We narrowed to 82 results for: /1000
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TypeBlog Post... Commercial Plasmid Prep Kit Amount 1000 Lanes 1000 Lanes 1000 Lanes Cost $200-$500 <$10 $20-30 ...convenient, commercial ladders cost between $200-$500 for 1000 lanes worth of ladder. The Penn State ladders are...plasmid prep protocol, it cost less than $10 to make 1000 lanes worth of the 100 bp or the 1 kb ladder. Using...
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Celebrate National Book Month with Addgene
TypeBlog Post...larger epic of fantasy novels. Set in a world where 1000 years ago the hero lost and the Dark Lord rules ...Napoleonic Wars and against something even more sinister. 1000 pages long. Incredibly imaginative, funny, bittersweet... -
Lentiviral Prep Service
TypeCollection...genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls. This backbone does not contain...unique sgRNAs targeting 19,114 human genes along with 1000 non-targeting controls Puromycin Doench , Root 73179...genes and containing 76,441 unique sgRNAs along with 1000 non-targeting controls. This backbone contains SpCas9...unique sgRNAs targeting 19,114 human genes along with 1000 non-targeting controls Puromycin Doench , Root Human... unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controls. This backbone does not contain...unique sgRNAs targeting 19,674 mouse genes along with 1000 non-targeting controls Puromycin Doench , Root Cas9... -
The Blue Flame Award: Celebrating Addgene's Most Requested Depositors
TypeBlog Post... but a select few are particularly special. Over 1000 of our 60,000+ deposited plasmids have been requested... -
The Power Behind NGS Plasmid Validation: seqWell
TypeBlog Post...barcodes. Rather than continuing to process 100s to 1000s of samples individually, plexWell™ reduces every... -
Antibodies 101: ChIP
TypeBlog Post...pieces of roughly equal size, typically between 200-1000 bp. This is to ensure that your downstream analysis... -
The time and cost required to make a plasmid
TypeBlog Post...and very cool 8,768,560 hours – or almost exactly 1000 calendar years! *Dammit, man, I’m a scientist... -
Visualizing Protein Turnover In Situ
TypeBlog Post...proximity ligation." Nature methods 3.12 (2006): 995-1000. PubMed PMID: 17072308. 4. Dieck, Tom, et al. "... -
Tips for Titering Your Lentiviral Preps
TypeBlog Post...particles. Studies have found that values can be 10-1000 fold higher than for functional assays depending... -
Uncovering Ancestral Bias in CRISPR Research
TypeBlog Post...Beroukhim and Boehm labs pulled data from more than 1000 CRISPR screens in DepMap to see if they could identify... -
Which Fluorescent Protein Should I Use?
TypeBlog Post...coefficient and quantum yield of the protein, divided by 1000. In many cases the brightness is compared to that... -
Droplet Digital PCR for AAV Quantitation
TypeBlog Post...used to calculate the viral titer: GC/mL = {[(R*C)(1000/V)]*D} R = Reaction volume C = Copies/uL V = Volume... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog Post... (response elements). Promoters are about 100 to 1000 base pairs long and found upstream of their target... -
CRISPR 101: Homology Directed Repair
TypeBlog Post...of template should have homology arms between 500-1000 bps. HDR efficiency with circular plasmid templates... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog Post...includes SpCas9, and shortern orthologs approximately 1000 amino acids in size. From the pool of shorter orthologs... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog Post...design 5 guide RNAs (gRNAs) targeting each of the 1000 genes you’d like to investigate in your next CRISPR... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog Post...Low 572/ 610 FP595 Tetramer Green Dark/ Red 70 - 1000 Medium 590/ 600 Choosing a photoactivatable... -
CRISPR 101: Multiplex Expression of gRNAs
TypeBlog Post... E. coli.” ACS Synthetic Biology 4(9) (2015):987-1000. PubMed PMID: 25822415. Find plasmids from this... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post...fibroblasts to myoblasts. Cell, 1987. 51(6): p. 987-1000. PubMed PMID: 3690668. 40. Ferber, S., et al., Pancreatic... -
Fluorescent Protein Guide: Empty Backbones
TypeCollection...exctinction coefficient and quantum yield divided by 1000. pKa is the pH at which fluorescence intensity is...