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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 48 results for: ef1a

Showing: 1 - 20 of 48 results

  1. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...AAVrg pAAV-Ef1a-DIO EYFP 114471 AAV5 pAAV-Ef1a-fDIO mCherry 112677 AAV2 pOTTC1032 - pAAV EF1a Nuc-flox...pAAV-Ef1a-fDIO mCherry 99130 AAVrg pAAV-mDlx-NLS-mRuby2 112677 AAV1, AAV2 pOTTC1032 - pAAV EF1a Nuc-flox...dependent. Plasmid Serotype Name 124603 AAV9 pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 124650 AAV9...Nuc-flox(mCherry)-EGFP 27056 AAVrg pAAV-Ef1a-DIO EYFP 50457 AAV2 pAAV-hSyn-DIO-EGFP   Biosensor ...GP-AAV-CAG-FLEX-jGCaMP7s-WPRE 105714 AAV8 pAAV-Ef1a-fDIO-GCaMP6s Neurotransmitter sensors: Dopamine... Plasmid Serotype Name 87306 AAVrg, AAV5 AAV pEF1a-DIO-FLPo-WPRE-hGHpA    Additional resources...

  2. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...Depositor 27056 AAVrg pAAV-Ef1a-DIO EYFP Karl Deisseroth 11447 AAV5 pAAV-Ef1a-fDIO mCherry Karl Deisseroth...Depositor 69570 AAV1 pAAV-EF1a-N-CretrcintG Connie Cepko 69571 AAV1 pAAV-EF1a-C-CreintG Connie Cepko ...Deisseroth 112677 AAV2 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP Brandon Harvey 50457 AAV2 pAAV-hSyn-DIO-EGFP... 75469 AAV1 pAAV-EF1a-Flp-DOG-NW Connie Cepko Viral vectors coming soon! These vectors listed below...Controls Plasmid Serotype Name 114471 AAV2 pAAV-Ef1a-fDIO mCherry 83900 AAVrg pAAV-mDlx-GFP-Fishell...pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-Puro: Expression of the SARS-CoV-2 N protein  pLVX-EF1alpha-SARS-CoV...the E protein (pLVX-EF1alpha-SARS-CoV-2-E-2xStrep-IRES-Puro) and NSP2 (pLVX-EF1alpha-SARS-CoV-2-nsp2-2xStrep-IRES-Puro...

  3. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (112677-AAV5 and 112677-AAVrg): The EF1a promoter directs...50363-AAV5) Flp vectors pAAV-EF1a-mCherry-IRES-Flpo (55634-AAV1) pAAV-EF1a-Flpo (55637-AAV1) Viral vectors...AAVrg. See our new additions here: Cre vectors pAAV-EF1a-fDIO-Cre (121675-AAV9, 121675-AAVrg)  pENN.AAV.hSyn.Cre.WPRE.hGH...pAAV-hSyn-hChR2(H134R)-mCherry (26976-AAV5) pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (112677-AAV5) Calcium Sensors...

  4. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...Serotype Name 55634  AAV1  pAAV-EF1a-mCherry-IRES-Flpo 55637  AAV1  pAAV-EF1a-Flpo Chemogenetics Plasmid...Serotype Name 55637  AAVrg pAAV-EF1a-Flpo 87306  AAV1 AAV pEF1a-DIO-FLPo-WPRE-hGHp Other new viral...-jGCaMP7c variant 1513-WPRE 105714  AAV8  pAAV-Ef1a-fDIO-GCaMP6s Optogenetics Plasmid Serotype...pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1 124603  AAV9  pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40  Additional...

  5. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ... Stanley Thomas Carmichael New viral prep pAAV-EF1a-fDIO-Cre AAV5 Recombinases Esteban Engel New serotype...pAAV-CaMKIIa-EGFP AAV1 Controls Bryan Roth New serotype pAAV-Ef1a-DIO mScarlet AAV5 Controls Karl Deisseroth New serotype...AAV1 Optogenetics Edward Boyden New serotype pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE AAV1 Optogenetics Karl...

  6. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ... 121675  AAV9  pAAV-EF1a-fDIO-Cre (FLP-dependent CRE) 55634  AAV1  pAAV-EF1a-mCherry-IRES-Flpo 55637... 55637  AAV1  pAAV-EF1a-Flpo Calcium sensors Plasmid Serotype Name 104496  AAV1  GP-AAV-CAG-FLEX-jGCaMP7f-WPRE...

  7. Viral Production at Addgene

    Type
    Blog Post
    ...: AAV Pro cells were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone at 1.7E6 viral...Cre. mCherry expression alone was detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from Karl Deisseroth...

  8. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...enhancer region. Can be silenced in some cell types. EF1a General expression mRNA Strong mammalian expression...factor promoter Constitutive  Analogous to mammalian EF1a promoter. GDS General expression mRNA  Strong ...

  9. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...pAAV-hSyn-GRAB_ACh3.0 AAV9 Biosensors Li New viral service pAAV-Ef1a-fDIO-ChrimsonR-tdTomato AAV9 Controls Jensen New...Yizhar New viral service with multiple serotypes pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE AAV1, AAV5 Optogenetics...

  10. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...Optogenetics Ofer Yizhar New viral tool pAAV-Ef1a-fDIO hChR2(H134R)-EYFP AAVrg Optogenetics Karl Deisseroth...

  11. New Viral Vectors - March 2024

    Type
    Blog Post
    ...AAV PHP.eB Controls Deisseroth New serotype pAAV-Ef1a-mCherry  AAV PHP.eB Controls Deisseroth New serotype...PHP.eB Controls Wilson New viral vector pENN.AAV.EF1a.eGFP.WPRE.rBG AAV PHP.eB Controls Wilson New serotype...

  15. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...AAV1 Biosensor Marianne Fyhn New viral prep pAAV-Ef1a-fDIO EYFP AAV5. AAV8, AAVrg Control Karl Deisseroth...

  17. Control AAV Preps

    Type
    Collection
    ...45185 AAV-EF1a-BbTagBY EF1a TagBFP and EYFP Cre dependent 9 Sanes 45186 AAV-EF1a-BbChT EF1a mCherry and...pAAV-Ef1a-Coff/Fon-BFP EF1a BFP Flp dependent 8 Deisseroth 137134 pAAV-Ef1a-Coff/Fon-mCherry EF1a mCherry...Wickersham 137130 pAAV-Ef1a-Con/Foff 2.0-BFP EF1a BFP Cre dependent 8 Deisseroth 137133 pAAV-Ef1a-Con/Foff 2.0-...-mCherry EF1a mCherry Cre dependent 8 Deisseroth 137137 pAAV-Ef1a-Con/Foff 2.0-oScarlet EF1a oScarlet ... pAAV-Ef1a-fDIO mCherry EF1a mCherry Flp dependent 1, 2, 5, 8, 9, rg* Deisseroth 55641 pAAV-Ef1a-fDIO ... EYFP EF1a EYFP Flp dependent 1, 2, 5, 8, 9, rg* Deisseroth 128434 pAAV-Ef1a-fDIO-tdTomato EF1a tdTomato...137129 pAAV-Ef1a-Con/Fon-BFP EF1a BFP Cre and Flp dependent 8 Deisseroth 137132 pAAV-Ef1a-Con/Fon-mCherry...

  18. Recombinases AAV Preps

    Type
    Collection
    ...121675 pAAV-EF1a-fDIO-Cre EF1a none 1, 5, 8, 9, rg* Engel , Nectow 69570 pAAV-EF1a-N-CretrcintG EF1a none 1...Serotype(s) PI 140135 pAAV-EF1a-iCreV EF1a none 1, PHPeB Zeng 140136 pAAV-EF1a-iDreV EF1a none 1, PHPeB Zeng ...55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a mCherry (not a fusion tag) 8, rg* Deisseroth 55636 pAAV-EF1a-Cre EF1a...55634 pAAV-EF1a-mCherry-IRES-Flpo EF1a mCherry (not a fusion tag) 1, rg* Deisseroth 55637 pAAV-EF1a-Flpo EF1a... EF1a none 1, 2, rg* Deisseroth 87306 AAV pEF1a-DIO-FLPo-WPRE-hGHpA EF1a none 1, 2, 5, 8, 9, rg* Zhang... 1 Cepko 69571 pAAV-EF1a-C-CreintG EF1a none 1 Cepko 105550 pAAV.GFAP.Cre.WPRE.hGH GFAP none 5, PHPeB ...Zhang 75469 pAAV-EF1a-Flp-DOG-NW EF1a none 1 Cepko 183412 pAAV-CAG-FlpO CAG none 1, rg* Janelia Light-Inducible...

  19. Retrograde AAV viral preps

    Type
    Collection
    ...27056 pAAV-Ef1a-DIO EYFP EF1a EYFP, Cre-dependent Control Deisseroth 55641 pAAV-Ef1a-fDIO EYFP EF1a EYFP, ...114470 pAAV-Ef1a-mCherry EF1a mCherry Control Deisseroth 114471 pAAV-Ef1a-fDIO mCherry EF1a mCherry, Flp-dependent... 55636 pAAV-EF1a-Cre EF1a Cre expression Recombinases Deisseroth 55637 pAAV-EF1a-Flpo EF1a Flpo expression...176756 AAV-EF1a-jGCaMP8f-WPRE Ef1a GCaMP8f Calcium sensor Looger 176757 AAV-EF1a-jGCaMP8m-WPRE EF1a GCaMP8m...-hGHpA EF1a Cre-dependent Flpo recombinase Recombinases Zhang 121675 pAAV-EF1a-fDIO-Cre EF1a Flp-dependent...176758 AAV-EF1a-jGCaMP8s-WPRE EF1a GCaMP8s Calcium sensor Looger 179254 AAV-CAG-jGCaMP8f-WPRE EF1a GCaMP8f...Deisseroth 55638 pAAV-EF1a-vCre EF1a VCre expression Recombinases Deisseroth 87306 AAV pEF1a-DIO-FLPo-WPRE-hGHpA...

  20. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Items 55636 pAAV-EF1a-Cre None Yes 55632 pAAV-Ef1a-mCherry-IRES-Cre None Yes 55637 pAAV-EF1a-Flpo None Yes... 55634 pAAV-EF1a-mCherry-IRES-Flpo None Yes 55638 pAAV-EF1a-vCre None Yes 55635 pAAV-EF1a-sCre None No...55641 pAAV-Ef1a-fDIO EYFP Flp Yes 55640 pAAV-Ef1a-dDIO hChR2(H134R)-EYFP Dre No 55639 pAAV-Ef1a-fDIO hChR2...pAAV-Ef1a-Coff/Fon-mCherry Flp AND NOT Cre Yes 137135 pAAV-Ef1a-oScarlet None Yes 137136 pAAV-Ef1a-Con/...137125 pAAV-Ef1a-sRGECO None Yes 137126 pAAV-Ef1a-Con/Fon-sRGECO Cre AND Flp Yes 137127 pAAV-Ef1a-Con/Foff...EYFP Flp Yes 126080 pAAV-Ef1a-sCreDIO hChR2(H134R)-eYFP Scre No 126081 pAAV-Ef1a-vCreDIO hChR2(H134R)-eYFP...NOT Cre No 231927 pAAV-Ef1a-Coff/Fon-EYFP Flp AND NOT Cre Yes 137129 pAAV-Ef1a-Con/Fon-BFP Cre AND Flp...
Showing: 1 - 20 of 48 results