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We narrowed to 11 results for: tet rtTA

Showing: 1 - 10 of 11 results

  1. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...is the tetracycline (Tet)-regulated system. You can choose either the Tet-off system or the Tet-on system... interest (pTet-IRES-EGFP, pPRIME-Tet-GFP-FF3) and one with either tTA or rtTA (FUW-M2rtTA). In this kind...system. In the Tet-off system a Tet-response element (TRE) is placed upstream of the promoter in your transfer...expression. In contrast, in the Tet-on system, tTA has been modified (and renamed rtTA) to bind TRE only in presence...expression kinetics as compared to the Tet-off system. One drawback of the Tet system is that it often requires... In the absence of tetracycline or a derivative such as Doxycycline (Dox), the Tet-controlled transactivator...vector systems containing both your transgene and tTA/rtTA (pINDUCER20, pINDUCER21) have been developed, but...

  2. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...lentivirus for delivering control shRNAs, GFP, and rtTA from the Tet-on system. The first two can serve as controls...experiments, while rtTA can be used to activate expression of a gene under the control of the tet operator. AAV...

  3. Tetracycline Inducible Expression

    Type
    Collection
    ...promoter Tet-On 3G rtTA Bong-Kiun Kaang 20342 FUW-M2rtTA Lentiviral Tet-On vector, expressing rtTA from UbC... of tet-responsive systems: Tet-Off and Tet-On. Read on to learn more about the components of tet systems... center: Tet-Off; right: Tet-On. TRE: Tet response element; tTA: tetracycline-controlled transactivator...expression. Tetracycline On is also known as the rtTA-dependent or tet-inducible system. In effect, Tet-On is...expressed occasionally, use Tet-On: express an rtTA or TetR and include a tet-responsive promoter upstream...expression of EGFP Tet-On 3G rtTA TRE3GS Xiaojun Lian 171123 pLVX-TetOne-Puro-GFP Lentiviral Tet-On vector for...CMV promoter Tet-On 3G rtTA Oskar Laur 96963 pCAG-TetON-3G Mammalian expression of the Tet-On 3G transactivator...

  4. Plasmids 101: Inducible Promoters

    Type
    Blog Post
    ...inducible promoters. The positive inducible tetracycline ON (Tet-On) system, a versatile tool developed for...activation. In this system, the activator rtTA (reverse tetracycline-controlled transactivator) is normally...Addgene.org: Inducible Promoters for Bacteria Tetracycline (Tet) Inducible Systems at Addgene Cre-lox Guide...lot. If you’re looking for high inducibility, the Tet-On system may be a good choice, as it’s documented...require only the addition of an external inducer, like Tet systems, may be activated very rapidly. In contrast...and cannot bind the tetracycline response elements (TRE) in a promoter. Tetracycline and its derivatives...

  6. Lentivirus Plasmids

    Type
    Collection
    ...14749 for Thy1.1 selection. Benoist and Mathis 21915 Tet-pLKO-puro 3rd inducible expression of shRNA; puromycin...EF-1a-driven GFP and shRNA under the control of a tet-responsive H1 promoter Trono 11651 pLVUT-tTR-KRAB...cDNA. Puro selection. Shih 25737 pSLIK-Hygro 3rd Tet-based inducible shRNA or cDNA expression, gateway... other versions of pSLIK. Fraser 15948 pLOVE 3rd Tet inducible gateway destination plasmid for cDNA expression...versions of this plasmid. Root 44012 pInducer20 3rd Tet-inducible lentivirus for ORF expression, multicistronic...See plasmid 21374 for dtomato version. Welm 21916 Tet-pLKO-neo 3rd inducible expression of shRNA; neomycin...Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puro. See article for more versions of this plasmid...

  7. Retrovirus Plasmids

    Type
    Collection
    ...Vignali 27995 TtRMPVIR CMV/MSV Tet-regulated expression of shRNA; expresses rtTA. See Zuber et al. 2011 for...resistance. Hahn 63704 pRetroX GFP T2A Cre CMV/MSV Tetracycline inducible expression of GFP T2A Cre fusion in...

  9. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...has used an inducible MitoTimer reporter using a Tet-on system (pTRE-Tight-MitoTimer) to show its usefulness...cells stably expressing pTRE-Tight-MitoTimer and rtTA. The cells were pulsed with 2µg/ml of Doxycycline...repressor plasmids from Scot Wolfe's lab. These include Tet-inducible CRISPR activators and repressor plasmids...

  10. Cre-lox system

    Type
    Collection
    ... pBS537 tet-hCMV-GFPcre tet inducible Cre-GFP fusion tet-hCMV Mammalian Sauer 11961 pBS596 tet-hCMV-GFPcre...none Mammalian Sauer 11957 pBS595 tet-hCMV-EGFPcre Cre-EGFP fusion Tet inducible Mammalian Sauer 11958 ...Supernova TRE AAV Iwasato 85577 pTC-CMV-Tet CreER expression and tetracyclin-dependent transgene/shRNA expression...ApoE.HCR.hAAT Mammalian Ehmer 85578 pTC-ApoE-Tet CreER expression and tetracyclin-dependent transgene/shRNA expression...tet-hCMV-GFPcre tet inducible Cre-GFP fusion, metallothionein MT-I region including the polyadenylation site and...and several introns tet-hCMV Mammalian Sauer 12168 pMB80 (R26-CreER) Cre-ERT2 with loxp cassette; Targeting...Mammalian Messing 45359 pNK-TGCK Cre-EGFP fusion; Tet inducible - rrTA expression driven by mouse Nkx cardiac...
Showing: 1 - 10 of 11 results