We narrowed to 113 results for: PIP

...interaction partners (PIPs), the FP fragments form the complete FP because the PIPs they’re tethered to ...endogenous PIPs is expressed at increasing levels alongside your BiFC constructs. If your PIPs actually ... YFP) is reunited by the interaction between two PIPs, resulting in a fluorescent readout.   Applications...aberrant subcellular localization of the studied PIPs. The straightforward nature of BiFC also makes it...protein construction Fusing FP fragments to your PIPs requires careful selection of FP fragments and, ...some information about the structures of the tested PIPs. Many split FPs have been validated for BiFC (Kodama...Kodama and Hu, 2012). Having a rough idea of how your PIPs might interact will allow you to determine where...

... et al. (2025). PRISM is part of an integrated pipeline, but individual components can be used separately...

...CRISPResso2 is a robust and easy-to-use computational pipeline for the analysis of genome editing experiments...

...Gadella 61180 pCMU-PMr Plasma membrane (Plants) PIP2a mCherry Maria Harrison 61206 pCMU-PDESr Plasmodesmata...

... monitor the entire pipeline. Benefits of Nextflow for bioinformatics pipelining In many use-cases, including...optimize our pipeline. After reviewing and testing several platforms for engineering pipelines, we selected...management system As novices to creating a robust data pipeline that would be reliable and available for our teams... could orchestrate the steps into a continuous pipeline. We also recognized the software system that we...ours at Addgene, the overall purpose of a data pipeline is to perform several sequential computational...usage of the scripts needs to be customized in the pipeline for the specific needs of the user. Thus, a key..., a workflow management system for scientific pipelines, including bioinformatics. We loved the parallels...

...the larval gut of bagpipe mutants vaguely resemble a bagpipe and historically, bagpipes were made using ... and bagpipe, two genes important for mesoderm differentiation Now, let's turn tinman and bagpipe, two... Cookie Monster, Spaghetti Squash, Tinman, and Bagpipe. How does a gene get its name? Often times, a gene...becomes muscles and connective tissues. tinman and bagpipe are both homeobox genes that are part of a genetic... the dorsal mesoderm, is necessary to activate bagpipe which is localized to segmental clusters of the... to have a heart (Following the Tinman, 2016). Bagpipe on the other hand, is important in the formation...into the muscles of the gut. Embryos that lack bagpipe have severely disrupted visceral mesoderm and subsequently...

...200 ul pipettor is set to 200 ul, wiped down with 70% ethanol, and a box of sterile truncated pipet tips...the pipettor, placed into wells, and partially dislodged from the agarose plug by rapid pipetting, as ...rapidly punch out cell clones with sterile truncated pipet tips. Indeed, an experienced operator can pick 48.... We highly recommend the use of an electronic pipettor with variable spacing (such as the Viaflo from...

...curve, avoid pipetting small volumes (< 2 µL) or volumes too large for a standard micropipette (> 1,000 µL...2. When preparing a dilution series, use a new pipette tip for each step and mix the samples well by vortexing...readout. When preparing a standard curve, change pipette tips and mix thoroughly by inversion or vortexing...series, use consistent dilution factors, change pipette tips regularly, and mix samples very well.  Use...

...into a medium-throughput pipeline for epitope tagging. Using our 96-format pipeline, we can routinely achieve...to contact me at [email protected]. Keep pipetting and happy genome editing! Pooran Dewari is a ... them to study brain tumour biology. When not pipetting, he can be seen daydreaming about sunny days in...Kalantzaki M et al. 2018. An efficient and scalable pipeline for epitope tagging in mammalian stem cells using...