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...detectable by two different tags: a reporter and an HA tag. The reporter comes in three varieties: HRP, Spaghetti...independent of the reporter. The only caveat is that HA staining is weaker than reporter staining. Picking...Picking the right reporter for your RANBody While all three reporters stain cultured cells and tissue sections...Monster, and Chicken IgY Domain RANbody Reporters. Reporter Detection Method Uses Pros Cons Antigens...genetically tagged and instead are conjugated to reporters (i.e. fluorescent dyes, HRP, etc. ) after purification... is a RANbody? A RANbody is a fusion between a reporter and a nanobody that’s encoded on a plasmid. A ...sections, some reporters are better suited for particular applications. Read on to learn more about each...

...protocol and/or import and convert it into RSpace. The reverse flow is also supported, so it’s possible...searchable and shareable, but some ELNs provide better support for reproducibility than others. Unfortunately,...connected approach in action: how the RSpace ELN supports reproducibility RSpace was designed in response... principles have been implemented by providing support for connectivity to external data sources, tools...tool like an ELN. It is nevertheless critically important to be able to conveniently reference specific ...impossible to reproduce. As the graphic depicts, RSpace supports linking to all of the most commonly used online...research captured in the ELN is incomplete. Thus, to support reproducibility an ELN should have integrations...

...tricks will apply to any biology-related customer support interaction. Before contacting customer service...information as possible about the issue. For Addgene support, make sure to mention the plasmid catalog number...have real scientists answering their technical support questions (besides Addgene, I know both Abcam and...question, and now... One of the aspects of customer support that I miss is that you’re never really sure what... response, just keep in mind that our customer support scientists wear many hats at this still relatively... customer support... technical support...

...Axonal Transport It has been demonstrated that certain serotypes can undergo axoplasmic transport. These...of neurons (retrograde transport) or towards the synapse (anterograde transport), which is a possible ...retrograde and anterograde transport, but they also have similar transport characteristics (velocities...strong CNS tropism (4). If axonal transport is not desirable, transport can be reduced by injecting a smaller...and using AAVs have in common? The three most important items when looking for a house and choosing an...this mantra to AAV selection, there are three important factors related to location. Location (1): What...Traveling from the original location - Axonal Transport in the CNS What tissue? What is commonly known...

... CRISPR reporters in the near future.  SRIRACCHA (stable, but reversible, integrated reporter for assaying...histone)-GFP reporter into the genome of mammalian cells.  Figure 1: The SRIRACCHA reporter construct...Find GEmCherry2 reporter plasmids at Addgene Overall SRIRACHA and GEmCherry2 reporter systems provide ...Recently scientists have created CRISPR fluorescent reporter assays to quantify targeted Cas9 cleavage, and...and non-homologous end joining (NHEJ). In these reporters, editing typically results in a change in the ...this blog we will focus on two fluorescent CRISPR reporter methods, SRIRACCHA and GEMCherry2. We will cover... the Piggybac transposon to stably integrate a reporter gene that contains a puromycin resistance gene...

... In this flowchart, you can see the dwindling proportion of AAV particles that make it through each part...expression. The higher the titer, the better. AAV transport and transgene expression It’s clear that we need...be required. For example, retrograde AAV gets transported up the cell’s axon. While the mechanism for this...yet fully understood, we can imagine that this transport is probably not 100% efficient. So the few AAV... particles. Limit tube transfer - the fewer opportunities your viral particles have to stick to your tubes...

...departments from technical support. Technical support careers in brief Technical support careers are open to ...technical support specialist for 4 years. Read on for insights from my time in technical support including... expertise in technical support positions. When applying for technical support positions, you should try...yourself with technical support. Even having personal experience with technical support through the customer...participated in a customer support conversation, just go ahead and contact a technical support team with a question...in a technical support role. What general characteristics do jobs in technical support have? There are... technical support roles. Examples are Technical Service Representative, Technical Support Specialist,...

...but, in today’s post, we’ll discuss some of the important considerations you should think about when developing...ICSS can now be done with neuronal cell-type and temporal precision. In ICSS, a test chamber is used in ...interested in studying a brain region that may be important in memory, an appropriate behavioral test might...more precisely characterized with spatial (CA1), temporal (real-time inhibition), and behavioral (remote...has a bit of a steep learning curve and many opportunities for mistakes. The easiest way to learn optogenetics...differential participation in reward circuitry." Cell reports 8.6 (2014): 1857-1869. Pubmed PMID: 25242321. Pubmed...

... from rare diseases by connecting patients to important resources and promoting research for rare disease...Cancer Alliance Website.  We are proud to provide support to Drs Di Schiavi and Nabavi and will continue ...learn of future rare disease research funding opportunities. References 1. Jones, C., et al. "PP09. 1–2352...Blog Previous BeHeard Awardees Other Efforts to Support Rare Disease Research: IFOPA Donating Plasmids ...

...that company culture and enjoying your job were important parts of team buidling - they relieved some of...

...mentors from your current organization but also important to build relationships outside your organization... get started with mentoring. Read this Catalyst Report “Making Mentoring Work” to learn about what makes... it work by being an educated mentee. Other opportunities: Try virtual mentoring at Mentornet.net. This...make your mentor want to keep working with you is report back with progress. Tell them how their advice ...

... an odd or quirky lab story you’d like to see portrayed in LEGO form, feel free to share with us at BrickingScience...

...the community as a whole could support Addgene as we have been supporting the community. While many were...Zuckerberg Initiative for supporting Addgene by providing a generous grant. This support will enable us to continue...to coronavirus-related plasmids and to receive important coronavirus-related deposits. Our valiant lab ...We’re extremely proud of the work we’ve done to support COVID-19 research. Our collection of available ...operations for all ongoing processes are entirely self-supporting. We hire more staff as we need them to meet demand...approaching science funding organizations to request support for our operations until our normal work resumed...via Addgene. Perhaps if each of many grantors supporting research could provide Addgene with a small amount...

...SLC6A8 mutations in Creatine Transporter Deficiency Creatine Transporter Deficiency (CTD) is a rare X-... to be transported from the blood and cross the blood brain barrier using creatine transporters. However...However, mutations in the transporter SLC6A8 impede this transport and result in global creatine deficiency... the SLC6A8 gene, also known as the creatine transporter. The Association for Creatine Deficiencies (ACD...parent-let non-profit founded with the mission to support and drive research into CCDS. The brain and muscles... and induce their effector functions. It’s an important process that we need to understand so that we ...source of IL-36 cytokines in the skin making it important to study what is happening within the keratinocytes...

...fluorescent executioner caspase reporters. Several apoptosis reporters have been developed, such as the...transfer (FRET)-based executioner caspase reporters. In these reporters, the FRET pair is linked by a caspase...FlipCherry: Red fluorescence protease reporter To add to the fluorogenic reporter toolkit, the Shu Lab also designed...novel fluorogenic reporter that has a large dynamic range and high brightness. This reporter achieves approximately...Elmore, 2007). This reporter will allow scientists to observe both the spatial and temporal dynamics of apoptosis...fluorescence emission. FRET and fluorescence based reporters however have several limitations. First, FRET ...study apoptosis in vivo scientists need a protease reporter that has a large dynamic range and high brightness...

...issues. Improving customer support with self service We strive to make our support team available whenever...reliable Zendesk system that powers our email support, this portal houses answers to all of your most commonly...core values here is to provide unmatched customer support, as this is vital to ensuring that our services..., quickly, and this is why we have a dedicated support team that can help you through any search, ordering...an answer. Luckily, there’s a trend in customer support to address this exact problem, and it’s called ... to the repertoire of tools you can use to get support for our resources with our new Help Center! Created...the Help Center to make sure that, whatever your support needs are, we’ve got you covered! In addition to...

...What are Acoustic Reporter Genes (ARGs)? Fluorescent and bioluminescent reporter genes are widely used... to study gene expression in cells. But these reporters have limited use in vivo because they are based... penetration depth as well as high spatial and temporal resolution, making it ideal for visualizing gene...expression in vivo.  But what ultrasound lacked was reporter genes that could produce ultrasound contrast. ...our lab - the Shapiro Lab - developed Acoustic Reporter Genes (ARGs), which are sets of genes that, when...5202–5209 (2017). Bourdeau, R. W. et al. Acoustic reporter genes for noninvasive imaging of microorganisms... Hurt, R. C. et al. Genomically mined acoustic reporter genes for real-time in vivo monitoring of tumors...

...real time, but each of these reporters has its drawbacks. Acoustic reporter genes (ARGs) overcome these...existing bacterial reporters Limited Depth of Signal/Imaging: Fluorescent reporters are not as well suited...fluorescent or bioluminescent reporters usually only detects the signal of the reporter and fail to pinpoint what...signal is coming from.   Acoustic reporter genes (ARGs) Acoustic Reporter Genes (ARGs) are engineered gene...imaging tools The luciferase reporter gene The green fluorescent protein reporter gene Resources on Addgene.org... researchers have developed in vivo bacterial reporters that use fluorophores and luciferases to track...these limitations by using gas vesicle reporters that are detectable by an inexpensive and widely available...

...these plasmids. This is because in 2024 certain export control exceptions were made by the US Bureau of...majority of the countries we ship to, first on a temporary and now on a more lasting basis.   Table 1: Popular...   For researchers in countries with applicable export control exceptions for rabies and VSV-G genes, ... and we strive to keep our information updated, export controls and regulations are complex and may change...

... Figure 4: Reporters in Gene On include a mScarlet reporter, luciferase reporter, and neomycin resistance... editing. eGFP reporters for single base editing by APOBEC-Cas9 Though the ACE reporter quantifies APOBEC-Cas9...DSB in their previous reporter, the Harris Lab created a panel of eGFP reporters to quantify the on-target...editing reporters. Image from St. Martin et al., 2019. The Harris lab used these eGFP reporters to analyze...Transient reporter for editing enrichment (TREE) The Wang and Brafman lab developed a transient reporter for...cells segregated solely by a reporter of transfection, which only report the efficiency of plasmid delivery... functional reporter system to identify and enrich for base-editing activity The reporters mentioned above...

...Genome Institute of Singapore. Chapter 3: Biology research and resources in Singapore. Chapter 4: Life as...Chew, a researcher at the Genome Institute of Singapore who recently started his own lab. We discuss some...Principal Investigator at the Genome Institute of Singapore. His lab focuses on producing biotechnologies ... singapore...

...Problems with FRET kinase reporters Difficult to Multiplex: FRET reporters are comprised of two fluorescent...phosphatase-accessible. Kinase Translocation Reporters (KTRs) Kinase Translocation Reporters (KTRs) are genetically encoded...between the nucleus and the cytoplasm, nuclear export and import rates can influence KTR translocation. Work...least one site. Phosphorylation is particularly important for regulating signal transduction and measuring...single-cell kinase activity is FRET, but FRET reporters are challenging to design and difficult to multiplex...alternative tool with their Kinase Translocation Reporters (KTRs) whose cellular localization serves as a...multiplexed together. Challenging  to Design: FRET reporter activity depends on both the distance and the ...

...Orientation)) is a powerful tool that provides precise temporal and spatial control of gene expression in vivo...gene (e.g., channelrhodopsin-2, ChR2) fused to a reporter gene (e.g., mCherry) to easily detect opsin-expressing...- or reward-related behaviors. The high spatio-temporal precision and reversible modulation of FLEx vectors...recombinase in mouse primary afferents." Scientific reports 8.1 (2018): 7321. PubMed PMID: 29743652. PubMed...

...collection of reporter plasmids is amenable to Golden Gate assembly for turning the single-input reporters into...Neuron. 2019. PubMed PMID: 30704911. Acoustic reporter genes for noninvasive imaging of microbes in mammalian...contributed by Lukas Morgan  Listen to the acoustic reporter gene podcast segment In recent years, scientists...heavily on fluorescent protein genes. These optical reporters have limited deep-tissue performance, and sometimes...Mikhail Shapiro’s lab has developed four acoustic reporter gene (ARG) plasmids to better monitor gene expression...with a resolution of less than 100 µm, the ARG reporters could be successfully visualized in vivo using...demonstrates engineered gas vesicles as the first reporter genes using ultrasound and provide a major benefit...

...exist for all Addgene plasmids! In an effort to support standardized persistent identification of research...Research Resource identifiers (RRIDs) which are supported by SciCrunch. Other well known PID formats are... the journal’s specific author guidelines for reporting your materials. If you deposit a plasmid with ...plasmid URLs act as unique PIDs, we’re happy to support the RRID and compact identifier formats. Acknowledgments...

...remove a stop cassette upstream of an effector/reporter gene. In cells where the stop cassette is removed...https://doi.org/10.1101/788679 A destabilized GFP reporter for calcineurin activity in yeast Article contributed... it is an attractive drug target. Calcineurin reporters allow scientists to measure calcineurin dynamics... target calcineurin. Current calcineurin gene reporters are based on the bacterial βgal assay, and are...Büttner’s lab designed a calcineurin transcriptional reporter consisting of a quickly-degraded destabilized ...panels) equipped with destabilized GFP calcineurin reporter (blue) and stained with PI (pink) to indicate ...Image from Diessl et al., 2020. Find the GFP reporter calcineurin activity here Diessl et al., Microbial...

...cyclic AMP) Signaling reporter island (SiRI) with GFP-based fluorescent reporter cAMPr Spatial Multiplexing...maturation reporter (pmRFP-LC3) Dissection of the autophagosome maturation process by a novel reporter protein... DsRed1-E5 as a reporter for mitochondrial oxidative stress A novel MitoTimer reporter gene for mitochondrial...Multicoloured voltage reporters (QuasAr2) Bright and fast multicoloured voltage reporters via electrochromic...Neurotransmitters Cell Physiology Browse All Other Reporters Additional Resources Fluorescent biosensors are...biomolecule while largely preserving spatial and temporal cellular processes. Note that many of the articles...genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes A turquoise...

... 64034 pGL4Luc-RLuc Firefly, Renilla Dual reporter reporter vector allowing the study of biscistronic ...Multiple Multi-luciferase reporter vector including five transcriptional reporters for NF-kb, TGF-b, c-Myc...for gene expression assays and bioluminescent reporters. Plasmid...Collection Empty Backbones Expression Constructs Reporter Constructs Other Highlights Additional Resources...-lives, making them useful as transcriptional reporters, but are less-than-ideal protein tags due to their...or gene of interest into to create a luciferase reporter. Unless otherwise noted, these plasmids are meant...luciferase and renilla_polyA Firefly, Renilla Dual reporter vector. Insertion of 5' promoter/enhancer regions...

...tRNA is incorporated to the growing peptide chain. To expand the genetic code and incorporate a ncAA into...by reassigning specific codons to enable the incorporation of amino acids beyond the 20 standard ones, ... cell, the ncAA provided in the media can be incorporated using the existing protein translation machinery... tRNA and its cognate aaRS enzyme evolved to incorporate ncAAs. This plasmid often carries multiple tRNA...target plasmids in genome code expansion for the incorporation of non-canonical amino acids (ncAA) in a protein... are true. Always express first with a control reporter gene: GFP for E. coli or mCherry-GFP for mammalian...questions there. This peer-to-peer GCE community support service was set up in 2022 by the NIH-funded GCE4All...

...Optogenetics for E. coli Return to top Reporter Plasmids Reporter plasmids can be used to detect events...proteins and specific DNA sequences. ID Plasmid Reports Reporter Mechanism Expression Species PI 14474 pRU1161...protein interactions, controlled gene expression, reporter plasmids and genome engineering of bacteria. Plasmid...Visualization Protein Interactions Controlled Expression Reporter Plasmids Genome Engineering You may also like…...bacterium. Plasmids containing easily measurable reporter genes (e.g., LacZ, luciferase, or fluorescent ...transcription factor to bind to a small molecule, the reporter plasmid can be used to detect the presence of ...it. Bacterial one-hybrid (B1H) systems also use reporter genes to identify interactions between proteins...