide greater spatiotemporal resolution...
Showing: 1 - 10 of 938 results