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...This Depositor Week, we're highlighting eight amazing posts on research done in our depositor labs. Some...written by Addgenies and some are written by the depositors themselves. All of them have cool new tools, ...scientists to use. As always, a big thank you to our depositors for sharing your science with us! Corralling ...with Tim Miles: While not technically a research story, I couldn't resist adding in this excellent Q&A ...one of our favorite posts from 2022! Wow! Our depositors do some really incredible science - I hope you've...try in your own work!) If you've got a research story you want to share on our blog, please reach out ...

...fluorescence or voltage indicators.   Viral Vectors and Voltage Indicators As previously mentioned...Voltage indicators are voltmeters for cells! In this blog we will review how these indicators work, their...as they are in vitro. Factors like photobleaching, lack of red-shifted indicators, and brightness of fluorescence...upstream component, the chemical factor, of a naturally occurring voltage indicator. To give an example, a genetically...specific cell types is where viral vectors become invaluable. Viral vectors are mechanisms by which genetic...Resources on the Addgene blog Viral Vectors 101: Chemogenetics Viral Vectors 101: Optogenetics Fluorescent...their use as biological tools. Why Use Voltage Indicators? Electrical signals are integral to many facets...

...of viral vectors here! Need Virus? Check out Addgene's Viral Service! Types of viral vectors Of the many...types, but most vectors are based on adenovirus 5 (Ad5).     Fig. 3 - Viral vector components of...of adenovirus Building viral vectors Addgene has many viral vectors and helper plasmids for all the...NY): Cold Spring Harbor Laboratory Press; 1997. Principles of Retroviral Vector Design. Available from:...adeno-associated virus (AAV). The components of these vectors are also split across several plasmids (often three...called helper plasmids. Below, we’ll breakdown the vector components first by virus and then by function....respectively, act as RNA polymerase II promoter and terminator regions and are slightly different in sequence...

...understand this regulatory network. The transcription atlas and library The transcription factor library ...Transcription factors (TFs) act as the gate keepers for gene expression, turning transcription on and...Computational analysis was used to infer differentiation trajectories and identify both up- and down-regulated pathways...deliver multiple TFs to each cell, allowing for combinatorial overexpression programs on cellular processes...Resources  References Joung J. et al., A transcription factor atlas of directed differentiation. Cell. 2023. ...

...about her mentoring style. Set goals with a mentor: The secret of accountability To make a mentoring relationship...posts in this Mentoring for Scientists series, you have: realized the value of having a mentor, developed...finding mentors and, perhaps, asked someone to support your career development as your mentor. How do ...with a mentor.The responsibility of arranging meetings and setting the direction in a mentoring relationship...An undergraduate can mentor a high school student, a graduate student can mentor an undergraduate, a postdoc...postdoc can mentor a graduate student – you get the idea. There are many advantages to being a Mentor: Learn...want more great advice on mentoring, click below to download the "Mentoring for Scientists" ebook, which...

...opportunities: Try virtual mentoring at Mentornet.net. This is an online database of mentors you can tap for email...someone to be your mentor Mentoring is a “loaded” word.  If you ask someone to be your mentor, it may sound..., get more advice on mentoring: What Makes a Good Mentor? Choosing a Good Mentor for Scientists  ... are potential mentors all around you. This third article in the Addgene Blog Mentoring series will cover...meet, the more potential mentors you will identify. Finding potential mentors is one of the many reasons...tips for finding mentors in this recent blog 5 Strategies for Finding Your Ideal Mentor. One of the best...researcher Connie Cepko to learn about her mentoring style. Mentors at home and away We should all have a ...

...Viral Vectors 101: Viral Applications Viral Vectors 101: Viruses as Biological Tools Viral Vectors 101:...create far safer viral vectors that can be used in BSL-1 or BSL-2 labs. Viral vectors are viruses that have...increase the viral vector’s carrying capacity. In order to allow the viral vectors to reproduce when experimentally...expressed via cells cultured with the vectors. This allows the viral vectors to reproduce only in experimentally...of Viral Vectors 101 posts about each type, so be sure to check our blog, or our Viral Vectors 101 eBook...viral particle is called a virion. Finally, viral vectors, which are used in the lab, are distinct from viruses...copies itself and “splits” into two cells. Viral vectors Viruses are quite appealing as molecular biology...

...lentiviral vectors, how they work, and why you might choose them over integrating lentiviral vectors.  Before...integrase-deficient lentiviral vectors, let’s briefly revisit the basic biology of lentiviral vectors (for a more detailed...lentiviral vectors that are competent for one round of infection (AKA transduction in the viral vector world...life cycle of a lentiviral vector with an integrase-deficient viral vector. Created with BioRender.com...lentiviral vectors? There are several reasons why you might choose an IDLV over a lentiviral vector: 1. No...include detecting vector particle components or performing quantitative PCR titration of vector DNA in samples...Lentiviral vectors have been a staple in molecular biology for over three decades. Widely used across...

...search of phages for therapy Phage Directory started out as a directory of phage researchers and the phages...can be sent out to the directory earlier, but the researchers in the directory are volunteering their ...says. As the labs in the directory step forward to help, the Phage Directory team must decide which labs...Creating a repository of phage data and information Since the inception of Phage Directory ~1 year ago...). The pair quickly teamed up to create Phage Directory in a whirlwind three days in November 2017. ...find much-needed phages for patients.   A phage directory is crucial because it's hard to find the right...a last resort, so a request for phages to the directory does not typically come out until nearly the last...

... Esq and Carl Paratore, MBA, CPA to the Addgene Board of Directors. Katz is the Director of Intellectual...School and Paratore is the General Auditor at Tufts Health Plan. The addition of Katz and Paratore onto the...Learn more about Carl Paratore, MBA, CPA Mr. Paratore serves as the General Auditor at Tufts Health Plan...presence in the biotech sector in both patent law and general life science regulatory law. My role at Suffolk...employment front. Paratore: I work at Tufts Health Plan and my role there is general auditor. I oversee a ...the Board of Directors brings complementary experience and insight to the now 10 member board at Addgene...that Addgene was looking for new board members. Paratore: Kristen Darby, who’s also a board member, reached...