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We narrowed to 34 results for: puromycin

Showing: 1 - 20 of 34 results

  1. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    ...SpCas9 is fused to the puromycin resistance gene through a 2A peptide. The puromycin resistance gene enables...causes the ribosome to translate Cas9-2A and the Puromycin resistance gene as separate peptides. PX459 This...predecessor, which had a point mutation in the puromycin resistance gene that made it less effective in...

  2. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    ...but was found to have a point mutation in the puromycin cassette that makes it less efficient in some ...with customizable sgRNA from the U6 promoter and puromycin resistance from the EF-1a promoter. It’s a 3rd...PX459) V2.0. It contains a point mutation in the puromycin cassette that makes it less efficient in some ...

  3. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...Nathans, Daniel. "Puromycin inhibition of protein synthesis: incorporation of puromycin into peptide chains...Yarmolinsky, Michael B., and L. Gabriel. "Inhibition by puromycin of amino acid incorporation into protein." Proceedings...

  4. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...stably integrate a reporter gene that contains a puromycin resistance gene followed by the target site (the...genome edit:  A donor plasmid that contains a puromycin resistance gene linked to an in-frame H2B-GFP....

  5. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...lentiviral supernatant and selected with puromycin. Puromycin-resistant cells were fixed and labeled with...or C-terminal HA tag. Selectable and stable: A puromycin resistance gene makes stable cell line creation...

  6. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...- and locus-specific gRNAs Step 4: Select for puromycin resistant cells Step 5: PCR amplify and sequence...cassette into the FBL locus. Upon sequencing of puromycin resistant clones, they found that 80% and 50% ...

  7. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...shared by bacteria and eukaryotes. For example, puromycin inhibits protein synthesis across kingdoms by ...targeting a shared aspect of ribosomal function. A puromycin resistance gene on your vector can protect both...

  8. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...marker such as puromycin, you can (also) select for the positive colonies by puromycin selection. You ...transfected cells (for example, plasmid with a puromycin resistance cassette, such as PX459). After you...

  9. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...kinase promoter drives expression of puromycin. Puro R Puromycin resistance gene for selection of pLKO...sulfate, and puromycin are located in the “Appendix”. F.2. Determining the Optimal Puromycin Concentration...differently to puromycin selection. Addgene strongly recommends that you determine the optimal puromycin concentration...to fresh puromycin-containing media every other day. f. The minimum concentration of puromycin that results...with finer increments of puromycin to determine a more precise optimal puromycin concentration.) F.3. Protocol...expression of the puromycin resistance gene. f. To select for infected cells, add puromycin to the media at...positive control for the puromycin selection. Days 4+: g. Change to fresh puromycin-containing media as needed...

  10. Lentiviral Prep Service

    Type
    Collection
    ... genes along with 1000 non-targeting controls Puromycin Doench , Root 73179 Human gRNA pooled library ... genes along with 1000 non-targeting controls Puromycin Doench , Root Human activation pooled libraries...56,762 unique sgRNAs targeting 18,885 human genes Puromycin Doench , Root Human inactivation pooled libraries...human genes along with 500 non-targeting controls Puromycin Doench , Root 92386 (Discontinued) Human CRISPR...human genes along with 500 non-targeting controls Puromycin Doench , Root Mouse knockout pooled libraries ... genes along with 1000 non-targeting controls Puromycin Doench , Root Cas9 Lentivirus Cas9 viruses can...

  11. Lentivirus Plasmids

    Type
    Collection
    ....1 puro 3rd U6-driven shRNA empty plasmid with puromycin selection. See plasmid 24150 for hygromycin selection...Tet-pLKO-puro 3rd Inducible expression of shRNA with puromycin selection. See plasmid 21916 for neomycin selection...19319 pLJM1-EGFP 3rd CMV-driven EGFP fusion with puromycin resistance. Can be used for cDNA expression. David...pLenti-puro 3rd CMV-driven expression of cDNA with puromycin selection. Ie-Ming Shih 25737 pSLIK-Hygro 3rd ...with neomycin selection. See plasmid 21915 for puromycin selection. Dmitri Wiederschain 36084 pLV-mCherry...Inducible lentiviral expression, TRE-gateway, with puromycin selection. See Root Lab pLEX and pLIX Gateway ...Encodes FUCCI reporters for cell cycle analysis. Puromycin selection. Kevin Brindle and Duncan Jodrell 17448...

  12. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...72255 Cre is coexpressed with tdTomato and Pac (puromycin N-acethyl-transferase) pCDH-CB-copGFP-T2A-iCre...pCDH-CB-FLPe-P2A-copGFP-T2A-Puro 72258 Expresses FLPe, copGFP and the puromycin resistance from the CB promoter pCDH-CB-FLPe-P2A-tdTomato...-copGFP-T2A-Puro 72263 Express copGFP and the puromycin resistance gene from the EF1 promoter pCDH-CMV-mCherry-T2A-Puro...pCDH-CMV-mCherry-T2A-Puro 72264 Express mCherry and puromycin resistance gene from the CMV promoter pCDH-CMV...

  14. Retrovirus Plasmids

    Type
    Collection
    ...creation of immortalized cells. See plasmid 1771 for puromycin selection, and plasmid 1774 for neomycin selection...expression with GFP expression. See plasmid 8452 for puromycin resistance. William Hahn 63704 pRetroX GFP T2A... For cloning and gene expression. Select with puromycin or screen for GFP. David Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE...

  17. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...80S; inhibits chain elongation. 50-500 ug/mL Puromycin pac HeLa, 293HEK, Jurkat T cells Inhibits protein...
Showing: 1 - 20 of 34 results