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We narrowed to 42 results for: VSV-G plasmid

Showing: 1 - 20 of 42 results

  1. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    ...reason, the top requested plasmid of 2017 is the lentivirus envelope plasmid pMD2.G from Didier Trono’s lab...and VSV-G. This plasmid has been used in studies of the secretome, ubiquitin ligase, cellular senescence...example (the top requested plasmids from 2015 and 2016 were CRISPR plasmids), but so are lentiviral vectors...lab! Lentivirus envelope vector pMD2.G This plasmid has been requested nearly 6,000 times (over 800 orders...orders in 2017) and expresses the VSV-G envelope protein under the control of the powerful mammalian CMV...determine the types of cells that it can infect. The VSV-G envelope is widely used and requested because it... types of cells. This means researchers who use VSV-G can deliver their genes of interest to a variety...

  2. Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes

    Type
    Blog Post
    ...Weinberg pMD2.G VSV-G envelope expressing plasmid Didier Trono pVSV-G Expresses VSV-G envelope protein...: Popular Rabies and VSV-G Plasmids Name Purpose Depositing Lab pCMV-VSV-G (Empty Backbone) Envelope...export control exceptions for rabies and VSV-G genes, plasmids ship out approximately three weeks faster...information on shipping plasmids to your country. Curious about how rabies plasmids are used in the lab? ...If you're an international researcher who uses plasmids containing genes from rabies and vesicular stomatitis...may have noticed it’s been easier to order these plasmids. This is because in 2024 certain export control...controls prescribed by the Australia Group, for these plasmids for the majority of the countries we ship to, ...

  3. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...protein for pseudotyping is VSV-G, or vesicular stomatitis virus G protein. VSV-G is a trimeric protein that...three plasmids: (1) The transfer plasmid, (2) the envelope plasmid, and (3) the packaging plasmid. These...one of the reasons VSV-G is used to transduce stem cells and neurons. However, VSV-G’s broad tropism can... on the envelope plasmid. Image from Crawford et al., 2020.   Broad tropism: VSV-G pseudotyping A commonly...cytotoxicity can vary. For example, the popular VSV-G envelope protein causes cells that it infects to...-based killing by anti-alphaGal antibodies. The VSV-G envelope protein is inactivated by serum regardless...surface to endocytose into the cell. The reason VSV-G is so commonly used is that it is good at infecting...

  4. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...available plasmid in the top 15. Find pSpCas9(BB)-2A-Puro (PX459) V2.0. pCMV-VSV-G - pCMV-VSV-G was deposited...expressing plasmid, such as our second most requested plasmid of all time, pMD2.G. Find psPAX2. pMD2.G - Also...from the Didier Trono lab is pMD2.G, a VSV-G envelope expressing plasmid. Envelope proteins determine which...of applications. This plasmid was the top requested plasmid of 2017. Find pMD2.G. pSpCas9(BB)-2A-GFP (... published in 2003, this plasmid predates Addgene’s founding. Find pCMV-VSV-G. pRSV-Rev - This 3rd generation...envelope expressing plasmid pMD2.G. Find pMDLg/pRPE. scramble shRNA - This plasmid from David Sabatini... top requested plasmid from 2016. You may notice the “V2.0” at the end of this plasmid which indicates...

  5. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    ...reason, the top requested plasmid of 2017 is the lentivirus envelope plasmid pMD2.G from Didier Trono’s lab...and VSV-G. This plasmid has been used in studies of the secretome, ubiquitin ligase, cellular senescence...example (the top requested plasmids from 2015 and 2016 were CRISPR plasmids), but so are lentiviral vectors...lab! Lentivirus envelope vector pMD2.G This plasmid has been requested nearly 6,000 times (over 800 orders...orders in 2017) and expresses the VSV-G envelope protein under the control of the powerful mammalian CMV...determine the types of cells that it can infect. The VSV-G envelope is widely used and requested because it... types of cells. This means researchers who use VSV-G can deliver their genes of interest to a variety...

  6. Export Control Regulations Lifted for Plasmids Containing Rabies and VSV-G Genes

    Type
    Blog Post
    ...Weinberg pMD2.G VSV-G envelope expressing plasmid Didier Trono pVSV-G Expresses VSV-G envelope protein...: Popular Rabies and VSV-G Plasmids Name Purpose Depositing Lab pCMV-VSV-G (Empty Backbone) Envelope...export control exceptions for rabies and VSV-G genes, plasmids ship out approximately three weeks faster...information on shipping plasmids to your country. Curious about how rabies plasmids are used in the lab? ...If you're an international researcher who uses plasmids containing genes from rabies and vesicular stomatitis...may have noticed it’s been easier to order these plasmids. This is because in 2024 certain export control...controls prescribed by the Australia Group, for these plasmids for the majority of the countries we ship to, ...

  7. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...protein for pseudotyping is VSV-G, or vesicular stomatitis virus G protein. VSV-G is a trimeric protein that...three plasmids: (1) The transfer plasmid, (2) the envelope plasmid, and (3) the packaging plasmid. These...one of the reasons VSV-G is used to transduce stem cells and neurons. However, VSV-G’s broad tropism can... on the envelope plasmid. Image from Crawford et al., 2020.   Broad tropism: VSV-G pseudotyping A commonly...cytotoxicity can vary. For example, the popular VSV-G envelope protein causes cells that it infects to...-based killing by anti-alphaGal antibodies. The VSV-G envelope protein is inactivated by serum regardless...surface to endocytose into the cell. The reason VSV-G is so commonly used is that it is good at infecting...

  8. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...available plasmid in the top 15. Find pSpCas9(BB)-2A-Puro (PX459) V2.0. pCMV-VSV-G - pCMV-VSV-G was deposited...expressing plasmid, such as our second most requested plasmid of all time, pMD2.G. Find psPAX2. pMD2.G - Also...from the Didier Trono lab is pMD2.G, a VSV-G envelope expressing plasmid. Envelope proteins determine which...of applications. This plasmid was the top requested plasmid of 2017. Find pMD2.G. pSpCas9(BB)-2A-GFP (... published in 2003, this plasmid predates Addgene’s founding. Find pCMV-VSV-G. pRSV-Rev - This 3rd generation...envelope expressing plasmid pMD2.G. Find pMDLg/pRPE. scramble shRNA - This plasmid from David Sabatini... top requested plasmid from 2016. You may notice the “V2.0” at the end of this plasmid which indicates...

  9. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...γ-retroviral packaging plasmids are not interchangeable. General envelope plasmids, such as VSV-G (Vesicular Stomatitis...glossary Plasmid Type Element Purpose Envelope VSV-G Vesicular stomatitis virus G glycoprotein...in trans by specific plasmids. This method, therefore, reduces the number of plasmids that are required ...encoded by the transfer plasmid, but instead are provided in trans by other plasmids or the packaging cell...do many γ-retroviral plasmids have similar names? The nomenclature of the plasmid backbone is meant to...pseudotyped to alter infectivity): The use of the VSV-G envelope provides the widest tropism or range of...components are needed: γ-Retroviral transfer plasmid encoding a transgene, sgRNA, or shRNA of interest...

  10. Retrovirus Plasmids

    Type
    Collection
    ...pCMV-VSV-G Envelope Envelope protein; use with packaging plasmid Weinberg 12259 pMD2.G Envelope VSV-G-expressing...envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG Envelope VSV-G-expressing envelope plasmid Nienhuis... and Packaging Plasmids Common γ-retroviral envelope and packaging plasmids. ID Plasmid Type Description...-expressing envelope plasmid Trono 1733 pCI-VSVG Envelope VSV-G recommended for the FELIX (FIV-based) ...Nienhuis and Salmon 22501 pHEF-VSVG Envelope VSV-G-expressing envelope plasmid Kasparov Popular γ-Retroviral...γ-Retroviral Transfer Plasmids Common γ-retroviral transfer plasmids. Want a retroviral plasmid with your gene ...retroviral plasmids at Addgene, including popular packaging, envelope, and transfer plasmids. Viral...

  11. Lentivirus Plasmids

    Type
    Collection
    ...transfer plasmids Trono 12259 pMD2.G N/A Envelope VSV-G-expressing envelope plasmid Trono 8454 pCMV-VSV-G N...envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG N/A Envelope VSV-G-expressing envelope plasmid Nienhuis... Viral Plasmids Lentiviral Plasmids Lentiviral Plasmids Additional Resources... Pooled Libraries All Viral Plasmids Packaging Plasmids Transfer Plasmids Lentiviruses, a subset of retroviruses...among multiple plasmids. Some plasmids (typically called packaging and envelope plasmids) encode components...and 3rd generation transfer plasmids Weinberg 1733 pCI-VSVG N/A Envelope VSV-G recommended for the FELIX...titer Kasparov 22501 pHEF-VSVG N/A Envelope VSV-G-expressing envelope plasmid Kasparov 22500 pNHP 2nd Packaging...

  12. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...γ-retroviral packaging plasmids are not interchangeable. General envelope plasmids, such as VSV-G (Vesicular Stomatitis...glossary Plasmid Type Element Purpose Envelope VSV-G Vesicular stomatitis virus G glycoprotein...in trans by specific plasmids. This method, therefore, reduces the number of plasmids that are required ...encoded by the transfer plasmid, but instead are provided in trans by other plasmids or the packaging cell...do many γ-retroviral plasmids have similar names? The nomenclature of the plasmid backbone is meant to...pseudotyped to alter infectivity): The use of the VSV-G envelope provides the widest tropism or range of...components are needed: γ-Retroviral transfer plasmid encoding a transgene, sgRNA, or shRNA of interest...

  13. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...envelope plasmids are two of Addgene’s most requested plasmids! Lentiviral particles with the VSV-G envelope...consisted of three plasmids: the packaging, envelope, and transfer plasmids. The packaging plasmid carried a mutated...Hoyah G, Winemiller A, Andre K, Stein D, Blick G, Greenberg RN, Kinder C, Zolopa A, Binder-Scholl G, Tebas...Many CRISPR plasmids are designed for lentiviral use, including Addgene’s top requested plasmid in 2015: ...different viral envelope protein (Env), usually from VSV-G due to wide infectivity, is often used as a substitute...itself due to a few missing proteins. The envelope plasmid contained a viral envelope to dictate the tropism...the vector could infect. Finally, the transfer plasmid encoded the desired transgene flanked by HIV-1 ...

  14. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...envelope plasmids are two of Addgene’s most requested plasmids! Lentiviral particles with the VSV-G envelope...consisted of three plasmids: the packaging, envelope, and transfer plasmids. The packaging plasmid carried a mutated...Hoyah G, Winemiller A, Andre K, Stein D, Blick G, Greenberg RN, Kinder C, Zolopa A, Binder-Scholl G, Tebas...Many CRISPR plasmids are designed for lentiviral use, including Addgene’s top requested plasmid in 2015: ...different viral envelope protein (Env), usually from VSV-G due to wide infectivity, is often used as a substitute...itself due to a few missing proteins. The envelope plasmid contained a viral envelope to dictate the tropism...the vector could infect. Finally, the transfer plasmid encoded the desired transgene flanked by HIV-1 ...

  16. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    ...these plasmids from Addgene. This publication then resulted in the development of a new plasmid, pCMV14...continue having access to plasmids during the COVID-19 pandemic. Over 7,000 plasmids for COVID-19 research...work to share plasmids to all scientists Resources on Addgene.org Find COVID-19 plasmids at Addgene Browse...the lentiviral production and packaging vectors VSV-G and psPAX2, which were used in a publication by ...Biological repositories that distribute mouse strains, plasmids, cell lines, and much more have been critical ...science is truly a global endeavor. Case study 2: Plasmids Another example of reagents that have broad applications...requested from Addgene. Figure 2: Total COVID-19 plasmids requested from Addgene from March 23, 2020 to ...

  17. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...with most lentiviral vectors being packaged using VSV-G. In contrast, AAV can be packaged in a variety of... The most obvious workaround is to use a multi-plasmid system, where you have one AAV vector dedicated...E., Kuhn, M., Farouni, R., Lagler, S., Kalamakis, G., Lischetti, U., Jabaudon, D., & Platt, R. J. (2023...Gessler, D. J., Zhan, W., Gallagher, T. L., & Gao, G. (2024). Adeno-associated virus as a delivery vector...

  18. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...gamma-retroviral packaging plasmids are not interchangeable. General envelope plasmids, such as VSV-G, may be used ...separate envelope and packaging plasmids as well as transfer plasmids. This concept ensures safe handling...Learn more about CRISPR plasmids for transcriptional inhibition. Retroviral Plasmid Kit Retroviral Barcoding...expressing new shRNA sequences pLKO (Plasmid #10878) This plasmid has been used by The RNAi Consortium... genes and transgene expression across several plasmids. The most commonly used viral vector systems are...popular packaging and gamma-retroviral envelope plasmids here. Lentiviral vectors are very versatile tools...guide. Popular packaging and lentiviral envelope plasmids are listed here. Please note that both lentiviruses...

  19. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...  The envelope plasmid typically encodes the vesicular stomatitis virus G protein (VSV-G), which facilitates...integrase-deficient packaging plasmid instead of the standard packaging plasmid. For more details on how to...lentiviral genome is distributed across multiple plasmids. For simplicity, we’ll use the second-generation...system as an example.  The lentiviral transfer plasmid encodes your insert of interest, flanked by long...this portion into the host genome. The packaging plasmid contains Gag, Pol, Rev, and Tat genes. Specifically...into the host cell.  Transfecting these three plasmids into cells enables the production of lentiviral... vector using an integrase-deficient packaging plasmid available at Addgene: psPAX2-D64V. This construct...
Showing: 1 - 20 of 42 results