We narrowed to 54 results for: aav gfp cre

...efficiency of AAV-PHP.B versus AAV-PHP.eB in the CNS. A GFP transgene was packaged into either an AAV-PHP.B or... comparison, capsids were packed with a GFP transgene and GFP intensity and transduction efficiency of...efficiency of AAV-PHP.eB and AAV-PHP.S versus their parental AAV vectors. What can these AAV’s be used for...efficiency of AAV.9 versus AAV.PHP.S in the PNS. A GFP transgene was packaged into either an AAV.9 or an AAV.PHP.S...stands for Cre Recombinase-based AAV Targeted Evolution. In this technique, a pool of mutant AAV capsid (...Search for AAV Vectors to use with your research. Visit Our AAV Guide Page for info on AAV components,... with cell-type-specific Cre, the polyA sequence is inverted only in the Cre-expressing tissues. This ...

...GBPs/GFP. The new system, CRE-DOG (Cre Dependent On GFP), is activated by GFP and derivatives GFP and ...to bind GFP. The idea of using GFP as a scaffold suddenly seemed very realistic. Envisioning GFP as a substrate...Connie Cepko Combining Cre and GFP with Cre-DOGs The T-DDOG results showed that GFP could regulate transcription...induced in ~76% of GFP+ cells, and 100% of DsRed+ cells also expressed GFP. Using AAV constructs, Tang ...At Addgene, we love GFP, and we’re always excited when depositors find new ways to make this workhorse...transcription or Cre recombinase activity only in the presence of GFP. These systems, known as T-DDOG and Cre-DOG,...lines expressing GFP in specific cell types to do more than just label cells. If GFP could be co-opted...

... how Cre-lox recombination works. Advances in Cre-lox recombination have included inducible Cre, such...transgenes and Cre, Cai et al. also created an Autobow construct containing both Cre and XFPs. Cre production...adeno-associated viral vectors (AAV) available - AAV-EF1a-BbChT and AAV-EF1a-BbTagBY. These constructs ...continued utility of Cre-lox. Check out our previous blog post, Plasmids 101: Cre-lox, if you need a quick...membrane tethered cyan). Without Cre recombination, RFP is expressed. Cre can mediate one of two deletions...construct, OFP (orange), is expressed when Cre is absent; stochastic Cre excision results in one of three recombinations...system employs Cre-mediated deletion, the design of Brainbow-2.1 cleverly combines Cre-mediated deletion...

...channelrhodopsin, fused to a fluorescent GFP marker (CatCH-GFP). The lab deposited AAV plasmids containing the active... of Cre-mCherry and a second promoter to drive expression of a Cre-dependent fluorescent CatCH-GFP reporter...Expression of GFP will only result in cells where Cre-mCherry is also expressed. If Cre-mCherry expresses...horizontal cells transduced by both AAVs would express Cre-mCherry and GFP.   Figure 2: Synthetic promoters... photoreceptors and horizontal cells, and Cre-dependent-GFP expresses in horizontal cells and ganglion...Adeno-associated virus (AAV) is a popular tool for gene delivery, but it has a drawback: how do you ensure...Performance of synthetic promoter AAVs in mice All of these synthetic promoter AAVs were first injected into the...

...makes an AAV transfer plasmid an AAV transfer plasmid. ITR sequences are 145 bases each and AAV plasmids... want to do, but it requires some AAV. You’ve never worked with AAV before, but you aren’t going to let...experiments, making AAV starts with some plasmids. You just need three plasmids to start making AAV:  the packaging... the AAV transfer plasmid and take a look at each of its parts. Figure 1: Parts of an AAV transfer...   Key elements of an AAV plasmid First let’s talk about the key parts of an AAV plasmid. Inverted terminal...packaged into the AAV molecule. What’s outside of the ITRs does not get packaged in the AAV vector. The cloning...ITR loss abolishing AAV packaging. To prevent ITR loss, it’s best to propagate AAV constructs in recombination-deficient...

...consider our AAV Packaged on Request service for more options. Addgene blog Plasmids 101: Cre-lox Cre-ating ...Controlling Protein Activity with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and FLEx Vector Expression...Plasmid Collections Recombinases Cre-Lox and Other Site-Specific Recombinases Cre Plasmids Flp Plasmids Dre ...prevent gene expression in the absence of Cre. In the presence of Cre, the stop codon is excised, and gene ...until Cre is present, at which time the gene will be disrupted or deleted. shRNA Expression: Cre-lox can...interest, Genes A and B. When Cre is absent, only Gene A is translated correctly. Cre expression excises Gene...double-crossover event. Toxicity Cre-lox recombination is a very useful tool, but Cre expression can be toxic ...

...EF1a Nuc-flox(mCherry)-EGFP Brandon Harvey 50457 AAV2 pAAV-hSyn-DIO-EGFP Bryan Roth 50459 AAV1 pAAV-hSyn-DIO-mCherry..., AAV Retrograde, AAV2, AAV5, AAV8, and AAV9). Once activated, this DREADD acts through the Gq pathway...respectively of a GFP dependent Cre. The two segments come together in the presence of GFP to form a functional...gene activation to cells expressing both Cre and GFP. The GFP dependent FLPo, Flp-DOG (75469), is also...proteins ChR2 for targeted expression DREADDs GFP dependent Cre and FLPo And more!  New lentiviral preps...plasmids in new serotypes, as well as a new Cre dependent GFP control plasmid for expression in forebrain...Serotype Name 114471 AAV2 pAAV-Ef1a-fDIO mCherry 83900 AAVrg pAAV-mDlx-GFP-Fishell-1 Biosensors...

... 20 ul aliquots in AAV1, AAV2, AAV5, AAV8, AAV9, and AAV Retrograde. You can also request the specific...: pGP-AAV-syn-jGCaMP7f-WPRE (104488-AAV8) pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (104492-AAV8) pGP-AAV-syn-jGCaMP7c...Flp-dependent Cre AAV in AAV9 and AAVrg. See our new additions here: Cre vectors pAAV-EF1a-fDIO-Cre (121675...26976-AAV5) pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (112677-AAV5) Calcium Sensors pGP-AAV-syn-jGCaMP7b-WPRE.... Our new AAVs include: EGFP-expressing AAV for serotype testing Calcium sensors and GABA sensors Optogenetics...is now complete! pAAV-CAG-GFP (plasmid 37825) and pAAV-hSyn-EGFP (plasmid 50465) are now available as 20...pAAV-hSyn-DIO-EGFP (50457-AAV5): The Synapsin promoter directs broad, neuronal expression. AAV pCAG-FLEX-...

...prep pAAV-CAG-GFP AAV9-X1.1 Controls Edward Boyden New serotype pAAV-hSyn-EGFP AAV PHP.eB Controls...levels compared to AAV9. You can now target these cells with AAV-CAG-GFP packaged in AAV9-X1.1!  We also ...pAAV-EF1a-fDIO-Cre AAV5 Recombinases Esteban Engel New serotype pAAV-hSyn-Cre-P2A-dTomato AAV PHP.eB Recombinases... Serotype Category Depositor  Notes AAV-GfaABC1D-Cre-4x6T AAV5 Recombinases Stanley Thomas Carmichael... (Alias: CN3905) AAV PHP.eB Controls Jonathan Ting New viral prep pAAV-CaMKIIa-EGFP AAV1 Controls Bryan...H134R)-EYFP AAV2 Optogenetics Karl Deisseroth New serotype AAV-CamKIIa-jGCaMP8s-WPRE AAV5 Biosensors ...first Addgene prep packaged in the AAV9-X1.1 serotype.  In rodents, AAV9-X1.1 enables expression in brain...

...diversified) 37825  AAV8*, AAV9*  pAAV-CAG-GFP 50465  AAV8*, AAV9*  pAAV-hSyn-EGFP *Also coming in...pAAV-mDlx-NLS-mRuby2 CAG-NLS-GFP pAAV-hSyn-mCherry Read on to learn more!  Calcium sensor AAV The GFP-based calcium...pAAV-Syn-Archon1-KGC-GFP-ER2) and cre-dependent version (pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2]) of this membrane-embedding... 50363  AAV5  AAV phSyn1(S)-DreO-bGHpA 121675  AAV9  pAAV-EF1a-fDIO-Cre (FLP-dependent CRE) 55634...pAAV.hSyn-FLEX.iGABASnFR.F102G pAAV-Syn-Archon1-KGC-GFP-ER2 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] pAAV-FLEX-tdTomato pAAV-GFAP104...SYN1 HA-hM4D(Gi) 119741  AAV5  AAV SYN flex PSAM4 GlyR IRES EGFP Chimeric channels for neuronal manipulation...manipulation 119742  AAV5  AAV SYN PSAM4 GlyR IRES EGFP Chimeric channels for neuronal manipulation Recombinases...

...Schnutgen et al. Nat Biotech 2003): In the presence of Cre, GFP expression is lost, allowing for rescued expression...well as for improvement of optogenetic adeno-associated virus (AAV) vectors. Listed below are a few additional... should contain a reporter DNA sequence (such as GFP) in the sense orientation, followed by coding sequence...express GeneR only in the presence of Cre. In this example, Cre would first invert the reporter and GeneR...a cell or tissue-specific Cre is provided. Since the FLEx switch ensures Cre-dependent reporter expression...orientation in the presence of Cre. A similar strategy can be employed for "Cre-off" (i.e. your gene of interest...in the sense orientation and Cre is used to flip it to "off"). These Cre-off switches may be referred ...

...Cre-dependent GFP expression was detected with direct fluorescence. GFP was not detected in the absence of Cre. mCherry...[Jaws-KGC-GFP-ER2] (Addgene 84445-AAVrg) alone at 1.1E6 vg/mL, or both. Two days later, Cre-dependent ... and quality control are described below. AAV Production AAV distributed by Addgene has been produced ...purity of the AAV preparation. Figure 1: Silver staining of purified and non-purified AAV subjected...   Figure 2: AAV Pro cells were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone...prep # 55632-AAVrg). pAAV-CAG- FLEX-rc [Jaws-KGC-GFP-ER2] was a gift from Edward Boyden (Addgene viral...arrowhead). Scale bar = 100 nm.   AAV resources Browse AAV preps available from Addgene. After receiving...

...transduced at levels similar to that of a single AAV expressing GFP. The lab tested the base editors in vivo ...combines the Cre/lox system with optogenetics. Cre activity is controlled by red light by splitting Cre and fusing...here.        AAV delivery of split base editors Article contributed by Jennifer Tsang AAVs are a great ...voltage indicators, however, fall short compared to GFP-based sensors in terms of response and brightness...reduces toxicity as compared to previous Cre/lox or drug-induced Cre systems (ex: tamoxifen). As the name ... dimerize, bringing each terminus of Cre together to restore Cre activity. As soon as red light or PCB...proteins separate, inactivating Cre. This is critical for reducing Cre toxicity in long-term experiments...

...) pAAV-CAG-GFP 50465 AAV8, 9 (20 µL size serotype testing) AAV-hSyn-EGFP Biosensor AAV The ability...popular control AAV encoding GFP. Check out our inventory of serotype testing AAV New control AAVs Plasmid...AAV1 pAAV-CAG-GFP 59462  AAV2 pAAV-CAG-tdTomato (codon diversified) 114472  AAV5, AAV8 pAAV-hSyn-mCherry...Serotype Name 119741  AAV5  AAV SYN flex PSAM4 GlyR IRES EGFP Recombinase AAV Finally, we are excited to...Flp-expressing AAV, as well as a Cre-dependent Flp-expressing AAV. See our recombinase AAV inventory here...testing) pAAV-CAG-GFP 50465 AAV1, 2, 5, rg (20 µL size serotype testing) pAAV-hSyn-EGFP Recombinases...Name 99039  AAVrg pAAV-CamKII-ArchT-GFP (PV2527) 105679  AAV5 ppAAV-CKIIa-stGtACR1-FusionRed New...

...line expressing a GFP-tagged version of a protein of interest. The binding of the GFP nanobody to its target... proximity to the GFP-tagged protein (albeit slightly offset due to the nanobody-GFP interaction). The...labeling Increased neuronal expression using Cre-mediated AAV Targeted Evolution method (M-CREATE) dCas9...ligase TurboID fused to a conditionally stabilized GFP-nanobody rather than a specific protein of interest...-4.   Increased neuronal expression using Cre-mediated AAV Targeted Evolution method (M-CREATE) by: Brian...previously developed the multiplexed version of the Cre-mediated AAV Targeted Evolution method (M-CREATE), in which...generational evolution of the AAV capsids (left) and transduction patterns (right).  Both AAV-PHP.eB and the AAV....

... Mammalian shRNA Tools for RNAi! Cre-Lox technology CAG-GFP-IRES-CRE (Plasmid #48201) This retroviral ...retroviral vector from the Gage lab encodes GFP and Cre-recombinase. The Cre-lox system is a technology that can...gamma-retrovirus, lentivirus, Adenovirus, and Adeno-associated virus (AAV). If you are wondering which virus fits...Vectors   Expressing your gene of interest pMKO.1 GFP (Plasmid #10676) Retroviral vector for cloning and...and expressing your gene of interest with GFP expression.  Cloning and expressing RNAi pMKO.1 puro (Plasmid...of mouse transgenics. Experimental uses include: Cre-dependent gene expression, gene knockout, and selection... how to use the pLKO.1 vector can be found here. Cre-Lox technology Puro.Cre empty vector (Plasmid #17408...

...fused to GFP forms fluorescent biomolecular condensates. A GFP-nanobody fused to the killswitch (GFP-nb–KS...the EGFP, and the TEV protease. Once the recipient cells take up the Cre-tagged mitochondria, Cre is released...protein of interest or targeted to a GFP fusion protein using an anti-GFP nanobody (Figure 4). Once incorporated... excises the DsRed-STOP to trigger expression of EGFP. Figure 2: Schematic of the MitoTRACER system...Micropeptide killswitch for biomolecular condensates AAV-CRISPRa-engineered fat cells fight cancer Frugal ...interactions. Plus, the tools used here (also available as AAV Packaged on Request) will be useful for related studies...mitochondria (Hoover et al., 2025). MitoTRACER relies on a Cre-lox switch to turn recipient cells from red to green...

...PI 37825 AAV-CAG-GFP CAG GFP Control Edward Boyden 51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP, Cre-dependent... 50465 pAAV-hSyn-EGFP Syn EGFP Control Bryan Roth 50469 pAAV-CaMKIIa-EGFP CamKII EGFP Control Bryan Roth...pAAV-mDlx-GFP-Fishell-1 Dlx GFP Control Gordon Fishell 83895 pAAV-hDlx-Flex-GFP-Fishell_6 Dlx GFP Control...pAAV-hSyn-DIO-EGFP Syn EGFP, Cre-dependent Control Bryan Roth 55650 pAAV-hSyn Con/Fon EYFP Syn EYFP, Cre and Flp-dependent...pENN.AAV.EF1a.eGFP.WPRE.rBG EF1a EGFP Control James M. Wilson 116869 pAAV-CAG-H2B-GFP CAG GFP Control Loren Looger...24593 AAV-pgk-Cre PGK Cre expression Recombinases Patrick Aebischer 55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a...pAAV-hsyn-Jaws-KGC-GFP-ER2 Syn Inhibitor Optogenetics Edward Boyden 84445 pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] CAG...

...CAG-NLS-GFP CAG NLS-GFP Constitutive PHPeB Viviana Gradinaru 116869 pAAV-CAG-H2B-GFP CAG H2B-GFP Constitutive...pAAV-EF1a-mitoGFP-DIO EF1a mito-GFP Cre dependent 2 Maarten Kole 190155 AAV_pMBP-EGFP-caax MBP EGFP-caax Constitutive...mCherry Cre dependent 1, 2, 5, 8, 9, rg* Bryan Roth 51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP Cre dependent...pAAV-hSyn-EGFP hSyn EGFP Constitutive 1, 2, 5, 8, 9, 11, rg*, PHP.eB Bryan Roth 50469 pAAV-CaMKIIa-EGFP CaMKIIa...EF1a mScarlet Cre dependent 1, 5 Karl Deisseroth 50457 pAAV-hSyn-DIO-EGFP hSyn EGFP Cre dependent 1, 2...-CAG-FLEX-EGFP CAG EGFP Cre dependent 1 Ian Wickersham 104052 pAAV-CAG-DIO-EYFP CAG EYFP Cre dependent...Fluorophore/Tag Activity Serotype PI 37825 AAV-CAG-GFP CAG GFP Constitutive 1, 2, 5, 6, 8, 9, 11, rg*, PHPeB...

...optogenetic AAV for expression in forebrain GABA-ergic interneurons. These include mDlx-GFP, hDlx-FLEX-GFP, hDlx-Flex-dTomato...syn-FLEX-axon-jYCaMP1s. Find these AAVs at Addgene Cre-dependent EYFP AAV in the new serotype PHP.V1 that...introduced into the hPSC genome using AAV1 and contain an EGFP reporter. Expression of the dCas9-KRAB activator...vesicular brain cells. Find this AAV at Addgene New serotypes for the Cre-dependent Flpo recombinase pEF1a-DIO-FLPo-WPRE-hGHpA...pEF1a-DIO-FLPo-WPRE-hGHpA, which is now also available in AAV5 and AAVrg. Find these AAVs at Addgene New controls, biosensor...plasmids at Addgene! CRISPR RNP electroporation and AAV donor infection (CRISPR-READI) enables genome engineering...sensors include syn-jYCaMP1s (slow variant), the Cre-dependent syn-FLEX-jYCaMP1s, and the axon-targeted...